anthrax; DHHC; palmitoylation; CMG2; Acyl Protein thioesterase; collagen VI
Abrami Laurence, Audagnotto Martina, Ho Sylvia, Marcaida Maria Jose, Mesquita Francisco S., Anwar Muhammad U., Sandoz Patrick A., Fonti Giulia, Pojer Florence, Dal Peraro Matteo, van der Goot F. Gisou (2021), Palmitoylated acyl protein thioesterase APT2 deforms membranes to extract substrate acyl chains, in Nature Chemical Biology
, 17(4), 438-447.
PiotNuma, van der GootGisou F, SergeevaOksana A (2021), Harnessing the Membrane Translocation Properties of AB Toxins for Therapeutic Applications, in Toxins (Basel)
CastanonIrinka, HannichJ Thomas, AbramiLaurence, HuberFlorian, DuboisM, MullerM, van der GootGisou F, Gonzalez-GaitanMarcos (2020), Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafish, in Nature Communications
BürgiJérôme, AbramiLaurence, CastanonIrinka, AbriataLuciano A, KunzBéatrice, YanShixu Emili, LeraManuel, UngerSheila, Superti-FurgaAndrea, Dal PeraroMatteo, Gonzalez GaitanMarcos, van der GootGisou F (2020), Ligand Binding to the Collagen VI Receptor Triggers a Talin-to-RhoA Switch that Regulates Receptor Endocytosis, in Developmental Cell
Sarmento MesquitaFrancisco, van der GootGisou F, SergeevaOksana A (2020), Mammalian membrane trafficking as seen through the lens of bacterial toxins, in Cellular Microbiology
GadallaMohamed Rasheed, AbramiLaurence, van der GootGisou F, VeitMichael (2020), Hemagglutinin of Influenza A, but not of Influenza B and C viruses is acylated by ZDHHC2, 8, 15 and 20, in Biochem J
SandozPatrick A, TremblayChristopher, van der GootGisou F, FrechinMathieu (2019), Image-based analysis of living mammalian cells using label-free 3D refractive index maps reveals new organelle dynamics and dry mass flux, in PLoS Biol
SergeevaOksana A, van der GootGisou F (2019), Converging physiological roles of the anthrax toxin receptors, in F1000Research
AbramiLaurence, Denhardt-ErikssonRobin A, HatzimanikatisVassily, van der GootGisou F (2019), Dynamic Radiolabeling of S-Palmitoylated Proteins, in Linder Maurine E (ed.), Humana, New York, 111.
BlancMathieu, DavidFabrice PA, van der GootGisou F (2019), SwissPalm 2: Protein S-Palmitoylation Database, in Linder Maurine E (ed.), Humana, New York, 203.
CressiotBenjamin, OuldaliHadjer, Pastoriza-GallegoManuela, BacriLaurent, van der GootGisou F, PeltaJuan (2019), Aerolysin, a Powerful Protein Sensor for Fundamental Studies and Development of Upcoming Applications, in ACS Sensors
SergeevaOksana A, van der GootGisou F (2019), Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes, in Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes
GouttenoireJérôme, Pollán Angela, AbramiLaurence, Oechslin Noémie, MauronJohann, MatterMaxime, OppligerJoël, Szkolnicka Dagmara, Dao ThiViet Loan, van der GootGisou F, MoradpourDarius (2018), Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion, in PLoS Pathogens
ZaballaMaria Eugenia, van der GootGisou F (2018), The molecular era of protein S-acylation: spotlight on structure, mechanisms, and dynamics, in Crit Rev Biochem Mol Biol
HaagSimone M, GulenMuhammet F, ReymondLuc, GibelinAntoine, AbramiLaurence, DecoutAlexiane, HeymannMichael, van der GootGisou F, TurcattiGerardo, Behrendt Rayk, AblasserAndrea (2018), Targeting STING with covalent small-molecule inhibitors, in Nature
Kathayat Rahul S, CaoYang, ElviraPablo D, SandozPatrick A, ZaballaMaria-Eugenia, SpringerMaya Z, DrakeLauren E, MacleodKay F, van der GootGisou F, DickinsonBryan C (2018), Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes, in Nature Communications
During the upcoming granting period, we will pursue the two research axes of the 2014 proposal, namely 1) study of the physiological and pathological roles of anthrax toxin receptors, and 2) seek a better understanding of S-palmitoylation in the context of anthrax toxin receptors but also more generally.Anthrax toxin receptors. We will finalize and validate our screens on exosome biogenesis and uptake using anthrax toxin as a tool (not further described here) and pursue our studies on the molecular requirements of anthrax toxin signaling and trafficking. We will also launch a new research avenue that builds on our recent finding that the anthrax toxin receptor, CMG2, is a receptor for Collagen VI. We will investigate the role of CMG2 in fat metabolism and in muscle regeneration, since collagen VI has been shown to play an important role in these processes.S-palmitoylation. CMG2 undergoes palmitoylation. Preliminary experiments indicate that this is important for CMG2 folding in the endoplasmic reticulum, residence time at the cell surface and signaling capacity. We will investigate these processes in detail, study the dynamics of palmitoylation-depalmitoylation and examine how these processes are regulated. To do so, as successfully done in the past, we will combine a vast panel of approaches including in vitro structural and enzymatic analyses, cell-based functional and morphological assays, animal experimentation and mathematical modeling.