Data and Documentation
Open Data Policy
FAQ
EN
DE
FR
Suchbegriff
Advanced search
Publication
Back to overview
Trypanosoma brucei has a canonical mitochondrial processing peptidase
Type of publication
Peer-reviewed
Publikationsform
Original article (peer-reviewed)
Publication date
2012
Author
Desy S., Schneider A., Mani J.,
Project
Mitochondrial biogenesis in T. brucei - import of macromolecules
Show all
Original article (peer-reviewed)
Journal
Mol Biochem Parasitol
Volume (Issue)
185
Page(s)
161 - 4
Title of proceedings
Mol Biochem Parasitol
Abstract
Most mitochondrial matrix and inner membrane proteins have N-terminal presequences which serve as import signals. After import these presequences are cleaved by the heterodimeric mitochondrial processing peptidase. In the parasitic protozoa Trypanosoma brucei mitochondrial protein import relies on presequences that are much shorter than in other eukaryotes. How they are processed is unknown. The trypansomal genome encodes four open reading frames that are annotated as mitochondrial processing peptidase. Here we show that RNAi-mediated ablation of two of these proteins leads to a growth arrest and a concomitant accumulation of mitochondrial precursor proteins inside mitochondria. Import experiments using isolated mitochondria from RNAi cell lines reveals that both proteins are required for efficient import and processing of the tested precursor protein. Reciprocal immunoprecipitation demonstrates that the proteins interact with each other. In summary these results show that we have identified the two subunits of the trypanosomal mitochondrial processing peptidase.
-