Organic Chemistry; Microbiology; Burkholderia; Natural Products
Sieber Simon, Mathew Anugraha, Jenul Christian, Kohler Tobias, Bär Max, Carrión Víctor J., Cazorla Francisco M., Stalder Urs, Hsieh Ya-Chu, Bigler Laurent, Eberl Leo, Gademann Karl (2021), Mitigation of Pseudomonas syringae virulence by signal inactivation, in Science Advances
, 7(37), eabg2293.
Sieber Simon, Hsiao Chien-Chi, Emmanouilidou Despina, Debowski Aleksandra W., Stubbs Keith A., Gademann Karl (2020), Syntheses and biological investigations of kirkamide and oseltamivir hybrid derivatives, in Tetrahedron
, 76(51), 131386-131386.
Sieber Simon, Daeppen Christophe, Jenul Christian, Mannancherril Vidya, Eberl Leo, Gademann Karl (2020), Biosynthesis and Structure–Activity Relationship Investigations of the Diazeniumdiolate Antifungal Agent Fragin, in ChemBioChem
, 21(11), 1587-1592.
Sieber Simon, Grendelmeier Simone M., Harris Lonnie A., Mitchell Douglas A., Gademann Karl (2020), Microviridin 1777: A Toxic Chymotrypsin Inhibitor Discovered by a Metabologenomic Approach, in Journal of Natural Products
, 83(2), 438-446.
||Sieber, Simon; Grendelmeier, Simone M.; Harris, Lonnie A.; Mitchell, Doug A.; Gademann, Karl
|Persistent Identifier (PID)
LOCUS NZ_SRLN01000000 4967080 bp DNA linear BCT 29-OCT-2020DEFINITION Microcystis aeruginosa EAWAG127a, whole genome shotgun sequencing project.ACCESSION NZ_SRLN00000000VERSION NZ_SRLN00000000.1DBLINK BioProject: PRJNA224116 BioSample: SAMN10998537 Assembly: GCF_008757435.1KEYWORDS WGS; RefSeq.SOURCE Microcystis aeruginosa EAWAG127a ORGANISM Microcystis aeruginosa EAWAG127a Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Microcystaceae; Microcystis.REFERENCE 1 (bases 1 to 4967080) AUTHORS Sieber,S., Grendelmeier,S.M., Harris,L.A., Mitchell,D.A. and Gademann,K. TITLE Microviridin 1777: A Toxic Chymotrypsin Inhibitor Discovered by a Metabologenomic Approach JOURNAL J Nat Prod 83 (2), 438-446 (2020) PUBMED 31989826REFERENCE 2 (bases 1 to 4967080) AUTHORS Grendelmeier,S.M., Sieber,S. and Gademann,K. TITLE Direct Submission JOURNAL Submitted (03-APR-2019) Chemistry, University of Zurich, Winterthurerstrasse 190, Zurich 8050, SwitzerlandCOMMENT REFSEQ INFORMATION: The reference sequence is identical to SRLN00000000.1. The Microcystis aeruginosa EAWAG127a whole genome shotgun (WGS) project has the project accession NZ_SRLN00000000. This version of the project (01) has the accession number NZ_SRLN01000000, and consists of sequences SRLN01000001-SRLN01000022. Bacteria and source DNA available from Cyanobacteria Collection at University of Zurich. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: JUL-2018 Assembly Method :: HGAP v. 2.3.0 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 63.0x Sequencing Technology :: PacBio RSII ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Date :: 10/29/2020 04:20:22 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 4.13 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 4,779 CDSs (total) :: 4,722 Genes (coding) :: 4,214 CDSs (with protein) :: 4,214 Genes (RNA) :: 57 rRNAs :: 2, 2, 2 (5S, 16S, 23S) complete rRNAs :: 2, 2, 2 (5S, 16S, 23S) tRNAs :: 46 ncRNAs :: 5 Pseudo Genes (total) :: 508 CDSs (without protein) :: 508 Pseudo Genes (ambiguous residues) :: 0 of 508 Pseudo Genes (frameshifted) :: 227 of 508 Pseudo Genes (incomplete) :: 250 of 508 Pseudo Genes (internal stop) :: 146 of 508 Pseudo Genes (multiple problems) :: 105 of 508 CRISPR Arrays :: 6 ##Genome-Annotation-Data-END##FEATURES Location/Qualifiers source 1..4967080 /organism="Microcystis aeruginosa EAWAG127a" /mol_type="genomic DNA" /strain="EAWAG127a" /isolation_source="Mazurian Lakes" /db_xref="taxon:2529855" /country="Poland" /collection_date="1965" /collected_by="Alfons Zehnder"WGS SRLN01000001-SRLN01000022WGS_SCAFLD NZ_SRLN01000001-NZ_SRLN01000022
Bacteria use chemical compounds for cell-cell communication. This process is of relevance for various biological processes such as virulence, biofilm formation, or luminescence, with ramifications to different fields such as human health, crop protection, and industrial biotechnology. Although a number of communication systems have been established and characterized over the last years, their number has remained surprisingly low. In collaboration of the Eberl and Gademann groups, we have identified this year a new class of signal molecules containing a diazenium diolate group.The ultimate goal of this proposal is to provide a detailed understanding of this new class of signaling molecules in bacteria, with regard to their biosynthesis, their biological function, their mechanism of action and their role in pathogenesis. Specifically, we aim to investigate (1) the structure, distribution, and biological activities of diazenium diolate-based signal molecules, (2) the signal perception, downstream regulatory cascades, and the associated bacterial phenotypes, (3) their role of in metal homeostasis, and (4) the biosynthesis of these signal molecules.Akin to discovering a new language, this work will therefore unravel the complex molecular structures of a novel class of bacterial signals, their intricate mechanism of action, and their importance in the regulation of different phenotypic traits. Preliminary data suggest that this class of signaling molecules might be involved in society-relevant problems: From pathogenicity in plants and animals to beneficial biocontrol functions. Over the last ten years, the Eberl and Gademann research groups have established a close research collaboration that successfully investigated a number of scientific problems related to bacterial signaling or bacteria/plant symbiosis. The proposed research builds on this strong collaboration between two research groups in chemistry and microbiology, with a documented record of excellence as judged by outstanding joint publications, awards to PIs and students alike, and published comments by peers.We will pursue an interdisciplinary approach in which projects are thoroughly investigated by scientists with complementary expertise, graduate students will have joint PhD committees, and secondments in different labs will strengthen the transdisciplinary knowledge of the researchers. Joint teaching efforts on the master level will educate the next generation of students, and two international symposia will allow for dissemination of the science and fostering the community. Overall, this project leverages the complementary expertise of two leading groups in chemistry and biology with documented excellence in interdisciplinary research. The next generation of scientists will work jointly on all projects and will be educated to tackle the transdisciplinary challenges of the next decades. The strong consortium will investigate a novel chemical language in bacteria, which has the potential to impact science and society.