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NAB2 is a novel immune stimulator of MDA-5 that promotes a strong type I interferon response

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Oberson Anne, Spagnuolo Lorenzo, Puddinu Viola, Barchet Winfried, Rittner Karola, Bourquin Carole,
Project ProDoc Cell Migration Research Module 3: Soluble factors in Cell Migration
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Original article (peer-reviewed)

Journal Oncotarget
Volume (Issue) 9(5)
Page(s) 5641 - 5651
Title of proceedings Oncotarget
DOI 10.18632/oncotarget.v9i5

Open Access

URL https://archive-ouverte.unige.ch/unige:114099
Type of Open Access Repository (Green Open Access)

Abstract

Novel adjuvants are needed to increase the efficacy of vaccine formulations and immune therapies for cancer and chronic infections. In particular, adjuvants that promote a strong type I IFN response are required, since this cytokine is crucial for the development of efficient anti-tumoral and anti-viral immunity. Nucleic acid band 2 (NAB2) is a double-stranded RNA molecule isolated from yeast and identified as an agonist of the pattern-recognition receptors TLR3 and MDA-5. We compared the ability of NAB2 to activate innate immunity with that of poly(I:C), a well-characterized TLR3 and MDA-5 agonist known for the induction of type I IFN. NAB2 promoted stronger IFN-α production and induced a higher activation state of both murine and human innate immune cells compared to poly(I:C). This correlated with a stronger activation of the signalling pathway downstream of MDA-5, and IFN-α induction was dependent on MDA-5. Upon injection, NAB2 induced higher levels of serum IFN-α in mice than poly(I:C). These results suggest that NAB2 has the potential to become an efficient adjuvant for the induction of type-I IFN responses in therapeutic immunization against cancer or infections.
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