Project

Back to overview

Integrated Microscope for Live-Cell and Single-Molecule Imaging

Applicant Rivera Fuentes Pablo Marcelo
Number 189631
Funding scheme R'EQUIP
Research institution Laboratorium für Organische Chemie ETH Zürich
Institution of higher education EPF Lausanne - EPFL
Main discipline Organic Chemistry
Start/End 01.01.2020 - 31.12.2020
Approved amount 300'000.00
Show all

All Disciplines (5)

Discipline
Organic Chemistry
Cellular Biology, Cytology
Molecular Biology
Biochemistry
Physical Chemistry

Keywords (5)

Live-cell Imaging; Chemical Biology; Probes; Optical Microscopy; Biophysics

Lay Summary (French)

Lead
La découverte biologique actuelle repose sur l'observation directe des détails des structures subcellulaires. La microscopie optique est un outil essentiel pour permettre ces découvertes, de même que le développement de méthodes chimiques permettant d'obtenir un contraste dans les cellules vivantes. Dans le cadre de ce projet, un microscope doté d'une configuration unique sera acquis. Cet instrument permettra d'étudier de nombreux processus biologiques et de développer de nouvelles méthodes pour visualiser la biologie en temps réel.
Lay summary

Contenu et objectifs des travaux de recherche

Plusieurs groupes de recherche de l'EPFL profiteront de cet instrument. Parmi les nombreuses applications envisagées pour cet instrument, les suivantes sont mises en évidence :

- Observation des protéines dans le noyau des cellules humaines pour comprendre les mécanismes du maintien génétique.
- Détermination de l'absorption des nanoparticules par les bactéries.
- Etude du mécanisme d'action des nouveaux traitements contre le cancer.
- Suivi de la dynamique de l'ADN et des protéines associées à l'ADN dans la cellule.
- Observation d'enzymes actives dans des cellules vivantes avec résolution à molécule unique.

Tous ces projets visent en fin de compte à mieux comprendre les mécanismes des pathologies humaines pour le développement de thérapies améliorées.

Contexte social et scientifique du travail de recherche

Ce projet est principalement orienté vers la découverte des aspects fondamentaux de la chimie biologique. Cette compréhension plus approfondie des mécanismes chimiques à la base de la biologie cellulaire est une étape essentielle vers le développement de meilleures thérapies, avec une efficacité accrue et moins d'effets secondaires

Direct link to Lay Summary Last update: 20.11.2019

Responsible applicant and co-applicants

Project partner

Associated projects

Number Title Start Funding scheme
154269 Recombinant, Photosynthetic Bacteria for Photovoltaic Solar Energy Conversion 01.09.2015 Assistant Professor (AP) Energy Grants
182972 Genetic engineering of microbes and regulation of charge transfer dynamics for high performance biophotovoltaics 01.12.2019 India
180541 NCCR AntiResist (phase I) 01.08.2020 National Centres of Competence in Research (NCCRs)
165551 Development of Intracellular Photoactivatable Probes 01.04.2016 Project funding

Abstract

Optical microscopy plays an essential role in biological research. Through the development of novel probes and labels for optical microscopy, chemical biologists are enabling the visualization and control of biochemical processes in living organisms with increasing precision. The Institute of Chemical Sciences and Engineering (ISIC) of École Polytechnique Fédéral de Lausanne (EPFL) has recently strengthened its position as a global leader in chemical biology with a series of senior and junior hires. Many of these research groups develop innovative chemical probes for biological investigations using optical microscopy. This research is evolving at a pace that has surpassed the capacity of current optical microscopy resources at ISIC. This widening gap between research and infrastructure risks the development of innovative science. In this proposal, we request funds to co-finance the acquisition of a state-of-the-art optical microscope to close this gap. This instrument would combine live-cell, fast confocal imaging, single-molecule detection and tracking, and photoactivation capabilities. This equipment would serve a wide range of projects, ranging from the detection of single molecules to the elucidation of signaling pathways. The acquisition of this microscope would therefore address the needs of the growing chemical biology community at ISIC-EPFL for years to come. Importantly, this instrument would enable the execution of experiments that are not possible using currently available instrumentation at EPFL. Some of the specific research groups and projects that would benefit directly from the acquisition of this instrument are listed below:-Observing choreography of nuclear RNR-alpha in DNA replication and damage response (Aye)-Nanoparticle uptake and imaging in autofluorescent cyanobacteria (Boghossian)-Application of single-molecule imaging in live cells to facilitate the development of innovative treatments for mesothelioma (Dyson)-Imaging structure and dynamics of chromatin domains in cells (Fierz)-Development of sensors and probes for super-resolution optical microscopy and control of intracellular redox environments with photoactivatable probes (Rivera-Fuentes)In addition, an agreement has been reached with EPFL’s Bioimaging and Optics Platform (BIOP) to maintain and administer this microscope. BIOP has the personnel and expertise to ensure that the equipment operates efficiently and junior researchers are properly trained in its usage. Whereas the microscope would be primarily used by chemical biology labs at ISIC, through BIOP, this unique instrument would be shared beyond ISIC with the broader community at EPFL, allowing multiple research groups to perform experiments that are not possible with current BIOP instrumentation.
-