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CLE peptides-unraveling the functions in root growth and adaptations

Applicant Hazak Ora
Number 179745
Funding scheme Ambizione
Research institution Département de Biologie Faculté des Sciences Université de Fribourg
Institution of higher education University of Fribourg - FR
Main discipline Molecular Biology
Start/End 01.03.2019 - 28.02.2023
Approved amount 1'006'833.00
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Keywords (4)

root development; CLE peptides; root adaptations; BAM receptor-like kinases

Lay Summary (German)

Lead
Auf der Suche nach neuen Signalwegen bei der Anpassung der Anlage an sich verändernde Umgebungen
Lay summary

Um Wachstum und Reaktionen auf die Umwelt zu koordinieren müssen mehrzellige Organismen die Herausforderung, eine effiziente Kommunikation zwischen verschiedenen Organen und Geweben aufrechtzuerhalten, bewältigen. Sowohl Pflanzen als auch Tiere produzieren biologisch aktive Verbindungen, wie z. B. kleine Peptide, die sich zwischen den Zellen durch das Gefäßsystem oder den interzellulären Raum bewegen und eine Vielzahl von Entwicklungsprozessen und Stressreaktionen regulieren. In Pflanzen steuern, zusätzlich zu den primären Hormonen, eine Vielzahl von sekretierten Peptiden (mehr als 1000 in Arabidopsis) optimales Wachstum und Anpassung an Umweltveränderungen. Bemerkenswerterweise entwickelten Pflanzen eine große Anzahl von leucinreichen Receptor-like-Kinases (LRR-RLKs), um verschiedene Liganden zu erfassen, von durch Zellwandschäden induzierten Polysacchariden bis zu Steroidhormonen und den genannten ausgeschiedenen Peptiden.

Das hier vorgeschlagenen Projekt konzentriert sich auf die Aufklärung der Funktionen der CLAVATA3 / EMBRYO SURROUNDING REGION (CLE) -Peptide und ihrer jeweiligen Rezeptoren in der Kontrolle des Wurzelwachstums. Wir planen die Rolle von CLE-Peptiden bei der Bildung von wasserleitendem Gewebe (Xylem) sowie die von molekularen Mechanismen der CLE-Peptid-abhängigen Wurzeladaptionen zu untersuchen. Um ein besseres Verständnis der biologischen Funktionen dieser Peptide zu ermöglichen, möchten wir außerdem neue Werkzeuge entwickeln, um die Vorläufer und reifen CLE-Peptide in planta zu markieren und zu verfolgen. In Zusammenarbeit mit Laboratorien für strukturelle Biologie und organische Chemie planen wir eine Kombination aus genetischen Ansätzen, molekularem Design, organischer Synthese, Zellbiologie, Biochemie, hochauflösender Konfokalmikroskopie, Hochdurchsatzanalyse von genspezifischen Expressionsmustern und pflanzenphysiologischen Untersuchungsmethoden zu verwenden. Wir wollen die Expressionsmuster verschiedener CLE-Peptide in verschiedenen Wurzelgeweben kartieren und die Aktivitäten der CLE-Promotoren unter Stressbedingungen wie Hitze, Kälte, Nährstoffverfügbarkeit und hohen Salzkonzentrationen analysieren. Die Ergebnisse dieser Arbeit werden Aufschluss über die biologische Bedeutung von CLE-Peptiden und ihrer Rezeptoren geben und zu einem besseren Verständnis der Anpassungsmechanismen der Wurzel unter Stress führen.

Direct link to Lay Summary Last update: 12.02.2019

Lay Summary (English)

Lead
Discovering new signaling pathways in plant adaptation to changing environment
Lay summary

Multicellular organisms cope with the challenge of maintaining efficient communication between different organs and tissues to coordinate growth and responses to the environment. Both, plants and animals produce biologically active compounds such as small peptides that move between cells through the vascular system or intercellular spaces and regulate plethora of developmental processes and stress responses. In plants, in addition to major, a myriad of secreted peptides (more than 1000 in Arabidopsis) orchestrate optimal growth and adaptation to environmental changes. Remarkably, plants evolved a large number of leucine-rich repeat receptor-like kinases (LRR-RLKs) to sense a wide range of ligands, from cell wall damage-induced polysaccharides, to steroid hormones and secreted peptides. This proposal focuses on unraveling the functions of the CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides and their cognate receptors in the control of root growth. We plan to explore the role of CLE peptides in the formation of water conductive tissue called xylem, as well as to investigate the molecular mechanisms of CLE-peptides-dependent root adaptations. In addition, to allow for a better understanding of the biological functions of these peptides, we aim to develop new tools to tag and track the precursors and mature CLE peptides in planta. In part in collaboration with structural biology and organic chemistry laboratories, we will use a combination of genetic approaches, molecular design, organic synthesis, cell biology, biochemistry, high-resolution confocal microscopy, high throughput analysis of gene specific expression patterns, and plant physiology assays. We will map the expression patterns of different CLE peptides in different root tissues and screen CLE promoters’ activities under stress conditions including heat, cold, nutrient availability, and high salt conditions. The outcomes of this work will shed light on the biological importance of CLE peptides and their receptors as well as lead to a better understanding of mechanisms of root stress adaptations.

Direct link to Lay Summary Last update: 12.02.2019

Responsible applicant and co-applicants

Employees

Collaboration

Group / person Country
Types of collaboration
Weistain Lab/Tel Aviv University Israel (Asia)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Hothorn Lab/University of Geneva Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Gerber Sabina Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Plant Peptides and Receptors Meeting 2020 Talk given at a conference Decoding the Peptide-Receptor Pathways in Xylem Formation and Adaptation to Water Deficit 04.09.2020 Mont Perelin, Switzerland Hazak Ora;
Invited Plant Biology Seminar for the University of Montpellier, France Individual talk Role of Plant Receptors in Root Growth and Adaptations 07.05.2020 Montpellier, France Hazak Ora;
Annual Swiss Plant Meeting 2020 Poster CLE peptides in rot growth and adaptations 31.01.2020 Ovronnaz, Switzerland Hazak Ora;
European Plant Vascular Meeting 2019 Talk given at a conference Shaping Xylem Capillaries through the Receptor Peptide modules 20.11.2019 Corsendonk, Belgium Hazak Ora;
Plant Peptides and Receptors meeting 2019 Poster Building a high way through the Receptor-Peptide pathways 11.09.2019 Freudenstadt, Germany Hazak Ora;
Invited Biology Seminar at the University of Neuchatel Individual talk Root Biology 09.05.2019 University of Neuchatel, Switzerland Hazak Ora;


Associated projects

Number Title Start Funding scheme
189892 Designing Stress-Resistant Crops by Modulating Receptor-Peptide-Dependent Pathways 01.03.2020 COST (European Cooperation in Science and Technology)

Abstract

Multicellular organisms cope with the challenge of maintaining efficient communication between different organs and tissues to coordinate growth and responses to the environment. Both, plants and animals produce biologically active compounds such as small peptides that move between cells through the vascular system or intercellular spaces and regulate plethora of developmental processes and stress responses. In plants, in addition to major, a myriad of secreted peptides (more than 1000 in Arabidopsis) orchestrate optimal growth and adaptation to environmental changes. Remarkably, plants evolved a large number of leucine-rich repeat receptor-like kinases (LRR-RLKs) to sense a wide range of ligands, from cell wall damage-induced polysaccharides, to steroid hormones and secreted peptides. This proposal focuses on unraveling the functions of the CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides and their cognate receptors in the control of root growth. We plan to explore the role of CLE peptides in the formation of water conductive tissue called xylem, as well as to investigate the molecular mechanisms of CLE-peptides-dependent root adaptations. In addition, to allow for a better understanding of the biological functions of these peptides, we aim to develop new tools to tag and track the precursors and mature CLE peptides in planta. In part in collaboration with structural biology and organic chemistry laboratories, we will use a combination of genetic approaches, molecular design, organic synthesis, cell biology, biochemistry, high-resolution confocal microscopy, high throughput analysis of gene specific expression patterns, and plant physiology assays. We will map the expression patterns of different CLE peptides in different root tissues and screen CLE promoters’ activities under stress conditions including heat, cold, nutrient availability, and high salt conditions. The outcomes of this work will shed light on the biological importance of CLE peptides and their receptors as well as lead to a better understanding of mechanisms of root stress adaptations.
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