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Rcount: simple and flexible RNA-Seq read counting.

Type of publication Peer-reviewed
Publikationsform Original article (peer-reviewed)
Author Schmid Marc W, Grossniklaus Ueli,
Project The Genetic and Molecular Basis of Gametogenesis and Maternal Effects in Arabidopsis
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Original article (peer-reviewed)

Journal Bioinformatics (Oxford, England)
Volume (Issue) 31(3)
Page(s) 436 - 7
Title of proceedings Bioinformatics (Oxford, England)
DOI 10.1093/bioinformatics/btu680

Open Access

URL http://bioinformatics.oxfordjournals.org/content/31/3/436.long
Type of Open Access Publisher (Gold Open Access)

Abstract

Analysis of differential gene expression by RNA sequencing (RNA-Seq) is frequently done using feature counts, i.e. the number of reads mapping to a gene. However, commonly used count algorithms (e.g. HTSeq) do not address the problem of reads aligning with multiple locations in the genome (multireads) or reads aligning with positions where two or more genes overlap (ambiguous reads). Rcount specifically addresses these issues. Furthermore, Rcount allows the user to assign priorities to certain feature types (e.g. higher priority for protein-coding genes compared to rRNA-coding genes) or to add flanking regions. Rcount provides a fast and easy-to-use graphical user interface requiring no command line or programming skills. It is implemented in C++ using the SeqAn (www.seqan.de) and the Qt libraries (qt-project.org). Source code and 64 bit binaries for (Ubuntu) Linux, Windows (7) and MacOSX are released under the GPLv3 license and are freely available on github.com/MWSchmid/Rcount. marcschmid@gmx.ch Test data, genome annotation files, useful Python and R scripts and a step-by-step user guide (including run-time and memory usage tests) are available on github.com/MWSchmid/Rcount.
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