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Characterization of arabidopsis mutants altered in their ability to express BABA-induced resistance (BABA-IR) and priming

English title Characterization of Arabidopsis mutants altered in their ability to express BABA-induced resistance (BABA-IR) and priming
Applicant Mauch-Mani Brigitte
Number 120197
Funding scheme Project funding
Research institution Lab. de Biologie Moléculaire et Cellulaire Institut de Biologie Université de Neuchâtel
Institution of higher education University of Neuchatel - NE
Main discipline Botany
Start/End 01.05.2008 - 30.06.2012
Approved amount 399'000.00
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Keywords (6)

Induced resistance; beta-aminobutyric acid; priming; potentiation; resistance; Arabidopsis

Lay Summary (English)

Lead
Lay summary
Plants treated with the non-protein amino acid beta-aminobutyric acid (BABA) develop an enhanced defensive capacity against a large variety of biotic and abiotic stresses. The expression of this BABA-induced resistance (BABA-IR) coincides with a faster and stronger defense response following pathogen attack or abiotic stress. This phenomenon has been termed as priming or potentiation, the underlying mechanism though is unknown. Priming could be based on the accumulation of signalling proteins in an inactive form that would then be activated upon exposure to stress. Another possibility is the accumulation of transcription factors in primed plants leading to an enhanced defence gene transcription when this plant is stressed. A further idea suggests that priming might occurs at the epigenetic level via changes in chromatin structure. Based on the observation that high amounts of BABA induce sterility in Arabidopsis, we have isolated mutants from a population of T-DNA mutagenized Arabidopsis with impaired BABA-induced sterility (ibs) phenotype. In this proposal we plan to concentrate on mutants ibs1 and ibs6. The function of several IBS genes in the defense signaling network of Arabidopsis has been elucidated by testing their reactions towards different biotic and abiotic stresses at the phenotypic and molecular level. Our data so far demonstrate that BABA-IR against biotic and abiotic stresses is based on priming of distinct defense signaling mechanisms involving salicylic acid-, abscisic acid-, and phosphoinositide-dependent pathways and that callose deposition plays an important role in BABA-IR.We propose first to get a more complete picture concerning the role of callose and vesicle transport in BABA-IR by further characterizing the ibs6 mutant. Additionally, the role of IBS1in priming will be investigated. Protein partners directly interacting with the protein kinase IBS1 will be determined and characterized. Possible substrates for IBS1 will be sought for . We will be using microarray technology to obtain a detailed knowledge of IBS1-dependent gene expression. Finally, we plan to further pursue our recent discovery that IBS1 messenger RNA can be postranscriptionally regulated and investigate the implications for priming.The results of the described research will lead to a better understanding of the priming phenomenon in plants. Additionally, they might eventually help to devise new stragegies in agronomy since priming-inducing agents confer enhanced resistance against biotic and abiotic stresses with minimal inhibitory effects on commercially important traits.
Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Detection, characterization and quantification of salicylic acid conjugates in plant extracts by ESI tandem mass spectrometric techniques
Pastor V, Vicent C, Cerezo M, Mauch-Mani B, Dean J, Flors V (2012), Detection, characterization and quantification of salicylic acid conjugates in plant extracts by ESI tandem mass spectrometric techniques, in PLANT PHYSIOLOGY AND BIOCHEMISTRY, 53, 19-26.
Descendants of Primed Arabidopsis Plants Exhibit Resistance to Biotic Stress
Slaughter A, Daniel X, Flors V, Luna E, Hohn B, Mauch-Mani B (2012), Descendants of Primed Arabidopsis Plants Exhibit Resistance to Biotic Stress, in PLANT PHYSIOLOGY, 158(2), 835-843.
Callose Deposition: A Multifaceted Plant Defense Response
Luna E, Pastor V, Robert J, Flors V, Mauch-Mani B, Ton J (2011), Callose Deposition: A Multifaceted Plant Defense Response, in MOLECULAR PLANT-MICROBE INTERACTIONS, 24(2), 183-193.
Callose deposition: a multifaceted plant defense response.
Luna Estrella, Pastor Victoria, Robert Jérôme, Flors Victor, Mauch-Mani Brigitte, Ton Jurriaan (2011), Callose deposition: a multifaceted plant defense response., in Molecular plant-microbe interactions : MPMI, 24(2), 183-93.
Priming for enhanced defence responses by specific inhibition of the Arabidopsis response to coronatine.
Tsai Chia-Hong, Singh Prashant, Chen Ching-Wei, Thomas Jerome, Weber Johann, Mauch-Mani Brigitte, Zimmerli Laurent (2011), Priming for enhanced defence responses by specific inhibition of the Arabidopsis response to coronatine., in The Plant journal : for cell and molecular biology, 65(3), 469-79.
Priming for enhanced defence responses by specific inhibition of the Arabidopsis response to coronatine.
Tsai Chia-Hong, Singh Prashant, Chen Ching-Wei, Thomas Jerome, Weber Johann, Mauch-Mani Brigitte, Zimmerli Laurent (2011), Priming for enhanced defence responses by specific inhibition of the Arabidopsis response to coronatine., in The Plant journal : for cell and molecular biology, 65(3), 469-79.
Role of ABA and abiotic factors on PAMP-induced callose deposition
Estrella Luna Jérôme Robert Victor Flors Yuhua Zhang Jurriaan Ton and Brigitte Mauch-Mani (2010), Role of ABA and abiotic factors on PAMP-induced callose deposition, in Hani Antoun Tyler Avis Louise Brisson Danielle Prévost and Martin Trepanier (ed.), 1-7.
Role of Abscisic Acid in Disease Resistance
Flors Victor Ton Jurraiaan Mauch-Mani Brigitte (2009), Role of Abscisic Acid in Disease Resistance, in KEIKO YOSHIOKA and KAZUO SHINOZAKI (ed.), 1-22.
Absolute quantification of specific plant defence pathways and disease development of several microbial pathogens in Arabidopsis using real-time PCR
Boachon Benoît Robert Jérôme Daniel Xavier Mauch-Mani Brigitte (2009), Absolute quantification of specific plant defence pathways and disease development of several microbial pathogens in Arabidopsis using real-time PCR, in Yigal Elad Monika Maurhofer Christoph Keel Cesare Gessler Brion Duffy (ed.), 1-8.

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
SEB-Meeting 13.06.2012 Salzburg, Oestereich
PR-IR 11 13.09.2011 Neuchatel, Switzerland
Induced Resistance in Plants against Insects and Diseases 13.08.2009 Granada, Spain
14th MPMI congress 19.07.2009 Quebec, Canada
Phytopathologie 2009 08.06.2009 Lyon-Villeurbanne, France
Experimental Plant Sciences 13.04.2009 Lenteren, The Netherlands
• 9th International Congress of Plant Pathology 08.08.2008 Torino, Italy


Associated projects

Number Title Start Funding scheme
128031 Priming of heat and drought tolerance in potato 01.01.2010 SCOPES
160162 Beta-aminobutyric acid (BABA) as a plant-produced priming agent: Biosynthesis, regulation and perception 01.05.2015 Project funding
105884 Characterization of Arabidopsis mutants altered in their ability to express BABA-induced resistance (BABA-IR) and priming 01.02.2005 Project funding
140593 Priming plants for biotic and abiotic stress resistance:investigating possible mechanisms 01.05.2012 Project funding

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