Epigenetics; Chromatin; Linker Histones; plant reproduction; Arabidopsis; developmental transitions
Rutowicz Kinga, Lirski Maciej, Mermaz Benoît, Teano Gianluca, Schubert Jasmin, Mestiri Imen, Kroteń Magdalena A., Fabrice Tohnyui Ndinyanka, Fritz Simon, Grob Stefan, Ringli Christoph, Cherkezyan Lusik, Barneche Fredy, Jerzmanowski Andrzej, Baroux Célia (2019), Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis, in Genome Biology
, 20(1), 157-157.
She Wenjing, Baroux Célia, Grossniklaus Ueli (2018), Cell-Type Specific Chromatin Analysis in Whole-Mount Plant Tissues by Immunostaining., in Baroux Célia, Bemer Marian (ed.), Springer, NY, 1675, 443-454.
Baroux Célia, Schubert Veit (2018), Technical Review: Microscopy and Image Processing Tools to Analyze Plant Chromatin: Practical Considerations., in Bemer Marian, Baroux Célia (ed.), Springer, NY, 1675, 537-589.
Ashenafi Mariam, Baroux Célia (2018), Automated 3D Gene Position Analysis Using a Customized Imaris Plugin: XTFISHInsideNucleus, in Bemer Marian, Baroux Célia (ed.), Springer, New York, 1.
Fabrice T.N, Cherkezeyan L., Ringli C., Baroux C. (2018), Transmission Electron Microscopy Imaging to analyse chromatin density distribution at the nanoscale level, in Bemer Marian, Baroux Célia (ed.), Springer, New York, 1.
Vogiatzaki Evangelia, Baroux Célia, Jung Ji-Yul, Poirier Yves (2017), PHO1 Exports Phosphate from the Chalazal Seed Coat to the Embryo in Developing Arabidopsis Seeds., in Current biology : CB
, 27(19), 2893.
Kotlinski M., Knizewski L., Muszewska A., Rutowicz K., Lirski M., Schmidt A., Baroux C., Ginalski K., Jerzmanowski A. (2017), Phylogeny-based systematization of arabidopsis proteins with histone H1 globular domain, in Plant Physiology
, 174(1), 27-34.
Barneche F., Baroux C. (2017), Unreeling the chromatin thread: A genomic perspective on organization around the periphery of the Arabidopsis nucleus, in Genome Biology
, 18(1), 1-3.
Baroux Célia, Pecinka Ales, Fuchs Jörg, Kreth Gregor, Schubert Ingo, Grossniklaus Ueli (2016), Non-random chromosome arrangement in triploid endosperm nuclei., in Chromosoma
Pires Nuno D, Bemer Marian, Müller Lena M, Baroux Célia, Spillane Charles, Grossniklaus Ueli (2016), Quantitative Genetics Identifies Cryptic Genetic Variation Involved in the Paternal Regulation of Seed Development., in PLoS genetics
, 12(1), 1005806-1005806.
Baroux Celia, Autran Daphne (2015), Chromatin dynamics during cellular differentiation in the female reproductive lineage of flowering plants, in Plant Journal
, 83(1), 160-176.
She Wenjing, Baroux Celia (2015), Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis, in Frontiers in Plant Science
, 6, 1/294-7.
Baroux Celia, Grossniklaus Ueli (2015), The Maternal-to-Zygotic Transition in Flowering Plants: Evidence, Mechanisms, and Plasticity., in Current topics in developmental biology
, 113, 351-71.
She Wenjing, Grimanelli Daniel, Baroux Celia (2014), An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis, in Jove-Journal of Visualized Experiments
, (88), 51530.
She Wenjing, Baroux Celia (2014), Chromatin dynamics during plant sexual reproduction, in Frontiers in Plant Science
, 5, 1/534.
Varas Javier, Baroux Celia (2014), Meiotic chromosome movements in plants, a puppet show?, in Frontiers in Plant Science
, 5, 1/502.
Unlike most animals, plants retain a remarkable cellular plasticity post-embryogenesis. Throughout their life cycle, plants undergo several developmental phase transitions producing new physiological states (e.g. dormancy), organs (e.g. flowers) or specialized cells (e.g. gametes) that were not established at embryogenesis. Critically, these transitions are associated with large-scale and dynamic event of chromatin reorganization. Current efforts are aiming at establishing the causal link between these events and cell fate acquisition. During our former SNF-funded project, we have shown that the somatic-to-reproductive fate transition in Arabidopsis is associated with chromatin reprogramming which partly establishes the post-meiotic, gametophytic fate. H1 depletion from the chromatin of spore mother cells is the earliest event of chromatin reprogramming. No canonical H1 variants is detectable throughout the female gametophyte and embryogenesis, raising the question of the function of this particular chromatin state, particularly with regard to epigenetic reprogramming. We now aim at elucidating (i) the mechanisms and role of H1 dynamics in plant reproduction and (ii) the role of putative germline specific H1-related variants. We will use molecular genetics approaches, notably to misregulate H1 variants in a conditional and cell-specific manner, together with standard techniques in developmental biology to analyse subtle developmental and cell fate alterations in the mutants, but also high-resolution imaging of fluorescent reporters and chromatin markers, as well as transcriptome studies and FRAP analyses of the H1 variants to assess their chromatin binding properties. Collectively, this project will provide the first insight on the developmental role of H1 dynamics during cellular reprogramming in plants.