Pseudomonas putida; extracellular polysaccharide; Quorum sensing; biofilm matrix; Burkholderia cenocepacia
Higgins Steven, Gualdi Stefano, Pinto‐Carbó Marta, Eberl Leo (2020), Copper resistance genes of Burkholderia cenocepacia H111 identified by transposon sequencing, in Environmental Microbiology Reports
, 12(2), 241-249.
Sathe Santosh, Mathew Anugraha, Agnoli Kirsty, Eberl Leo, Kümmerli Rolf (2019), Genetic architecture constrains exploitation of siderophore cooperation in the bacterium Burkholderia cenocepacia, in Evolution Letters
, 3(6), 610-622.
Richter Anja M., Fazli Mustafa, Schmid Nadine, Shilling Rebecca, Suppiger Angela, Givskov Michael, Eberl Leo, Tolker-Nielsen Tim (2019), Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia, in Frontiers in Microbiology
, 9, 3286.
Spiewak Helena L, Shastri Sravanthi, Zhang Lili, Schwager Stephan, Eberl Leo, Vergunst Annette C, Thomas Mark S (2019), Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition., in MicrobiologyOpen
Toyofuku Masanori, Nomura Nobuhiko, Eberl Leo (2019), Types and origins of bacterial membrane vesicles, in Nature Reviews Microbiology
, 17(1), 13-24.
Jenul Christian, Sieber Simon, Daeppen Christophe, Mathew Anugraha, Lardi Martina, Pessi Gabriella, Hoepfner Dominic, Neuburger Markus, Linden Anthony, Gademann Karl, Eberl Leo (2018), Biosynthesis of fragin is controlled by a novel quorum sensing signal, in Nature Communications
, 9(1), 1297-1297.
Gomes Margarida C., Tasrini Yara, Subramoni Sujatha, Agnoli Kirsty, Feliciano Joana R., Eberl Leo, Sokol Pamela, O’Callaghan David, Vergunst Annette C. (2018), The afc antifungal activity cluster, which is under tight regulatory control of ShvR, is essential for transition from intracellular persistence of Burkholderia cenocepacia to acute pro-inflammatory infection, in PLOS Pathogens
, 14(12), e1007473-e1007473.
Fazli Mustafa, Rybtke Morten, Steiner Elisabeth, Weidel Elisabeth, Berthelsen Jens, Groizeleau Julie, Bin Wu, Zhi Boo Zhao, Yaming Zhang, Kaever Volkhard, Givskov Michael, Hartmann Rolf W., Eberl Leo, Tolker-Nielsen Tim (2017), Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB, in MicrobiologyOpen
, 6(4), e00480-e00480.
Agnoli Kirsty, Freitag Roman, Gomes Margarida C., Jenul Christian, Suppiger Angela, Mannweiler Olga, Frauenknecht Carmen, Janser Daniel, Vergunst Annette C., Eberl Leo (2017), Use of Synthetic Hybrid Strains To Determine the Role of Replicon 3 in Virulence of the Burkholderia cepacia Complex, in Applied and Environmental Microbiology
, 83(13), e00461-17.
Liu Yilei, Lardi Martina, Pedrioli Alessandro, Eberl Leo, Pessi Gabriella (2017), NtrC-dependent control of exopolysaccharide synthesis and motility in Burkholderia cenocepacia H111, in PLOS ONE
, 12(6), e0180362-e0180362.
Toyofuku Masanori, Morinaga Kana, Hashimoto Yohei, Uhl Jenny, Shimamura Hiroko, Inaba Hideki, Schmitt-Kopplin Philippe, Eberl Leo, Nomura Nobuhiko (2017), Membrane vesicle-mediated bacterial communication, in The ISME Journal
, 11(6), 1504-1509.
Schmid Nadine, Suppiger Angela, Steiner Elisabeth, Pessi Gabriella, Kaever Volkhard, Fazli Mustafa, Tolker-Nielsen Tim, Jenal Urs, Eberl Leo (2017), High intracellular c-di-GMP levels antagonize quorum sensing and virulence gene expression in Burkholderia cenocepacia H111, in Microbiology
, 163(5), 754-764.
In nature bacteria exist predominantly as surface-associated multispecies biofilms. While most biofilms are beneficial or even essential they can also cause problems in industrial settings or in the clinic, as persistent and chronic infections are often intrinsically linked with the formation of biofilms. Moreover, bacteria living in biofilms are much more resistant to antibiotics than planktonic cells. Biofilm cells are completely embedded in a complex matrix composed of polysaccharides, proteins, and nucleic acids, which not only keeps the cells in close contact to each other but also is diffusion barrier. Hence, biofilms represent ideal environments for small molecule mediated cell-to-cell communication, referred to as quorum sensing (QS). While work of the past years has demonstrated that QS plays an important role in biofilm formation in many bacteria, knowledge on the underlying molecular mechanisms is scarce. The present proposal aims at elucidating the importance of QS in biofilm formation in two model organisms, Burkholderia cenocepacia H111 and Pseudomonas putida IsoF. We will use B. cenocepacia H111 as a model to visualize the temporal and spatial expression patterns of different biofilm matrix components, including two polysaccharides, the large surface protein BapA and the BclACB lectins. We are particularly interested to unravel the role of the RpfFR QS system, which relies on the fatty acid signal cis-2-dodecenoic acid (BDSF), and of the downstream secondary messenger c-di-GMP in the expression of matrix components. We will use the other model organism, P. putida IsoF, to uncover the QS-dependent production of the biosurfactant putisolvin and to analyse how this molecule affects biofilm formation. We will also use this strain to investigate fundamental questions of bacterial cell-to-cell communication, namely under which conditions QS is truly used for neighbor communication to regulate expression of common goods as the QS paradigm would suggest, and when QS is only used for self-communication to control the expression of asocial traits.The proposed project is expected to significantly increase our knowledge of the mechanisms and signal transduction pathways that underlie biofilm formation. This may allow to develop novel strategies for the avoidance, eradication and manipulation of biofilms.