multidimensional single cell analysis; mass cytometry; Hyperion
Dommann Noëlle, Sánchez-Taltavull Daniel, Eggs Linda, Birrer Fabienne, Brodie Tess, Salm Lilian, Baier Felix Alexander, Medová Michaela, Humbert Magali, Tschan Mario P., Beldi Guido, Candinas Daniel, Stroka Deborah (2020), The LIM Protein Ajuba Augments Tumor Metastasis in Colon Cancer, in Cancers
, 12(7), 1913-1913.
Complex diseases commonly involve large numbers of genetic or epigenetic changes across several cell types, each of variable pathologic and therapeutic relevance. Identifying and understanding how such alterations collectively drive cellular dysfunction is a mandatory requirement in the implementation of precision medicine and requires complex technological efforts along with state-of-the-art computational tools. In that respect, single-cell technologies are entering the mainstream, providing an important utility to elucidate a diverse range of complex biological phenomena. Moreover, single-cell measurements are routinely becoming indispensable for the study of disease biology since heterogeneous cell populations undergo dynamic changes, differentiating toward many distinct identities a fact that has a key role in adjusting the appropriate therapies. This project will install a Hyperion CyTOF Imaging System that will enable comprehensive analysis of cellular phenotypes and their interrelationships through Imaging Mass Cytometry. This platform will be used to measure multiple protein markers in the spatial context of the tissue microenvironment and in single cell suspensions, gain full microenvironment and tissue architecture data, run panels of over 40 detection parameters, and avoid non-specific signals obtained from fluorescence reporter overlap.This new platform instrument will provide the researchers at the University of Bern with a next generation state-of-the-art tool to study complex disease biology and to delve into intercellular communication with a higher degree of combined chemical and spatial information.