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A nitrogen cooled NMR cryoprobe and a new console for the measurement of low concentrated samples

English title A nitrogen cooled NMR cryoprobe and a new console for the measurement of low concentrated samples
Applicant Furrer Julien
Number 205330
Funding scheme R'EQUIP
Research institution Departement für Chemie, Biochemie und Pharmazie Universität Bern
Institution of higher education University of Berne - BE
Main discipline Physical Chemistry
Start/End 01.01.2022 - 31.12.2022
Approved amount 290'790.00
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All Disciplines (5)

Discipline
Physical Chemistry
Inorganic Chemistry
Organic Chemistry
Molecular Biology
Medical Microbiology

Keywords (5)

NMR spectroscopy; fast measurement; low concentration; chemistry; structure determination

Lay Summary (French)

Lead
Achat d'un équipement de résonance magnétique nucléaire (RMN) constitué d'une console (électronique) et d'une sonde de mesure "cryosonde" refroidie à l'azote gazeux. Cet équipement est destiné à réaliser des expériences de RMN avec des échantillons peu concentrés et à utiliser les nouvelles fonctionnalités offertes par la console, telles que l'acquisition parallèle de différents noyaux.
Lay summary

Ce nouvel équipement RMN sera particulièrement utile à divers projets de notre Institut de Chimie, Biochimie et Pharmacie, mais aussi de notre Université, par exemple en parasitologie et en bactériologie. L'avantage principal de ce nouvel équipement est d'augmenter la sensibilité des expériences RMN, de pouvoir utiliser de petites quantités d'échantillons, ou de pouvoir mesurer des échantillons disponibles seulement en très petites quantités.
Nous envisageons de déterminer des structures de peptides, et surtout obtenir des informations sur des nouvelles métallo-mini enzymes, qui sont presque impossibles à obtenir avec un équipement RMN standard. Ces mini enzymes sont utilisés dans le domaine de la biocatalyse, comme alternative plus respectueuse par rapport aux catalyseurs classiques inorganiques contenant des métaux lourds. Toujours dans le domaine de la catalyse, primordial pour l'industrie afin de synthétiser des molécules avec des structures adéquates, cet équipement permettra l'étude de nouveaux composés bimétalliques contenant des métaux tels le magnésium et le zinc, alternative également plus respectueuse que les catalyseurs classiques. Nous envisageons aussi l’étude des noyaux difficiles à observer tels que 199Hg ou 207Pb, avec comme but d'obtenir des informations sur le type de complexes que forment ces noyaux toxiques dans le corps humain avec des molécules dites chélatantes telles que l'acide lipoïque ou le glutathione. Enfin, l'étude de capsules de polysaccharides de Streptococcus pneumonia, qui ne peuvent être obtenues qu'en quantité limitée, sera facilitée et permettra sans doute d'obtenir de nouvelles informations, primordiales pour mieux comprendre les streptocoques et prévenir les infections.  

Direct link to Lay Summary Last update: 17.11.2021

Responsible applicant and co-applicants

Project partner

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188573 Sustainable Synthesis and Catalysis Through Earth-Abundant Cooperative Bimetallics 01.03.2020 Project funding
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Abstract

This proposal aims at obtaining financial support for the purchase of a 500 MHz NMR AVANCE Neo® console together with a very performant Broadband Observe (BBO) Prodigy® N2 cooled cryoprobe devoted to perform NMR experiments with low concentrated samples and to use the new features offered by the Neo console, such as parallel acquisition of differ-ent nuclei. We anticipate that organic and inorganic chemists, but also medicinal chemists, pharmacists, as well as biochemists and biologists will benefit from this new equipment.As exemplified by the projects in this proposal, this new NMR equipment will be par-ticularly well-suited for various projects ranging from inorganic chemistry to bacteriology, with the possibility of increasing the sensitivity of the experiments and of using small quanti-ties as common point. Peptides, heteronuclei (2H, 199Hg), but also polysaccharide capsules of Streptococcus pneumonia and especially minienzymes are difficult to obtain using a standard NMR probehead. The higher sensitivity reached with this new Prodigy® cryoprobe and the new AVANCE Neo® console will be a determinant asset to develop further these researches. In addition, new opportunities will be offered by combining non-uniform sampling (NUS) techniques, aimed at reducing the measurement time of nD experiments without com-promise on the resolution, with the possibility of recording nuclei in parallel, for instance for monitoring reactions or evaluating the stabilities of metal complexes in aqueous media.This new console and Prodigy probehead will be installed in the laboratories of the NMR group of the Department of Chemistry and Biochemistry (Prof. Dr. J Furrer) and will be freely accessible to any researcher from the University that requires the use of this instru-ment for his/her research. The access to this new equipment will be also granted for users from foreign universities, at very advantageous fees.As such, for the investigators of this proposal, the requested equipment would open new fields to NMR spectroscopic research that are currently not feasible or at best hardly thinkable with the current equipment available. Owing to the broad variety of samples falling into the application field of this new NMR equipment dedicated to measure low-concentrated samples and insensitive nuclei, it is expected that a diversified interdisciplinary usership will be able to benefit from this new equipment. the equipment will enable us to keep up with modern state-of-the-art technologies, explore new applications fields and extend our competences.
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