Project

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High throughput, Automated Cell Multi-Mode Imaging Reader for the University of Fribourg

English title High throughput, Automated Cell Multi-Mode Imaging Reader for the University of Fribourg
Applicant Weisskopf Laure
Number 177093
Funding scheme R'EQUIP
Research institution Département de Biologie Faculté des Sciences Université de Fribourg
Institution of higher education University of Fribourg - FR
Main discipline Cellular Biology, Cytology
Start/End 01.12.2017 - 30.11.2018
Approved amount 99'933.00
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All Disciplines (5)

Discipline
Cellular Biology, Cytology
Embryology, Developmental Biology
Molecular Biology
Experimental Microbiology
Biochemistry

Keywords (11)

Animal development; Multimode imaging reader; BRET; Phytophthora infestans; Cancer stem cells; Encephalitis; Auxin; Zebrafish; Cytation; Phytophatology; Bacterial volatiles

Lay Summary (French)

Lead
L’utilisation de marqueurs fluorescents ou luminescents pour suivre et comprendre les processus biologiques au niveau cellulaire ou subcellulaire est un procédé de plus en plus répandu dans les sciences de la vie. Dans la plupart des instruments, il faut choisir entre une analyse quantitative (quantité de lumière absorbée ou émise par les marqueurs) et qualitative (image de la cellule ou du compartiment subcellulaire sans quantification). L’instrument acquis grâce à ce subside du FNS permettra aux chercheurs en biologie et en médecine de l’Université de Fribourg de combiner ces deux approches pour étudier des thématiques aussi variées que les cellules cancéreuses, les maladies virales, le développement animal et végétal ou encore les interactions entre micro-organismes.
Lay summary

La possibilité de quantifier et d’imager des échantillons dans un système à haut débit permettant une gestion automatisée du matériel à analyser, de son incubation à son analyse, ouvrira des perspectives significatives dans la recherche en biologie et en médecine. Plus particulièrement, l’instrument en question (Cytation5 de la firme Biotek) permettra une grande diversité de détection optique (absorbance, fluorescence, luminescence), d’imagerie (différents objectifs) et de types d’échantillons (microplaques, boîtes de Petri ou lames de microscopie) tout en offrant une automatisation des processus d’insertion, d’incubation, de lecture et d’imagerie. Cette versatilité permettra de satisfaire les besoins de nombreux chercheurs de l’Université de Fribourg et des environs et de contribuer à une meilleure compréhension des processus biologiques menant par exemple à la formation de cellules cancéreuses, à la propagation de maladies virales à encéphalites, à la croissance et au développement des plantes, à la régénération des organes chez les animaux ou encore à l’activité antifongique de bactéries isolées du microbiome des plantes.

Direct link to Lay Summary Last update: 29.11.2017

Responsible applicant and co-applicants

Publications

Publication
Combining Different Potato-Associated Pseudomonas Strains for Improved Biocontrol of Phytophthora infestans
De Vrieze Mout, Germanier Fanny, Vuille Nicolas, Weisskopf Laure (2018), Combining Different Potato-Associated Pseudomonas Strains for Improved Biocontrol of Phytophthora infestans, in Frontiers in Microbiology, 9, 2573-2573.

Associated projects

Number Title Start Funding scheme
159995 Mechanisms of organ regeneration in zebrafish 01.10.2015 Project funding

Abstract

New analytical developments in recent years have given rise to high-throughput, multi-well analysis techniques usually based on quantitative measurement of absorbance, fluorescence or luminescence. In parallel, imaging phenotypes at the cellular or sub-cellular level using e.g. fluorescent markers represents a powerful tool for the detailed understanding of a multitude of biological processes. Combining the two aspects - quantitative (reading) and qualitative (imaging) - in a high throughput manner is now possible thanks to the development of multi-mode imaging readers, such as the Cytation 5 system (Biotek) applied for in this proposal. The combination of automated plate feeding, incubating, reagent dispensing, absorbance/fluorescence/luminescence reading and of microscopic imaging in fluorescent, brightfield and color brightfield modes makes this instrument a highly powerful and versatile tool for diverse research applications. In this proposal, five projects spanning from cancer stem cell research, encephalitis viruses, animal and plant development to bacteria-oomycete interactions detail their need for a multi-mode imagine reader and describe the scientific significance and outcome of the research such an instrument would enable. These projects all rely on the combination of quantitative and imaging data collection, some using solely the fluorescent or the luminescence mode, others a combination of brightfield and fluorescence modes. Beyond these five projects, for which the need of the multi-mode imaging reader was most pressing, the proposal also lists a high number of potential users from the medicine, biology and chemistry departments.The Cytation 5 system from Biotek does not have any true competitor on the market, as detailed in the application, which highlights the added value of this instrument compared to similar machines offering only some of its features. Earlier versions (Cytation 3) are available in other Swiss universities and research institutions; however, the configuration needed for the applicants of this proposal and for the broader community of interested scientists from our University is not yet available in any Swiss University. Moreover, prospective usage inquiries indicate that the instrument will be used intensively enough to deserve on site installation.This is a joint application from the Department of Medicine and the Department of Biology of the University of Fribourg. These two departments already share many pieces of equipment, some of which are commonly managed in a light microscopy platform (Bioimage Unifr). The object of the present application, the Cytation 5 multi-mode cell imaging reader, would be integrated into a shared analytical platform and made available to a wide range of researchers from our University and from neighboring institutions.
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