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Molecular interactions of wheat and fungal pathogens: Lr34-based, durable resistance and the wheat-powdery mildew pathosystem

Applicant Keller Beat
Number 144081
Funding scheme Project funding
Research institution Institut für Pflanzen- und Mikrobiologie Universität Zürich
Institution of higher education University of Zurich - ZH
Main discipline Botany
Start/End 01.01.2013 - 31.12.2015
Approved amount 888'312.00
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Keywords (8)

Disease resistance; Wheat; Cereals; Fungal pathogens; Rust; Powdery mildew; Durable; Genomics

Lay Summary (German)

Lead
Pilzliche Krankheitserreger verursachen weltweit grosse Verluste in Qualität und Ertrag von landwirtschaftlichen Nutzpflanzen. Zu den wichtigsten Pathogenen in Weizen gehören Rostpilze und Mehltau. Es gibt in Weizen genetische Ressourcen, die natürlicherweise eine gewisse Krankheitsresistenz haben. Über die molekularen Wirkungsmechanismen dieser Resistenz ist wenig bekannt. Insbesondere ist nicht klar, ob sie in andere Kulturpflanzen übertragen werden und genetisch noch verbessert werden könnte.
Lay summary

Molecular interactions of wheat and fungal pathogens: Lr34-based, durable resistance and the wheat-powdery mildew pathosystem

Molekulare Interaktionen von Weizen und pilzlichen Krankheitserregern: Lr34-basierte, dauerhafte Resistenz und das Weizen-Mehltau Pathosystem

 

Inhalt und Ziel des Forschungsprojekts

Wir verfolgen mit diesem Projekt zwei Ziele: einerseits soll der molekulare Mechanismus der dauerhaften, partiell wirksamen Rostresistenz basierend auf dem natürlicherweise vorkommenden Resistenzgen Lr34 verstanden werden. Andererseits wollen wir erforschen, welche molekularen Komponenten von Weizen und Mehltau miteinander interagieren. Dabei sollen Hypothesen entwickelt und später getestet werden, wie diese Resistenz verbessert werden könnte, damit sich der Krankheitserreger nicht an Resistenzgene anpassen kann.

 

Wissenschaftlicher und gesellschaftlicher Kontext des Forschungsprojekts

Die Analyse der molekularen Grundlagen der pflanzlichen Krankheitsresistenz ist weltweit ein sehr aktives Forschungsgebiet. Dabei besteht ein grosser Nachholbedarf bei der Arbeit mit Kulturpflanzen, da bis heute sehr stark mit Modellpflanzen gearbeitet wurde. Das Verständnis von natürlich vorkommender Krankheitsresistenz ist wichtig für die zukünftige verbesserte Nutzung von solchen genetischen Möglichkeiten zur Krankheitsbekämpfung, besonders im Hinblick auf Ertragssicherheit und die Reduktion des Einsatzes von Fungiziden in der Landwirtschaft.

Direct link to Lay Summary Last update: 27.12.2012

Responsible applicant and co-applicants

Employees

Publications

Publication
Genetic and molecular characterization of a locus involved in avirulence of Blumeria graminis f. sp. tritici on wheat Pm3 resistance alleles
Parlange F., Roffler S., Menardo F., Ben-David R., Bourras S., McNally K. E., Oberhaensli S., Stirnweis D., Buchmann G., Wicker T., Keller B. (2015), Genetic and molecular characterization of a locus involved in avirulence of Blumeria graminis f. sp. tritici on wheat Pm3 resistance alleles, in Fungal Genet Biol, 82, 181-92.
Multiple Avirulence Loci and Allele-Specific Effector Recognition Control the Pm3 Race-Specific Resistance of Wheat to Powdery Mildew
Bourras S., McNally K. E., Ben-David R., Parlange F., Roffler S., Praz C. R., Oberhaensli S., Menardo F., Stirnweis D., Frenkel Z., Schaefer L. K., Fluckiger S., Treier G., Herren G., Korol A. B., Wicker T., Keller B. (2015), Multiple Avirulence Loci and Allele-Specific Effector Recognition Control the Pm3 Race-Specific Resistance of Wheat to Powdery Mildew, in Plant Cell, 27, 2991-3012.
The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase
Hurni S., Scheuermann D., Krattinger S. G., Kessel B., Wicker T., Herren G., Fitze M. N., Breen J., Presterl T., Ouzunova M., Keller B. (2015), The maize disease resistance gene Htn1 against northern corn leaf blight encodes a wall-associated receptor-like kinase, in Proc Natl Acad Sci U S A, 112, 8780-5.
The wheat durable, multipathogen resistance gene Lr34 confers partial blast resistance in rice
Krattinger S. G., Sucher J., Selter L. L., Chauhan H., Zhou B., Tang M., Upadhyaya N. M., Mieulet D., Guiderdoni E., Weidenbach D., Schaffrath U., Lagudah E. S., Keller B. (2015), The wheat durable, multipathogen resistance gene Lr34 confers partial blast resistance in rice, in Plant Biotechnol J, 1467.
The wheat resistance gene Lr34 results in the constitutive induction of multiple defense pathways in transgenic barley
Chauhan H., Boni R., Bucher R., Kuhn B., Buchmann G., Sucher J., Selter L. L., Hensel G., Kumlehn J., Bigler L., Glauser G., Wicker T., Krattinger S. G., Keller B. (2015), The wheat resistance gene Lr34 results in the constitutive induction of multiple defense pathways in transgenic barley, in Plant J, 84, 202-15.
High-resolution analysis of a QTL for resistance to Stagonospora nodorum glume blotch in wheat reveals presence of two distinct resistance loci in the target interval
Shatalina M., Messmer M., Feuillet C., Mascher F., Paux E., Choulet F., Wicker T., Keller B. (2014), High-resolution analysis of a QTL for resistance to Stagonospora nodorum glume blotch in wheat reveals presence of two distinct resistance loci in the target interval, in Theor Appl Genet, 127, 573-86.
Suppression among alleles encoding nucleotide-binding-leucine-rich repeat resistance proteins interferes with resistance in F1 hybrid and allele-pyramided wheat plants
Stirnweis D., Milani S. D., Brunner S., Herren G., Buchmann G., Peditto D., Jordan T., Keller B. (2014), Suppression among alleles encoding nucleotide-binding-leucine-rich repeat resistance proteins interferes with resistance in F1 hybrid and allele-pyramided wheat plants, in Plant J, 79, 893-903.
The powdery mildew resistance gene Pm8 derived from rye is suppressed by its wheat ortholog Pm3
Hurni S., Brunner S., Stirnweis D., Herren G., Peditto D., McIntosh R. A., Keller B. (2014), The powdery mildew resistance gene Pm8 derived from rye is suppressed by its wheat ortholog Pm3, in Plant J, 79, 904-13.
Genotype-specific SNP map based on whole chromosome 3B sequence information from wheat cultivars Arina and Forno
Shatalina M., Wicker T., Buchmann J. P., Oberhaensli S., Simkova H., Dolezel J., Keller B. (2013), Genotype-specific SNP map based on whole chromosome 3B sequence information from wheat cultivars Arina and Forno, in Plant Biotechnol J, 11, 23-32.
Recent emergence of the wheat Lr34 multi-pathogen resistance: insights from haplotype analysis in wheat, rice, sorghum and Aegilops tauschii
Krattinger S. G., Jordan D. R., Mace E. S., Raghavan C., Luo M. C., Keller B., Lagudah E. S. (2013), Recent emergence of the wheat Lr34 multi-pathogen resistance: insights from haplotype analysis in wheat, rice, sorghum and Aegilops tauschii, in Theor Appl Genet, 126, 663-72.
Rye Pm8 and wheat Pm3 are orthologous genes and show evolutionary conservation of resistance function against powdery mildew
Hurni S., Brunner S., Buchmann G., Herren G., Jordan T., Krukowski P., Wicker T., Yahiaoui N., Mago R., Keller B. (2013), Rye Pm8 and wheat Pm3 are orthologous genes and show evolutionary conservation of resistance function against powdery mildew, in Plant J, 76, 957-69.
The wheat Lr34 gene provides resistance against multiple fungal pathogens in barley
Risk J. M., Selter L. L., Chauhan H., Krattinger S. G., Kumlehn J., Hensel G., Viccars L. A., Richardson T. M., Buesing G., Troller A., Lagudah E. S., Keller B. (2013), The wheat Lr34 gene provides resistance against multiple fungal pathogens in barley, in Plant Biotechnol J, 11, 847-54.
The wheat powdery mildew genome shows the unique evolution of an obligate biotroph
Wicker T., Oberhaensli S., Parlange F., Buchmann J. P., Shatalina M., Roffler S., Ben-David R., Dolezel J., Simkova H., Schulze-Lefert P., Spanu P. D., Bruggmann R., Amselem J., Quesneville H., Ver Loren van Themaat E., Paape T., Shimizu K. K., Keller B. (2013), The wheat powdery mildew genome shows the unique evolution of an obligate biotroph, in Nat Genet, 45, 1092-6.

Collaboration

Group / person Country
Types of collaboration
Dr. Pietro Spanu Great Britain and Northern Ireland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Dr. Patrick Schweizer, IPK Gatersleben Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Dr. Evans Lagudah, CSIRO, Canberra Australia (Oceania)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Dr. Andreas Graner/Dr. Nils Stein Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Prof. Schulze-Lefert, MPI, Köln Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Prof. Enrico Martinoia Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel
Milena Ouzonova, KWS Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel
- Industry/business/other use-inspired collaboration
Dr. Ravi Singh Mexico (North America)
- in-depth/constructive exchanges on approaches, methods or results

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Zurich host-microbes genomics workshop Talk given at a conference Small RNA sequencing analysis of the wheat powdery mildew interaction. 17.12.2015 zurich, Switzerland Bourras Salim;
Cold Spring Harbor Laboratory Conference: Plant Genomes & Biotechnology: From Genes to Networks Talk given at a conference Genomic approaches to improve durable resistance in cereals 05.12.2015 Cold Spring Harbor, United States of America Keller Beat;
PSC Symposium on Unlocking the potential of diversity Poster Evidence in powdery mildew of effector diversity as a means to evade resistance responses in wheat 03.12.2015 Zurich, Switzerland Mc Nally Kaitlin;
PSC Symposium on Unlocking the potential of diversity Poster The functional transfer of the wheat gene Lr34 into rice and its resistance against rice blast 03.12.2015 Zurich, Switzerland Sucher Justine;
Agricultural Genomics 2015 - from variation to improved production. Nature Genetics Conference Talk given at a conference Molecular diversity of fungal disease resistance genes in cereals and their applications in breeding 17.11.2015 Dapeng District, Shenzhen, China Keller Beat;
Hubei Academy of Agriculture Individual talk Molecular analysis of cereal crop interactions with fungal pathogens 13.11.2015 Wuhan, China Keller Beat;
The Sainsbury Laboratory Individual talk Molecular analysis of cereal crop interactions with fungal pathogens: From the field to the lab (and back…) 29.09.2015 Norwich, Great Britain and Northern Ireland Keller Beat;
18th Genome Research GPZ-conference: New Targets for Crops of the Future Talk given at a conference Genetic diversity in wheat: genomic approaches for its characterization and use in breeding 23.09.2015 Düsseldorf, Germany Keller Beat;
110th Congresso della Societa Botanica Italiana Talk given at a conference From the lab to the field: how to improve disease resistance in a changing environment 16.09.2015 Pavia, Italy Keller Beat;
Barley PhD Summer School "Barlomics": IPK Gatersleben Talk given at a conference Molecular breeding approaches to improve fungal disease resistance in wheat 14.09.2015 Gatersleben, Germany Keller Beat;
Conference: Novel approaches to achieve durable disease resistance, Wageningen University Talk given at a conference Transgenic and genomics-oriented breeding approaches to improve durable fungal disease resistance in wheat 03.09.2015 Wageningen , Netherlands Keller Beat;
NLR Biology in Plants and Animals, Workshop organized by the Max-Planck-Institute Cologne Talk given at a conference Allelic interactions of the wheat Pm3 multi-allelic series of powdery mildew resistance genes 05.05.2015 Schloss Ringberg, Germany Keller Beat;
Seminar, Technical University München, Individual talk Molecular approaches towards durable fungal disease resistance in wheat 16.04.2015 Munich, Germany Keller Beat;
23. Jahrestagung der Schweiz. Gesellschaft für Pflanzenbauwissenschaften (SGPW) Talk given at a conference Potential gentechnischer Methoden für die Resistenzzüchtung von Weizen 20.03.2015 Zollikofen, Switzerland Keller Beat;
Zurich mycology symposium 2015 Talk given at a conference Unravelling the genetics and molecular biology of avirulence in powdery mildew against the Pm3 multiallelic, race-specific resistance of wheat. 16.01.2015 zurich, Switzerland Bourras Salim;
BIO 752 Guest Seminars HS 2014, Institute of Systematic Botany, University of Zurich Individual talk Domestication and agriculture shape the co-evolution of wheat and its fungal pathogens 10.11.2014 Zurich, Switzerland Keller Beat;
PLANT FELLOWS Annual Meeting Talk given at a conference Molecular analysis of wheat disease resistance against powdery mildew 29.09.2014 Rigi Kulm, Switzerland Keller Beat; Sanchez Martin Javier;
GPZ Congress 2014, Gesellschaft für Pflanzenzüchtung Talk given at a conference Lessons learnt from transgenic approaches for classical resistance breeding in wheat 24.09.2014 Kiel, Germany Keller Beat;
18th Evolutionary Biology Meeting Talk given at a conference Evolutionary genomics of the powdery mildew pathogen and its adaptation to different cereal host species 17.09.2014 Marseilles, France Keller Beat;
4th international powdery mildew workshop. Talk given at a conference Unravelling the genetics and molecular biology of avirulence in powdery mildew against the Pm3 multiallelic, race-specific resistance of wheat. 14.09.2014 London, Great Britain and Northern Ireland Bourras Salim;
SUSTAIN Action workshop on Pathogen-informed strategies for sustainable broad-spectrum crop resistance Poster Map based cloning of a candidate avirulence partner of the wheat powdery mildew resistance gene Pm3 10.09.2014 Bucharest, Romania Mc Nally Kaitlin;
University of Adelaide, Australian Plant Funcitional Genomics Centre Individual talk Genomics-assisted breeding approaches to improve durable fungal disease resistance in wheat 18.08.2014 Adelaide, Australia Keller Beat;
International Association for Plant Biotechnology, Congress 2014 Talk given at a conference Transgenic and genomics-assisted breeding approaches to improve durable fungal disease resistance in wheat 11.08.2014 Melbourne, Australia Keller Beat;
XVI International Congress on Molecular Plant-Microbe Interactions Talk given at a conference The wheat broad spectrum disease resistance gene Lr34 is functionally transferable to other cereals 10.07.2014 Rhodos, Greece Sucher Justine;
International Triticeae Mapping Initiative (ITMI)-Eucarpia Conference Talk given at a conference Suppression among alleles of an NB-LRR resistance gene interferes with resistance in F1 hybrids and allele-pyramided wheat plants 01.07.2014 Wernigerode, Germany Keller Beat;
EUCARPIA-ITMI Poster Analysis of the genomic structure of a wheat NIL population segregating for resistance to glume blotch with a 90k ILLUMINA SNP chip 29.06.2014 Wernigerode, Germany Sanchez Martin Javier;
EUCARPIA-ITMI Poster High-resolution analysis of a locus for resistance to wheat Stagonospora Nodorum Glume Blotch and evaluation of the impact of plant morphology in the disease resistance 29.06.2014 Wernigerode, Germany Sanchez Martin Javier;
CEPLAS Symposium 2014 Talk given at a conference Molecular breeding for improved fungal disease resistance in wheat 16.05.2014 Düsseldorf, Germany Keller Beat;
Food, Nutrition and Agriculture Genomics Congress (Oxford Global) Talk given at a conference Molecular basis of multiple pathogen resistance provided by expression of the wheat Lr34 gene 07.04.2014 London, Great Britain and Northern Ireland Keller Beat;
Meeting of the EU FP7 Project WHEALBI Talk given at a conference Allele and pathway mining for adaptive traits and grain quality 11.03.2014 Clermont Ferrand, France Keller Beat;
Translational Cereal Genomics Conference Talk given at a conference Molecular approaches towards durable resistance in cereals 11.02.2014 Vienna, Austria Keller Beat;
SWISSPLANT 2014 Talk given at a conference Molecular approaches to improve durable fungal disease resistance in wheat 29.01.2014 Meiringen, Switzerland Keller Beat;
Plant and Animal Genome XXI Meeting, Fungal Genomics Workshop Talk given at a conference Comparative genomics of wheat powdery mildew 13.01.2014 San Diego, United States of America Keller Beat;
20th ENGL Plenary Meeting, EU Commission Joint Research Centre, Institute for Health and Consumer Protection, Mol. Biology and Genomics Unit Talk given at a conference Transgene detection in wheat; a polyploid crop with large genome size 04.12.2013 Ispra, Italy Keller Beat;
PLANT FELLOWS Annual Meeting Talk given at a conference Molecular analysis of wheat disease resistance against glume blotch. 29.10.2013 Rigi Kulm, Switzerland Sanchez Martin Javier;
1st annual meeting COST FA 1208: Pathogen-informed strategies for sustainable broad-spectrum crop resistance. Talk given at a conference Transferability of the wheat Lr34 multi-pathogen resistance gene 09.10.2013 Birnam, Great Britain and Northern Ireland Sucher Justine;
1st annual meeting COST FA 1208: Pathogen-informed strategies for sustainable broad-spectrum crop resistance. Poster Hunting avirulence effectors in the wheat powdery mildew Blumeria graminis f.sp. tritici. 09.10.2013 Birnam, Great Britain and Northern Ireland Bourras Salim;
12th International Wheat Genetics Syposium Talk given at a conference Genomic approaches towards durable fungal disease resistance in wheat 13.09.2013 Yokohama, Japan Keller Beat;
6. Fachtagung zur Grünen Gentechnik, Plant Science Center, ETH Talk given at a conference Food for thought - Denkanstösse 06.09.2013 Zurich, Switzerland Keller Beat;
3rd international powdery mildew workshop Talk given at a conference Hunting avirulence genes in the wheat powdery mildew Blumeria graminis f.sp. tritici 29.08.2013 Copenhagen, Denmark Bourras Salim;
Annual Main Meeting of the Society of Experimental Biology Talk given at a conference From gene banks to the field: the transgenic use of cloned resistance genes 05.07.2013 Valencia, Spain Keller Beat;
Sommertagung 2013 der Gesellschaft für Pflanzenzüchtung, GFP; Justus-Liebig-Universität Talk given at a conference Genomforschung für und zu Weizen 25.06.2013 Giessen, Germany Keller Beat;
Plant Genomics Congress Talk given at a conference Evolutionary genomics of the wheat - powdery mildew pathosystem 14.05.2013 London, Great Britain and Northern Ireland Keller Beat;
Plant and Animal Genome XX Meeting, San Diego, USA; January 13 Talk given at a conference Breeding for fungal disease resistance - the of R genes as transgenes in wheat 13.01.2013 San Diego, United States of America Keller Beat;


Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Aargauische Naturforschende Gesellschaft, ANG Vorträge, Naturama Talk 25.02.2015 Aarau, Switzerland Keller Beat;
Informationsevent fon Euresearch, Universität Zürich, Talk 23.10.2013 Zurich, Switzerland Keller Beat;
Forschen in der Schweiz - Möglichkeiten und Schwierigkeiten, Verein Forschung für Leben Talk 10.10.2013 Bern, Switzerland Keller Beat;
Eidg. Fachkommission für biologische Sicherheit (EFBS) Talk 12.03.2013 Bern, Switzerland Keller Beat;


Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions The GMO debate: global, local and scientific considerations German-speaking Switzerland 2015
Talks/events/exhibitions Von alten und neuen Getreidearten: Interaktion von Mensch und Landwirtschaft German-speaking Switzerland 2015
Talks/events/exhibitions Genome Sequencing of Wheat German-speaking Switzerland 2014
Talks/events/exhibitions Gentechnik in der Pflanzenzüchtung: Heutige Anwendungen und Zukunftsperspektiven German-speaking Switzerland 2014
Talks/events/exhibitions Gentechnisch veränderte Pflanzen: Forschung und Anwendungen auf globaler und lokaler Ebene German-speaking Switzerland 2014
Talks/events/exhibitions Gentechnisch veränderter Weizen mit verbesserter Krankheitsresistenz: Forschungsversuche im Schweize German-speaking Switzerland 2014
Talks/events/exhibitions Pflanzenzüchtung: Vergangenheit, Gegenwart und Zukunft German-speaking Switzerland 2013
Talks/events/exhibitions Podiumsdiskussion "Zukunft der Gentechnologie und ihre Rolle bei der Ernährungssicherheit" Studentis German-speaking Switzerland 2013

Awards

Title Year
IS-MPMI International Congress Travel Awards​​​​ The 2014 IS-MPMI XVI International Congress Travel Awards are were named in honor of Dr. Ko Shimamoto. Dr. Shimamoto made notable contributions to the fields of flowering and plant immunity that brought him respect and recognition worldwide. 2014

Patents

Title Date Number Inventor Owner
Plant resistant to helminthosporium turcicum 04.09.2013 WO2015032494-A2 ; WO2015032494-A3

Associated projects

Number Title Start Funding scheme
127061 Molecular analysis of both broad-spectrum and specific fungal disease resistance in wheat 01.01.2010 Project funding
164086 Imaging of Deep-Lying Tissues with Enhanced Resolution and Sensitivity to Study Plant Development 01.12.2015 R'EQUIP
163260 Molecular analysis of three different types of fungal disease resistance mechanisms in cereals 01.01.2016 Project funding

Abstract

The proposed work aims at understanding the molecular basis of fungal disease resistance in the crop plant wheat, including experimental approaches in genetically more tractable grass species such as rice and barley. In a first project, we want to investigate the molecular function of the durable Lr34 gene originating from wheat. This resistance gene is acting quantitatively and is effective against several fungal pathogen species in wheat. The molecular mechanism of this unique and valuable resistance remains unclear and will be investigated in detail. The Lr34 resistance gene is agronomically very useful and has remained active despite growth of cultivars containing this gene during decades and on large area. In a second set of subprojects, the wheat-powdery mildew pathosystem will be studied, integrating work on the pathogen as well as on the host species. This will involve the use of the complete genome sequence of powdery mildew which we have recently established, as well as the physical map based on BAC contigs. These new tools for whole genome approaches will be used to identify genetic determinants of the interaction on the pathogen side.
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