Lipid droplets; Fibrillin; Photosynthesis; Plastoglobules; Chloroplast; Phosphoprotein network; Lipid metabolism; Phosphoproteomics; Phosphorylation; Photosynthetic electron transport; Plastoquinone; Kinase
Shanmugabalaji Venkatasalam, Zita Wayne, Collombat Joy, Kessler Felix (2022), Plastoglobules: A hub of lipid metabolism in the chloroplast, in Eric Maréchal, Rébeillé Fabrice (ed.), Elsevier, London, U.K, 91-119.
Pipitone Rosa, Eicke Simona, Pfister Barbara, Glauser Gaetan, Falconet Denis, Uwizeye Clarisse, Pralon Thibaut, Zeeman Samuel C, Kessler Felix, Demarsy Emilie (2021), A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis, in
eLife, 10(e62709), 1-32.
Accossato Sonia, Kessler Felix, Shanmugabalaji Venkatasalam (2020), SUMOylation contributes to proteostasis of the chloroplast protein import receptor TOC159 during early development, in
eLife, 9(e60968), 1-16.
Pralon Thibaut, Collombat Joy, Pipitone Rosa, Ksas Brigitte, Shanmugabalaji Venkatasalam, Havaux Michel, Finazzi Giovanni, Longoni Paolo, Kessler Felix (2020), Mutation of the Atypical Kinase ABC1K3 Partially Rescues the PROTON GRADIENT REGULATION 6 Phenotype in Arabidopsis thaliana, in
Frontiers in Plant Science, 11(337), 1-18.
Shanmugabalaji Venkatasalam, Grimm Bernhard, Kessler Felix (2020), Characterization of a Plastoglobule-Localized SOUL4 Heme-Binding Protein in Arabidopsis thaliana, in
Frontiers in Plant Science, 11(2), 1-9.
Pralon Thibaut, Shanmugabalaji Venkatasalam, Longoni Paolo, Glauser Gaetan, Ksas Brigitte, Collombat Joy, Desmeules Saskia, Havaux Michel, Finazzi Giovanni, Kessler Felix (2019), Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency, in
Communications Biology, 2(1), 220-220.
Kessler Felix, Longoni Paolo (2019), How chloroplasts protect themselves from unfolded proteins, in
eLife, 8(e51430), 1-3.
Shanmugabalaji Venkatasalam, Kessler Felix (2019), CHLORAD: Eradicating Translocon Components from the Outer Membrane of the Chloroplast, in
Molecular Plant, 12(4), 467-469.
Shanmugabalaji Venkatasalam, Douet Véronique, Agne Birgit, Kessler Felix (2018), Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag, in
Journal of Visualized Experiments, 141(e58532), 1-6.
Schreier Tina B., Cléry Antoine, Schläfli Michael, Galbier Florian, Stadler Martha, Demarsy Emilie, Albertini Daniele, Maier Benjamin A., Kessler Felix, Hörtensteiner Stefan, Zeeman Samuel C., Kötting Oliver (2018), Plastidial NAD-Dependent Malate Dehydrogenase: A Moonlighting Protein Involved in Early Chloroplast Development through Its Interaction with an FtsH12-FtsHi Protease Complex, in
The Plant Cell, 30(8), 1745-1769.
Chahtane Hicham, Nogueira Füller Thanise, Allard Pierre-Marie, Marcourt Laurence, Ferreira Queiroz Emerson, Shanmugabalaji Venkatasalam, Falquet Jacques, Wolfender Jean-Luc, Lopez-Molina Luis (2018), The plant pathogen Pseudomonas aeruginosa triggers a DELLA-dependent seed germination arrest in Arabidopsis, in
eLife, 7(e37082), 1-34.
Shanmugabalaji Venkatasalam, Chahtane Hicham, Accossato Sonia, Rahire Michèle, Gouzerh Guillaume, Lopez-Molina Luis, Kessler Felix (2018), Chloroplast Biogenesis Controlled by DELLA-TOC159 Interaction in Early Plant Development, in
Current Biology, 28(16), 2616-2623.e5.
Members of the atypical ABC1-like kinase family constitute the largest group of kinases in the chloroplast. The family members ABC1K1 and -K3 play essential roles in proton gradient regulation (PGR) in photosynthesis and tocopherol biosynthesis presumably by phosphorylating tocopherol cyclase (VTE1). Moreover, ABC1K1 and -K3 physically interact but have genetically opposed functions. However, their functional mechanisms in photosynthesis are largely mysterious. Surprisingly, they near-exclusively associate with plastoglobules (PGs), a lipid subcompartment that is physically attached to the photosynthetic thylakoids by a neck-like membrane structure. Key metabolites, such as the electron carrier plastoquinone and the antioxidant tocopherol, partition between PG and thylakoids and ample evidence suggests their trafficking between the two compartments. Yet, it is not known how trafficking works. In addition to the ABC1-like kinases, PGs are decorated with metabolic enzymes (e.g. VTE1) and members of the fibrillin (FBN) family. In an apple fbn4 mutant, PGs are devoid of plastoquinone while total plastoquinone concentration is not affected. It has been suggested that FBNs play a role in trafficking and distribution between the two compartments. Several lines of evidence suggest that also in the abc1k1 mutant plastoquinone distribution and/or availability in the thylakoids may be perturbed while total plastoquinone concentration again is unchanged. In fact, abc1k1 exhibits faster saturation of photosynthetic electron transport (PET), reduced non-photochemical quenching and stronger photoinhibition under high light. These observations are consistent with plastoquinone limitation in the PET chain. However, other explanations cannot be excluded. We entertain several, mutually non-exclusive scenarios implicating the ABC1K1/-K3 kinase pair as well as FBNs that may explain these observations.1. ABC1K1/-K3, possibly together with FBNs, act indirectly on photosynthesis by regulating plastoquinone distribution or allocation to the PET chain. 2. ABC1K1/-K3 act directly by phosphorylating targets in the photosynthetic machinery or elsewhere e.g. VTE1 in PG.3. ABC1K1 and -K3 act directly by binding to components of the photosynthetic machinery or interaction partners elsewhere e.g. to each other in PG.