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Large-scale production and protein engineering of G protein-coupled receptors for structural studies.

Type of publication Peer-reviewed
Publikationsform Review article (peer-reviewed)
Publication date 2015
Author Milić Dalibor, Veprintsev Dmitry B,
Project NMR studies of GPCRs: Structure, dynamics and interactions with ligands and signaling proteins
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Review article (peer-reviewed)

Journal Frontiers in pharmacology
Volume (Issue) 6
Page(s) 66 - 66
Title of proceedings Frontiers in pharmacology
DOI 10.3389/fphar.2015.00066

Abstract

Structural studies of G protein-coupled receptors (GPCRs) gave insights into molecular mechanisms of their action and contributed significantly to molecular pharmacology. This is primarily due to technical advances in protein engineering, production and crystallization of these important receptor targets. On the other hand, NMR spectroscopy of GPCRs, which can provide information about their dynamics, still remains challenging due to difficulties in preparation of isotopically labeled receptors and their low long-term stabilities. In this review, we discuss methods used for expression and purification of GPCRs for crystallographic and NMR studies. We also summarize protein engineering methods that played a crucial role in obtaining GPCR crystal structures.
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