Project

Back to overview

IMAGO : Imaging and Modeling Growth and plasticity in plant Ovules

English title IMAGO : Imaging and Modeling Growth and plasticity in plant Ovules
Applicant Baroux Célia
Number 170167
Funding scheme Project funding
Research institution Institut für Pflanzen- und Mikrobiologie Universität Zürich
Institution of higher education University of Zurich - ZH
Main discipline Embryology, Developmental Biology
Start/End 01.11.2016 - 31.10.2019
Approved amount 292'420.00
Show all

All Disciplines (5)

Discipline
Embryology, Developmental Biology
Cellular Biology, Cytology
Mathematics
Molecular Biology
Genetics

Keywords (7)

germ cell fate; growth model; apomixis; confocal imaging; lightsheet imaging; morphogenesis; plant ovule

Lay Summary (French)

Lead
La reproduction clonale par l’intermédiaire de graines, ou apomixie, est un phénomène que l’on rencontre chez certaines plantes supérieures. L’apomixie représente un potentiel énorme pour la sécurité alimentaire et l’agriculture durable. Développer des variétés de plantes d'intérêt agronomique se reproduisant par apomixie révolutionnerait les pratiques de sélection variétale et production de graines. L’apomixie permettrait la fixation aisée de génotypes et traits hybrides, et notamment la vigueur hybride. Cette forme de reproduction existe naturellement dans de nombreuses familles de plantes, mais pas chez les espèces cultivées. De ce fait, un large effort anime une communauté internationale de laboratoires académiques et privés pour comprendre les mécanismes qui sous-tendent ce phénomène.
Lay summary

Le premier évènement différenciant la reproduction sexuée de l’apomixie a lieu au sein de l’organe reproducteur femelle, l’ovule, où une cellule unique transite d’une identité cellulaire somatique vers une identité germinale. Lorsque la proliferation et la croissance des cellules de l’ovule sont perturbées, s’en suit un développement ressemblant à celui d’ovules apomictiques suggérant une relation fonctionnelle entre la morphogenèse précoce de l’ovule et la transition vers l’identité germinale . Mais ce lien reste largement inexplorée, au même titre que la plasticité existant entre développement reproducteur sexué et apomictique. L’émergence de techniques d’imagerie non invasive, a haute résolution et en trois dimensions, d’outils de modélisation, en combinaison à des approches fonctionnelles génétiques et pharmacologiques, permettent collectivement aujourd’hui d’aborder ces questions. 

Le but du projet IMAGO est de construire un cadre de référence visant a élucider les règles de développement de la croissance de l’ovule, et déterminer de quelle façon cette géométrie contribue a l’établissement du devenir germinal dans l’ovule. L’ovule est un objet biologique relativement simple, particulièrement adapté à la modélisation, permettant de mathématiser différentes hypothèses de croissance, en 2D ou 3D et au cours du temps. Ces hypothèses seront ensuite testées fonctionnellement grâce aux outils génétiques disponibles chez l’espèce modèle étudiée Arabidopsis. La généricité des résultats obtenus sera testée chez une céréale cultivée sexuée, le maïs; et un modèle de graminée apomictique, Paspalum. Le projet IMAGO, réunissant biologistes de la reproduction et modélisateurs, vise a produire des connaissances et outils pluridisciplinaires pour comprendre la plasticité du développement apomictique et transférer cette capacité aux espèces cultivées.

Direct link to Lay Summary Last update: 26.10.2016

Responsible applicant and co-applicants

Employees

Publications

Publication
Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium
Hernandez-Lagana Elvira, Mosca Gabriella, Mendocilla-Sato Ethel, Pires Nuno, Frey Anja, Giraldo-Fonseca Alejandro, Michaud Caroline, Grossniklaus Ueli, Hamant Olivier, Godin Christophe, Boudaoud Arezki, Grimanelli Daniel, Autran Daphné, Baroux Célia (2021), Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium, in eLife, 10, 1.
H3.1 Eviction Marks Female Germline Precursors in Arabidopsis
Hernandez-Lagana Elvira, Autran Daphné (2020), H3.1 Eviction Marks Female Germline Precursors in Arabidopsis, in Plants, 9(10), 1322-1322.
Plant Germline Development
Mendocilla-Sato Ethel, She Wenjing, Baroux Célia (2017), Plant Germline Development, Springer New York, New York, NY.
Analysis of 3D Cellular Organization of Fixed Plant Tissues Using a User-guided Platform for Image Segmentation
Mendocilla Sato Ethel, Baroux Célia (2017), Analysis of 3D Cellular Organization of Fixed Plant Tissues Using a User-guided Platform for Image Segmentation, in BIO-PROTOCOL, 7(12), 1-n.

Datasets

Arabidopsis ovule primordium stages 0-I to 2-II, wild-type Col_0, Ws-4 and botero (bot1-7) mutant

Author Baroux, Celia
Publication date 11.01.2021
Persistent Identifier (PID) https://doi.org/10.5061/dryad.02v6wwq2c
Repository Dryad
Abstract
This image resource describes the development of ovule primordia from stages 0-I to 2-I in 3D and at cellular resolution. The images were segmented using ImarisCell (BitplaneAG) and annotated as described in the associated manuscript Hernandez-Lagana et al. eLife 2021 DOI: 10.7554/eLife.66031

Collaboration

Group / person Country
Types of collaboration
ENS Lyon France (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Exchange of personnel
INRIA France (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Institut de Recherche et Développement (IRD) France (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel
Bitplane R&D Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Industry/business/other use-inspired collaboration

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
FOR2581 Plant Growth and Form. Symposium on Quantitative Plant Morphodynamics Poster Imaging in 4D ovule primordium development in Arabidopsis 09.09.2019 Heidelberg, Germany Autran Daphné;
International Symposium On Plant Growth And Form. Talk given at a conference Role of ovule growth dynamics on germline fate establishment in Arabidopsis 09.09.2019 Heidelberg, Germany Baroux Célia;
International Symposium On Plant Growth And Form Poster MorphomechanX, a novel tool to model plant morphogenesis and its application on A. thaliana ovule development 09.09.2019 Heidelberg, Germany Mosca Gabriella;
Workshop on Molecular Mechanisms Controlling Flower Development Talk given at a conference Morphological principles modulating reproductive fate and plasticity within the Arabidopsis ovule 18.06.2019 Presqu’île de Giens, France Autran Daphné;
Annual Meeting of the Plant Morphodynamics DFG‐ funded Consortium FOR2581. Talk given at a conference Role of ovule architecture on SMC fate establishment. 26.03.2019 Freysing, Germany Baroux Célia;
Seminar Series, ENS Lyon. Individual talk Modeling plant morphogenesis (and bio-­‐mechanics) with MorphoMechanX: general introduction and work in progress on A. thaliana ovule development 07.03.2019 Lyon, France Mosca Gabriella;
LANGEBIO CINVESTAV seminars series Individual talk Role of early ovule architecture in female germ cell fate in Arabidopsis 14.01.2019 Irapuato, Guanajuato, Mexico Autran Daphné;
Taller de Biología Molecular de Plantas IICAR-IBR-CEFOBI Talk given at a conference Rol de la arquitectura temprana del ovulo en la determinacion del destino de la linea germinal femenina en Arabidopsis 06.12.2018 Rosario, Argentina Autran Daphné;
Ciclo de seminarios sobre avances en la caracterización genética y molecular de la apomixis, IICAR-CONICET Talk given at a conference Role of early ovule architecture in female germ cell fate in Arabidopsis 26.11.2018 Rosario, Argentina Autran Daphné;
IPMB International Plant Molecular Biology meeting Poster Dynamics of cell division patterns and their role on MMC fate acquisition during ovule primordium development in Arabidopsis 05.08.2018 Montpellier, France Autran Daphné;
Plant Biomechanics Conference Poster MorphoDynamX, a novel, user-­‐friendly tool for plant morphogenesis and a test case on A. thaliana ovule development 04.08.2018 Montreal, Canada Mosca Gabriella;
SEB satellite meeting “Advances in plant reproduction: from gametes to seeds.” Talk given at a conference Early ovule architecture determines female germ cell fate and unicity in Arabidopsis 30.06.2018 Firenze, Italy Autran Daphné;
EMBO practical course - 3D developmental imaging Talk given at a conference Dynamics of cell division patterns and their role on female Spore Mother Cell fate acquisition during ovule primordium development in Arabidopsis 30.06.2018 Lisboa, Portugal Autran Daphné;
ICAR International Conference on Arabidopsis Research Individual talk Early ovule architecture determines female germ cell fate and unicity in Arabidopsis 22.06.2018 Turku, Finland Autran Daphné;
IPMB Institute Seminar Individual talk Cellular dynamics during female germline development in Arabidopsis thaliana 28.03.2018 Zürich, Switzerland Mendocilla-Sato Ethel;
Swiss Plant Science Web Meeting Individual talk How shape constrains cell fate: a 3D look at ovule growth in Arabidopsis 26.01.2017 Leukerbad, Switzerland Baroux Célia;


Self-organised

Title Date Place
IMAGO Final Meeting 26.03.2019 Lyon, France
IMAGO Mid-Term Meeting 04.06.2018 Lyon, France
IMAGO Kick-Off Meeting 11.06.2017 Lyon, France

Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Fascination of Plant Days Performances, exhibitions (e.g. for education institutions) 18.05.2019 Zürich, Switzerland Schubert Jasmin; Baroux Célia;
100 ways of thinking Performances, exhibitions (e.g. for education institutions) 23.11.2018 Zürich, Switzerland Baroux Célia;
PSC PhD course "Advanced microscopy imaging of plant tissues and image processing" Performances, exhibitions (e.g. for education institutions) 13.04.2018 Zürich, Switzerland Baroux Célia;


Communication with the public

Communication Title Media Place Year
Video/Film IMAGO International 2021
Talks/events/exhibitions FascinationOfPlantDays German-speaking Switzerland 2019
Talks/events/exhibitions 100 Ways Of Thinking German-speaking Switzerland 2018

Awards

Title Year
PostDoc Fellowship, Forschungskredit University of Zürich 2019

Associated projects

Number Title Start Funding scheme
149974 Mechanisms and role of linker histones dynamics in plant reproduction 01.01.2014 Project funding

Abstract

Abstract. Apomixis is a clonal mode of seed formation occurring naturally in many plant species but not in major crop plants. Revolutionizing agriculture practices via engineered apomixis bear great promises yet requires a deep understanding of its mechanisms. One goal, notably, is to elucidate the processes leading to ectopic germ cell fate in the ovule of so-called aposporous plant species. Despite considerable progress in deciphering genetic and epigenetic contributions we are critically lacking a developmental, morphogenetic level. Yet, recent observations from the partners’ groups indeed suggest a link between tissue patterning events in the early ovule and ectopic germ cells. By exploiting the advent of high-resolution 3D imaging, image processing, and tissue growth modeling approaches, together with functional genetics, IMAGO proposes to elucidate patterning rules governing ovule growth, and their contribution to female germ cell fate acquisition. Ultimately, IMAGO provides novel tools and knowledge contributing innovation towards engineering apomixis.Objectives. IMAGO combines high-resolution static and real-time 3D imaging, quantitative image processing, cell-based growth models and functional approaches to (1) define cellular growth patterns in the ovule primordium using quantitative imaging (2) test patterning rules in silico by cell-based growth models (3) validate patterning rules in vivo using genetic and pharmacological perturbations; and analyzing sexual and apomictic grasses (Maize and Paspalum).Partners and Synergy. IMAGO is an interdisciplinary project that requires the synergistic contributions of Partner 1: Daphné Autran (French coordinator), Daniel Grimanelli (participating scientist), Institut de Recherche pour le Développement (IRD Montpellier, France); Partner 2: Célia Baroux (Swiss coordinator), Department of Plant and Microbial Biology (University of Zürich, Switzerland); Partner 3: Christophe Godin, INRIA, France; Partner 4 (external advisors): Olivier Hamant and Arezki Boudaoud (ENS Lyon, France). Partner 1 and 2 are developmental biologists sharing expertise in genetic, molecular and cytological approaches to analyze plant reproduction. Specific and complementary expertise are brought by Partner 1 on maize and apomictic reproduction, by Partner 2 on high-resolution imaging and quantitative image processing, by Partner 3 on 4D image processing and 3D topological modeling, and by Partners 4 on 2D modeling of plant growth patterns. Impact. (i) high-training benefits for the recruited scientists: interdisciplinary research combining cell biology, high-resolution imaging and mathematical modeling in top-excellence institutes, (ii) novel tools and methodologies to study ovule development through imaging and modeling at the cellular and tissue level, in 4D, (iii) a novel conceptual framework integrating growth dynamics and germ cell fate acquisition in the ovule, linking therein two traditionally disconnected research fields, (iv) novel insights on the developmental plasticity of plant ovule contributing the long-term goal of engineering apomixis in crop plants, (v) dissemination: IMAGO ambitions several peer-reviewed scientific articles and produce attractive image material suitable for public outreach. In addition, dedicated efforts to disseminate the knowledge developed by IMAGO to the European plant breeding community, in both academy and industry will be undertaken, to raise their awareness on progress toward the development of apomictic crops.
-