macromolecular assemblies; automation; structural biology; cryo electron microscope; molecular mechanism; direct electron detector; high resolution
Li Lingyun, Krasnykov Kyrylo, Homolka David, Gos Pascal, Mendel Mateusz, Fish Richard J., Pandey Radha Raman, Pillai Ramesh S. (2022), The XRN1-regulated RNA helicase activity of YTHDC2 ensures mouse fertility independently of m6A recognition, in Molecular Cell
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Yu Jun, Raia Pierre, Ghent Chloe M., Raisch Tobias, Sadian Yashar, Cavadini Simone, Sabale Pramod M., Barford David, Raunser Stefan, Morgan David O., Boland Andreas (2021), Structural basis of human separase regulation by securin and CDK1–cyclin B1, in Nature
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Hagemann H. Hans-Rudolph, Afshani Jafar (2021), Synthesis, luminescence and persistent luminescence of europium-doped strontium aluminates, Elsevier, Elsevier , 163-225.
Moser von Filseck Joachim, Barberi Luca, Talledge Nathaniel, Johnson Isabel E., Frost Adam, Lenz Martin, Roux Aurélien (2020), Anisotropic ESCRT-III architecture governs helical membrane tube formation, in Nature Communications
, 11(1), 1516-1516.
Klena Nikolai, Le Guennec Maeva, Tassin Anne‐Marie, van den Hoek Hugo, Erdmann Philipp S, Schaffer Miroslava, Geimer Stefan, Aeschlimann Gabriel, Kovacik Lubomir, Sadian Yashar, Goldie Kenneth N, Stahlberg Henning, Engel Benjamin D, Hamel Virginie, Guichard Paul (2020), Architecture of the centriole cartwheel‐containing region revealed by cryo‐electron tomography, in The EMBO Journal
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Le Guennec Maeva, Klena Nikolai, Gambarotto Davide, Laporte Marine H., Tassin Anne-Marie, van den Hoek Hugo, Erdmann Philipp S., Schaffer Miroslava, Kovacik Lubomir, Borgers Susanne, Goldie Kenneth N., Stahlberg Henning, Bornens Michel, Azimzadeh Juliette, Engel Benjamin D., Hamel Virginie, Guichard Paul (2020), A helical inner scaffold provides a structural basis for centriole cohesion, in Science Advances
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Karuppasamy Manikandan, Kusmider Beata, Oliveira Taiana M., Gaubitz Christl, Prouteau Manoel, Loewith Robbie, Schaffitzel Christiane (2017), Cryo-EM structure of Saccharomyces cerevisiae target of rapamycin complex 2, in Nature Communications
, 8(1), 1729-1729.
Prouteau Manoël, Desfosses Ambroise, Sieben Christian, Bourgoint Clélia, Lydia Mozaffari Nour, Demurtas Davide, Mitra Alok K., Guichard Paul, Manley Suliana, Loewith Robbie (2017), TORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity, in Nature
, 550(7675), 265-269.
Recent technological advances have firmly established electron microscopes as powerful tools for visualizing complex biological macromolecules at resolutions that allow atomistic interpretation. Due to the development of cryo technologies, automation and a new generation of detectors, transmission electron microscopy (TEM) is now rivalling the established technologies of X-ray crystallography and NMR for structure determination at high resolution. Cryo-EM is particularly powerful for the analysis of large macromolecular complexes, which are of fundamental importance in biological processes and a topic of intense research efforts.With this proposal we are asking for support towards the acquisition of a state-of-the-art Talos Arctica cryo-TEM system for 3D imaging of macromolecular and sub-cellular structures. High-resolution structural biology is an important pillar of modern biology, and Geneva is home to a rapidly growing community of structural biologists with proven track records and cutting-edge projects that demand the use of 3D cryo-EM technology. With the acquisition of the Talos Arctica we want to provide independence to the local community and ensure that Geneva stays competitive in this rapidly evolving field. 3D cryo-EM will integrate into the existing EM infrastructure of the Bioimaging Center at the Faculty of Sciences of the University of Geneva that will provide maintenance and support for structure determination by cryo-EM to the local and Swiss research community.