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Determining the efficacy of (waste)water ozonation for virus control: from batch experiments to pilot-scale validation

English title Determining the efficacy of (waste)water ozonation for virus control: from batch experiments to pilot-scale validation
Applicant Kohn Tamar
Number 169615
Funding scheme Project funding
Research institution Laboratoire de chimie environnementale EPFL - ENAC - IIE - LCE
Institution of higher education EPF Lausanne - EPFL
Main discipline Other disciplines of Engineering Sciences
Start/End 01.11.2016 - 31.10.2018
Approved amount 297'992.00
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All Disciplines (2)

Discipline
Other disciplines of Engineering Sciences
Other disciplines of Environmental Sciences

Keywords (6)

micropollutant; ozone; electron donating capacity; virus; water and wastewater treatment; inactivation mechanism

Lay Summary (German)

Lead
Dies Ozonung ist ein Verfahren zur Wasserbehandlung, das zunehmend an Bedeutung gewinnt. In der Schweiz werden gegenwärtig 100 Abwasserreinigungsanlagen (ARA) mit weitergehenden Verwfahren ausgerüstet, welche oft eine Ozounung beinhalten. Ozon ist ein effizientes Desinfektionsmittel, das selbst gegen sehr hartnäckige Pathogene (z.B. Kryptosporidien) wirkt. Allerdings ist die Wirksamkeit von Ozon gegen Humanviren nur schlecht untersucht. Humanviren sind häufige Auslöser von Krankheiten, die durch Wasser übertragen werden. Da Desinfektion von Trink- und Abwasser ist ein wichtiger Schritt in der Begrenzung von viralen Krankheiten, darum ist es wichtig, eine gute Wissensgrundlage zur Effizient von Ozon gegen Viren zu erarbeiten.
Lay summary

Inhalt und Ziel des Projekts

Ziel dieses Projekts ist es, die Effizient von Ozon gegen häufige Viren in Wasser und Abwasser zu bestimmen. Zu diesem Zweck haben wir ein experimentelles System entwickelt, mit dem Abbauraten von Viren im Labor gemessen werden können. Im Detail werden wir 1) eine Datanbank mit den Abbauraten von 10 wichtigen Humanviren und Indikatoren (Bateriophagen) erstellen; 2) mittels den im Labor bestimmten Abbauraten die Inaktivierung von Viren in einer Pilotanlage modellieren und dann experimentell teseten; und 3) die Mechansimen der Vireninaktivirung durch Ozon untersuchen.

 Wissenschaftlicher und gesellschaftlicher Kontext

Als Resultat diese Projekts wird die bisher umfassenste und akkurateste Datenbank zum Virenabbau durch Ozon enstehen. Diese Daten werden von verschiedenen Akteuren genutzt werden, wie z.B. von Umweltingenieuren zur Optimisierung der Wasserbehandlung, oder von Umweltbehörden zur Festsetzung von Behandlungszielen.

 

Direct link to Lay Summary Last update: 26.09.2016

Responsible applicant and co-applicants

Employees

Publications

Publication
Relationship Between Inactivation and Genome Damage of Human Enteroviruses Upon Treatment by UV254, Free Chlorine, and Ozone
Young Suzanne, Torrey Jason, Bachmann Virginie, Kohn Tamar (2020), Relationship Between Inactivation and Genome Damage of Human Enteroviruses Upon Treatment by UV254, Free Chlorine, and Ozone, in Food and Environmental Virology, 12(1), 20-27.
Proxies to monitor the inactivation of viruses by ozone in surface water and wastewater effluent
Wolf Camille, Pavese Annalisa, von Gunten Urs, Kohn Tamar (2019), Proxies to monitor the inactivation of viruses by ozone in surface water and wastewater effluent, in Water Research, 166, 115088-115088.
Differences in Viral Disinfection Mechanisms as Revealed by Quantitative Transfection of Echovirus 11 Genomes
Torrey Jason, von Gunten Urs, Kohn Tamar (2019), Differences in Viral Disinfection Mechanisms as Revealed by Quantitative Transfection of Echovirus 11 Genomes, in Applied and Environmental Microbiology, 85(14), e00961-19.
Kinetics of Inactivation of Waterborne Enteric Viruses by Ozone
Wolf Camille, von Gunten Urs, Kohn Tamar (2018), Kinetics of Inactivation of Waterborne Enteric Viruses by Ozone, in Environmental Science & Technology, 52(4), 2170-2177.

Collaboration

Group / person Country
Types of collaboration
Prof. Krista Wigginton, University of Michigan United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
Zurich Water Works Switzerland (Europe)
- Research Infrastructure

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
ISFEV 2018 Talk given at a conference Virus Inactivation by Ozone in Natural Matrices: Potential Proxies for Viral Inactivation 07.10.2018 Tempe, AZ, United States of America von Gunten Urs; Wolf Camille; Kohn Tamar;
ISFEV 2018 Talk given at a conference DETERMINING VIRAL GENOME FUNCTIONALITY LOSS AFTER VARIOUS DISINFECTION TREATMENTS USING A NOVEL TRANSFECTION-BASED MOST PROBABLE NUMBER ASSAY 07.10.2018 Tempe, AZ, United States of America von Gunten Urs; Kohn Tamar; Torrey Jason;
International Ozone Association: Ozone and Advanced Oxidation Solutions for Emerging Pollutants of Concern to the Water and the Environment Talk given at a conference VIRUS INACTIVATION BY OZONE NATURAL MATRICES: POTENTIAL PROXIES FOR VIRAL INACTIVATION 05.09.2018 Lausanne, Switzerland von Gunten Urs; Kohn Tamar; Wolf Camille;
International Ozone Association: Ozone and Advanced Oxidation Solutions for Emerging Pollutants of Concern to the Water and the Environment Talk given at a conference TITLE: DETERMINING VIRAL INACTIVATION AFTER OZONE TREATMENT USING A NOVEL TRANSFECTION-BASED MOST PROBABLE NUMBER ASSAY 05.09.2018 Lausanne, Switzerland Kohn Tamar; Torrey Jason; von Gunten Urs;
IWA International Symposium on Health-Related Water Microbiology Poster Virus inactivation by ozone: Kinetics and influence of water quality parameters 15.05.2017 Chapel Hill, NC, United States of America Kohn Tamar; Wolf Camille; von Gunten Urs;


Awards

Title Year
Gordon Research conference Enironmental Science: Water. Best poster - runner up 2016

Associated projects

Number Title Start Funding scheme
163270 Association between environmental persistence and disinfection resistance in waterborne enteroviruses 01.11.2015 Project funding
181975 Reactions of ozone with water matrix components: reactive sites and oxidation by-product formation 01.11.2018 Project funding

Abstract

This project seeks to evaluate the efficacy of water and wastewater ozonation with respect to the inactivation of waterborne viruses. Ozone has been utilized in water treatment for several decades, both as a disinfectant and for the control of taste and odor compounds. Increasingly, ozone is also been applied for water reuse and wastewater treatment purposes. In Switzerland, for example, new legislation requires that the 100 largest wastewater treatment plants introduce an advanced treatment step, such as ozonation. While the main purpose of the advanced treatment is the removal of micropollutants, ozonation of the wastewater will also improve its microbial quality. The appeal of ozone as a disinfectant lies in its potency toward pathogens (e.g., Cryptosporidium) that are resistant to more traditional disinfectants, such as free chlorine. The effect of ozone on waterborne viruses, however, is only poorly understood. This is an important knowledge gap, as viruses contribute significantly to waterborne disease outbreaks. Because disinfection is the most important barrier to virus dissemination, the efficacy of disinfectants such as ozone toward viruses must be known. However, the currently available literature data on virus inactivation by ozone is inconsistent, and reported inactivation rate constants for the same viruses vary by up to five orders of magnitude. As such, it is currently not possible to establish appropriate treatment practices for virus control. For this project, we have developed an experimental system which allows us to reproducibly determine virus inactivation kinetics by ozone under a variety of environmental conditions. Using this approach, we will first establish a high-quality data set of kinetic inactivation parameters for ten human and bacterial viruses. Second, we will use these data to estimate virus inactivation in a pilot-scale treatment system, and validate our predictions experimentally. This is an important step that will allow us to transfer the kinetic data obtained in batch to systems representative of actual treatment applications. And finally, we will characterize the main mechanisms involved in the ozone-induced inactivation of viruses, to gain fundamental insight into the mode of action underlying the efficacy of ozone. Overall, this work will allow us to understand and optimize ozonation for virus control, and it will provide useful tools to estimate virus removal during water and wastewater treatment. This will, for example, enable us to assess the effect of wastewater ozonation on the microbial quality of the receiving waters, or to evaluate the role of ozone in meeting treatment standards for water reuse. The outcomes of this project will thus be relevant to federal and cantonal authorities, regulatory agencies, treatment engineers, and environmental scientists.
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