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A plasma global metabolic profiling approach applied to an exercise study monitoring the effects of glucose, galactose and fructose drinks during post-exercise recovery

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Publication date 2010
Author Bruce Stephen J., Breton Isabelle, Décombaz Jacques E., Boesch Chris, Scheurer Eva, Montoliu Ivan, Rezzi Serge, Kochhar Sunil, Guy Philippe Alexandre,
Project Multi-nuclear magnetic resonance spectroscopy (MRS) and imaging (MRI) on a clinical whole-body MR-system: insulin resistance, ageing, and physical activity
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Original article (peer-reviewed)

Journal Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume (Issue) 878(29)
Page(s) 3015 - 3023
Title of proceedings Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
DOI 10.1016/j.jchromb.2010.09.004


A global metabolic profiling methodology based on gas chromatography coupled to time-of-flight mass spectrometry (GC-TOFMS) for human plasma was applied to a human exercise study focused on the effects of beverages containing glucose, galactose, or fructose taken after exercise and throughout a recovery period of 6. h and 45. min. One group of 10 well trained male cyclists performed 3 experimental sessions on separate days (randomized, single center). After performing a standardized depletion protocol on a bicycle, subjects consumed one of three different beverages: maltodextrin (MD). +. glucose (2:1 ratio), MD. +. galactose (2:1), and MD. +. fructose (2:1), consumed at an average of ~1.25. g of carbohydrate (CHO) ingested per minute. Blood was taken straight after exercise and every 45. min within the recovery phase. With the resulting blood plasma, insulin, free fatty acid (FFA) profile, glucose, and GC-TOFMS global metabolic profiling measurements were performed. The resulting profiling data was able to match the results obtained from the other clinical measurements with the addition of being able to follow many different metabolites throughout the recovery period. The data quality was assessed, with all the labelled internal standards yielding values of <15% CV for all samples (n= 335), apart from the labelled sucrose which gave a value of 15.19%. Differences between recovery treatments including the appearance of galactonic acid from the galactose based beverage were also highlighted. © 2010 Elsevier B.V.