Antimicrobial resistance ecology; Horizontal gene transfer; Mobile genetic elements; Resistome; Metaviromics; Metagenomics; Plasmidome
Gómez SanzElena, Haro-MorenoJose Manuel, JensenSlade O., Roda-GarciaJuan J., Lopez-PerezMario (2021), The Resistome and Mobilome of Multidrug-Resistant Staphylococcus sciuri C2865 Unveil a Transferable Trimethoprim Resistance Gene, Designated dfrE, Spread Unnoticed, in mSystems
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Göller1 Pauline C, Elsener Tabea, Lorgé Dominic, Radulovic Natasa, Bernardi Viona, Naumann Annika, Amri Nesrine, Khatchatourova Ekaterina, Hernandes Coutinho Felipe, Loessner Martin J, Gómez Sanz Elena (2021), Natural phage communities crosslink different species within the genus Staphylococcus, 1.
Göller Pauline C., Haro-Moreno Jose M., Rodriguez-Valera Francisco, Loessner Martin J., Gómez-Sanz Elena (2020), Uncovering a hidden diversity: optimized protocols for the extraction of dsDNA bacteriophages from soil, in Microbiome
, 8(1), 17-17.
Gómez-Sanz Elena, Haro-Moreno Jose Manuel, Jensen Slade O, Roda-Garcia Juan Jose, Lopez-Perez Mario (2020), Staphylococcus sciuri C2865 from a distinct subspecies cluster as reservoir of the novel transferable trimethoprim resistance gene, dfrE, and adaptation driving mobile elements, 1.
Göller Pauline C, Haro-Moreno Jose M, Rodriguez-Valera Francisco, Loessner Martin J, Gómez-Sanz Elena (2019), Uncovering a hidden diversity: optimized protocols for the extraction of dsDNA bacteriophages from soil, in BioRxiv
GöllerPauline C, ElsenerTabea, LorgéDominic, RadulovicNatasa, BernardiViona, NaumannAnnika, AmriNesrine, KhatchatourovaEkaterina, Hernandes CoutinhoFelipe, LoessnerMartin J, Gómez SanzElena, Multi-species host range of staphylococcal phages isolated from wastewater, in Nature Communications
There is an imperative need to gain knowledge on the spread and evolution of antimicrobial resistance (AMR). Recent evidence from environmental, animal and human microbial studies shows that AMR is ancient and envi-ronments without contact with anthropogenic antibiotics possess abundant AMR genes. Bacteriophages are the most abundant entities in Earth and recent studies provide indirect evidence for the major role of transduction in the dissemination of AMR genes. Hence, phages might be of critical importance for evading one of the bottle-necks (ecological connectivity) that modulates the transmission of AMR genes from natural environments to animal and human biomes. In this proposal, a holistic and multidisciplinary approach that combines molecular genetics, genomics, metagenomics (and other -omics approaches), in-vitro experimental analysis, computational biology, bioinformatics analysis, and statistics will be conducted to measure the potential of phages as vehicles for antimicrobial resistance (AMR) transmission and spread in environmental systems with different antibiotic selective pressure. This comprehensive approach will allow us to better understand the phage-bacteria interac-tions that may drive AMR spread in different ecosystems. From this understanding we expect to be able to design and develop new tools and contingency actions for the control of AMR in clinical and veterinary practice as well as in agriculture.