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Plant membrane receptor kinase complexes and their connection to cytoplasmic signaling cascades

English title Plant membrane receptor kinase complexes and their connection to cytoplasmic signaling cascades
Applicant Hothorn Michael
Number 176237
Funding scheme Project funding
Research institution Département de Biologie Végétale Faculté des Sciences Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Botany
Start/End 01.10.2017 - 30.09.2021
Approved amount 1'112'000.00
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All Disciplines (2)

Discipline
Botany
Biochemistry

Keywords (8)

arabidopsis genetics; Peptide signaling; Signal transduction; Protein crystallography; plant development; Brassinosteroid signaling; plant biochemistry; Receptor kinase

Lay Summary (German)

Lead
Pflanzen besitzen genau wie Tiere Hormone, welche das Wachstum und die Entwicklung des Organismus steuern und koordinieren. Diese Hormone werden an der Zelloberfläche erkannt und steuern die Genexpression im Zellkern. Das vorliegende Projekt versucht zu verstehen, wie verschiedene Hormone von der Zelle erkannt werden und wie die Signalleitung von der Zelloberfläche bis in den Zellkern funktioniert.
Lay summary

Mehrzellige Lebewesen koordinieren die Teilung und das Wachstum ihrer Zellen um zu wachsen und um verschiedene Gewebe und Organe zu bilden. Pflanzen benutzen ähnlich wie Tiere kleine Signalmoleküle (Hormone), die von einigen Zellen ausgeschieden werden und von anderen Zellen erkannt werden. In Pflanzen werden Hormone von bestimmten Rezeptoren an der Zelloberfläche gebunden. Der Rezeptor kann nach der Erkennung des Hormons ein Signal innerhalb der Zelle erzeugen, welches in den Kern wandern muss um dort ein bestimmtes genetisches Programm anzuschalten. Unser Projekt versucht zu verstehen, wie verschiedene Hormone von ihren Rezeptoren erkannt werden und wie das Erkennungssignal von der Zelloberfläche in den Zellkern transportiert wird. Dazu bilden mehrere Eiweißmoleküle eine Signalkette in der Informationen von einem Eiweiß zum nächsten weitergegeben werden. Wir möchten verstehen, wie diese Informationen kodiert sind und auf wie sie sich in der Zelle bewegen.

Unser Projekt ist Grundlagenforschung in der Pflanzenbiologie. Ein genaues Verständnis pflanzlicher Signalleitung könnte jedoch genutzt werden, das Wachstum und die Entwicklung von Nutzpflanzen gezielt zu beeinflussen.

Direct link to Lay Summary Last update: 29.09.2017

Responsible applicant and co-applicants

Employees

Publications

Publication
Constitutive Activation of Leucine-Rich Repeat Receptor Kinase Signaling Pathways by BAK1-Interacting Receptor-Like Kinase 3 Chimera
Hohmann Ulrich, Ramakrishna Priya, Wang Kai, Lorenzo-Orts Laura, Nicolet Joel, Henschen Agnes, Barberon Marie, Bayer Martin, Hothorn Michael (2020), Constitutive Activation of Leucine-Rich Repeat Receptor Kinase Signaling Pathways by BAK1-Interacting Receptor-Like Kinase 3 Chimera, in The Plant Cell, tpc.00138.-tpc.00138..
Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2
Okuda Satohiro, Fujita Satoshi, Moretti Andrea, Hohmann Ulrich, Doblas Verónica G., Ma Yan, Pfister Alexandre, Brandt Benjamin, Geldner Niko, Hothorn Michael (2020), Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2, in Proceedings of the National Academy of Sciences, 117(5), 2693-2703.
A two-way molecular dialogue between embryo and endosperm is required for seed development
Doll N. M., Royek S., Fujita S., Okuda S., Chamot S., Stintzi A., Widiez T., Hothorn M., Schaller A., Geldner N., Ingram G. (2020), A two-way molecular dialogue between embryo and endosperm is required for seed development, in Science, 367(6476), 431-435.
Mechanistic insights into the evolution of DUF26-containing proteins in land plants
Vaattovaara Aleksia, Brandt Benjamin, Rajaraman Sitaram, Safronov Omid, Veidenberg Andres, Luklová Markéta, Kangasjärvi Jaakko, Löytynoja Ari, Hothorn Michael, Salojärvi Jarkko, Wrzaczek Michael (2019), Mechanistic insights into the evolution of DUF26-containing proteins in land plants, in Communications Biology, 2(1), 56-56.
The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling
Hohmann Ulrich, Nicolet Joël, Moretti Andrea, Hothorn Ludwig A., Hothorn Michael (2018), The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling, in Nature Plants, 4(6), 345-351.
Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors
Hohmann Ulrich, Santiago Julia, Nicolet Joël, Olsson Vilde, Spiga Fabio M., Hothorn Ludwig A., Butenko Melinka A., Hothorn Michael (2018), Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors, in Proceedings of the National Academy of Sciences, 115(13), 3488-3493.

Datasets

Crystal structure of the LRR ectodomain of the plant membrane receptor kinase GASSHO1/SCHENGEN3 from Arabidopsis thaliana in complex with CASPARIAN STRIP INTEGRITY FACTOR 2

Author Okuda, Satohiro; Moretti, Andrea; Hothorn, Michael
Publication date 29.01.2020
Persistent Identifier (PID) https://www.rcsb.org/structure/6S6Q
Repository Protein Data Bank


Crystal structure of the BIR3 - SERK2 complex from Arabidopsis thaliana.

Author Hothorn, Michael; Hohmann, Ulrich
Publication date 04.04.2018
Persistent Identifier (PID) https://www.rcsb.org/structure/6G3W
Repository Protein Data Bank


Crystal structure of the BIR2 ectodomain from Arabidopsis thaliana.

Author Hothorn, Michael; Hohmann, Ulrich
Publication date 24.01.2018
Persistent Identifier (PID) https://www.rcsb.org/structure/6FG7
Repository Protein Data Bank


Crystal structure of the BIR3 - SERK1 complex from Arabidopsis thaliana.

Author Hothorn, Michael; Hohmann, Ulrich
Publication date 24.01.2018
Persistent Identifier (PID) https://www.rcsb.org/structure/6FG8
Repository Protein Data Bank


Crystal structure of the BRI1 Gly644-Asp (bri1-6) mutant from Arabidopsis thaliana.

Author Hothorn, Michael; Santiago, Julia; Hohmann, Ulrich
Publication date 31.01.2018
Persistent Identifier (PID) https://www.rcsb.org/structure/6FIF
Repository Protein Data Bank


Crystal structure of the tandem DUF26 ectodomain from the Arabidopsis thaliana cysteine-rich receptor-like protein PDLP5.

Author Brandt, Benjamin; Hothorn, Michael
Publication date 26.12.2018
Persistent Identifier (PID) https://www.rcsb.org/structure/6GRE
Repository Protein Data Bank


Crystal structure of the tandem DUF26 ectodomain from the Arabidopsis thaliana cysteine-rich receptor-like protein PDLP8.

Author Brandt, Benjamin; Hothorn, Michael
Publication date 19.12.2018
Persistent Identifier (PID) https://www.rcsb.org/structure/6GRF
Repository Protein Data Bank


Collaboration

Group / person Country
Types of collaboration
Dr. Michael Wrzaczek, University of Helsinki Finland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Niko Geldner, UNIL Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Melinka Butenko, University of Oslo Norway (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Dr. Gwyneth Ingram, ENS Lyon France (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Exchange of personnel

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
UZH/ETHZ BSM PhD retreat Morschach Talk given at a conference Plant signal transduction – from atoms to phenotypes and back 27.08.2019 Morschach, Switzerland Hothorn Michael;
19th HFSP awardees meeting Talk given at a conference Mechanistic insight into CIF peptides ligand binding and signaling activation in plant root development 10.07.2019 Tsukuba, Japan Okuda Satohiro;
PGSA meeting Paris Talk given at a conference Small molecule and peptide hormone sensing and signaling by membrane receptor kinases 26.06.2019 Paris, France Hothorn Michael;
Plant Science student conference Halle Talk given at a conference Mechanistic Insights into the Activation and Regulation of Plant Membrane Receptor Kinases 25.06.2019 Halle, Germany Hothorn Michael;
Symposium for Plant Receptor Kinases and Cell Signaling 2019 Talk given at a conference Mechanistic insight into CIF peptides binding on SGN3 receptor and signaling activation in Casparian strip formation. 22.06.2019 Beijing, China Okuda Satohiro;
11th Tri-National Arabidopsis Meeting Talk given at a conference Mechanistic insight into CIF peptide ligands binding on SGN3 receptor in Casparian strip formation 10.04.2019 Zuerich, Switzerland Okuda Satohiro;
ERA CAPS Symposium, Zurich, CH Talk given at a conference Mechanistic insights into ligand recognition, receptor activation and regulation of plant receptor kinases 31.10.2018 Zuerich, Switzerland Hothorn Michael;
91st Annual Meeting of The Japanese Biochemical Society Talk given at a conference Molecular mechanism of plant root diffusion barrier control 24.09.2018 Kyoto, Japan Savarin Julie; Okuda Satohiro;
82nd Annual Meeting of The Botanical Society of Japan Talk given at a conference Molecular mechanism of root diffusion barrier control by SGN3 - CIF peptides interaction 16.09.2018 Hiroshima, Japan Okuda Satohiro;
6th European Workshop on Plant Peptides and Receptors Talk given at a conference Molecular mechanism of plant root diffusion barrier control 06.09.2018 Malaga, Spain Okuda Satohiro;
International Brassinosteroid Signaling Conference Talk given at a conference Mechanistic insights into the activation and regulation of brassinosteroid signaling complexes 02.08.2018 San Diego, United States of America Hothorn Michael;
18th HFSP awardees meeting Poster Molecular mechanism for plant developmental control by divergent plant membrane receptors 10.07.2018 Toronto, Canada Okuda Satohiro;
Swiss Plant Science Meeting Meiringen Talk given at a conference Mechanistic insights into the activation and regulation of plant membrane receptor kinases 01.02.2018 Meiringen, Switzerland Hothorn Michael;
Department Seminar UNINE Individual talk Mechanism of plant membrane signal transduction by leucine-rich repeat receptor kinases 24.11.2017 Neuchatel, Switzerland Hothorn Michael;
Institute Seminar ZMBP University of Tuebingen Individual talk Plant signal transduction, from atoms to phenotypes and back. 14.11.2017 Tuebingen, Germany Hothorn Michael;


Associated projects

Number Title Start Funding scheme
170925 Discovery and mechanistic dissection of novel signaling pathways controlling phosphate homeostasis in eukaryotes 01.01.2017 Sinergia
205201 Structural and functional investigation of plant membrane receptor kinases and their cytoplasmic signaling cascades 01.10.2021 Project funding

Abstract

Plant as multicellular organisms require to tightly control the division, expansion and differentiation of cells within tissues and organs in order to orchestrate their growth and development. Just like animals, plants have evolved diffusible small molecule, peptide and protein ligands to achieve this goal. These molecules are sensed by plant-unique membrane receptor kinases (RKs). Plant RKs contain different extracellular ligand-binding domains, a single membrane-spanning helix and a cytoplasmic kinase domain. We have previously shown how plant RKs with leucine-rich repeat (LRR) ectodomains can specifically sense steroid and peptide ligands and we have demonstrated that different LRR-RKs require shape-complementary co-receptors for high-affinity ligand sensing and receptor activation. Using the plant brassinosteroid signaling pathway as a model system, we now propose to dissect in genetic and mechanistic detail (sub-project A):(1) how additional membrane proteins can modulate the signaling capacity of LRR-RK - co-receptor complexes (formation of higher-order signaling complexes).(2) how ligand induced interaction of receptor and co-receptor at the cell surface leads to activation of the cytoplasmic kinase domain of the receptor and to phosphorylation of down-stream components (early cytoplasmic signaling events).(3) how activation of the cytoplasmic kinase domain leads to the activation of gene expression (mechanism of the cytoplasmic signaling cascade).In addition, we want to understand the functions of plant RKs with different ectodomains (non-LRR) in plant growth and development. Specifically, we propose (sub-project B):(1) to uncover the molecular architecture of CRINKLY-type RKs (CRINKLY structure).(2) to identify and characterize CRINKLY-family ligands and define the receptor activation mechanism (CRINKLY ligands & activation).(3) to define down-stream signaling components for CRINKLY-receptors (CRINKLY cytoplasmic signaling).To achieve these goals, we will combine quantitative biochemistry and X-ray protein crystallography in vitro, with plant biochemistry and genetics in vivo. This will allow us to dissect from the physiological all the way to the mechanistic level, how different membrane signaling proteins with key roles in growth and development translate the binding of specific ligands at the cell surface into gene expression changes in the nucleus. Our work may uncover novel signaling paradigms in plants and may suggest new ways to modulate plant growth and development in the lab, and perhaps, in the field.
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