insect; egg elicitors; innate immunity; pathway crosstalk; oviposition; defense gene expression; Arabidopsis thaliana
Groux Raphaël, Stahl Elia, Gouhier-Darimont Caroline, Kerdaffrec Envel, Jimenez-Sandoval Pedro, Santiago Julia, Reymond Philippe (2021), Arabidopsis natural variation in insect egg-induced cell death reveals a role for LECTIN RECEPTOR KINASE-I.1, in Plant Physiology
, 185, 440-455.
Stahl Elia, Brillatz Théo, Ferreira Queiroz Emerson, Marcourt Laurence, Schmiesing André, Hilfiker Olivier, Riezman Isabelle, Riezman Howard, Wolfender Jean-Luc, Reymond Philippe (2020), Phosphatidylcholines from Pieris brassicae eggs activate an immune response in Arabidopsis, in eLife
, 9, e60293.
Orlovskis Zigmunds, Reymond Philippe (2020), Pieris brassicae eggs trigger inter‐plant systemic acquired resistance against a foliar pathogen in Arabidopsis, in New Phytologist
, 228, 1652-1661.
Marquis Valentin, Smirnova Ekaterina, Poirier Laure, Zumsteg Julie, Schweizer Fabian, Reymond Philippe, Heitz Thierry (2020), Stress‐ and pathway‐specific impacts of impaired jasmonoyl‐isoleucine (JA‐Ile) catabolism on defense signaling and biotic stress resistance, in Plant, Cell & Environment
, 43, 1578-1570.
Peñuelas María, Monte Isabel, Schweizer Fabian, Vallat Armelle, Reymond Philippe, García-Casado Gloria, Franco-Zorrilla Jose M., Solano Roberto (2019), Jasmonate-Related MYC Transcription Factors Are Functionally Conserved in Marchantia polymorpha, in The Plant Cell
, 31(10), 2491-2509.
Gouhier-Darimont Caroline, Stahl Elia, Glauser Gaetan, Reymond Philippe (2019), The Arabidopsis Lectin Receptor Kinase LecRK-I.8 Is Involved in Insect Egg Perception, in Frontiers in Plant Science
, 10(623), 1-10.
Monte Isabel, Ishida Sakiko, Zamarreño Angel M., Hamberg Mats, Franco-Zorrilla José M., García-Casado Gloria, Gouhier-Darimont Caroline, Reymond Philippe, Takahashi Kosaku, García-Mina José M., Nishihama Ryuichi, Kohchi Takayuki, Solano Roberto (2018), Ligand-receptor co-evolution shaped the jasmonate pathway in land plants, in Nature Chemical Biology
, 14(5), 480-488.
Blanc Catherine, Coluccia Fania, L’Haridon Floriane, Torres Martha, Ortiz-Berrocal Marlene, Stahl Elia, Reymond Philippe, Schreiber Lukas, Nawrath Christiane, Métraux Jean-Pierre, Serrano Mario (2018), The Cuticle Mutant eca2 Modifies Plant Defense Responses to Biotrophic and Necrotrophic Pathogens and Herbivory Insects, in Molecular Plant-Microbe Interactions
, 31(3), 344-355.
Stahl Elia, Hilfiker Olivier, Reymond Philippe (2018), Plant-arthropod interactions: who is the winner?, in The Plant Journal
, 93(4), 703-728.
Chini Andrea, Monte Isabel, Zamarreño Angel M, Hamberg Mats, Lassueur Steve, Reymond Philippe, Weiss Sally, Stintzi Annick, Schaller Andreas, Porzel Andrea, García-Mina José M, Solano Roberto (2018), An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis, in Nature Chemical Biology
, 14(2), 171-178.
Schweizer Fabian, Heidel-Fischer Hanna, Vogel Heiko, Reymond Philippe (2017), Arabidopsis glucosinolates trigger a contrasting transcriptomic response in a generalist and a specialist herbivore, in Insect Biochemistry and Molecular Biology
, 85, 21-31.
Plants are challenged by numerous herbivorous insects and have developed sophisticated strategies to fend off these attackers. In turn, insects have evolved mechanisms to suppress plant defenses or to hijack signaling pathways for their own advantage. Eggs deposited on plants by insects represent a threat as they develop into feeding larvae. Earlier studies established that plants detect egg-associated elicitors and activate direct and indirect defenses. Recent years have seen progress in the molecular understanding of egg-induced responses in Arabidopsis thaliana. Genetic and biochemical analyses have revealed that egg perception triggers an innate immunity response that is regulated by the salicylic acid (SA) pathway. This finding was unexpected since the SA pathway is normally engaged to fight fungal and bacterial pathogens, whereas plants resist insect attack by activating jasmonic acid (JA)-dependent defenses. However, egg-induced SA accumulation was shown to protect plants against infection by the biotroph pathogen Pseudomonas syringae by activating a systemic acquired resistance (SAR). This SAR benefited the development of young hatching larvae and suggests that this phenomenon may have evolved as a strategy to prevent the detrimental effect of bacterial pathogens on feeding larvae. In addition, egg-induced SA accumulation suppressed JA-dependent gene expression by targeting key MYC transcription factors that are crucial for defense against feeding larvae. Again, this SA/JA cross talk enhanced larval performance. These intriguing findings constitute two independent examples of manipulation of a plant signaling pathway by the attacker. A major challenge is to identify egg-associated elicitors and plant receptors that lead to activation of the SA pathway. It is also necessary to better understand the biological relevance of egg-induced SAR and the precise regulation of SA/JA cross talk.The proposed research project has three objectives. The first objective is to identify a novel egg elicitor and how it is perceived by Arabidopsis. Preliminary work has identified a candidate molecule and a potential receptor that would represent the first known elicitor/receptor pair from a plant-insect interaction and would open the way for interesting structure/function analyses. The second objective is to characterize the importance of egg-induced SAR and its role against a range of biotic factors that interact with Arabidopsis. Results from this part may lead to interesting applied aspects. The third objective is to investigate how egg-induced SA/JA cross talk is modulated and the role of independent regulation steps. We also hope to identify new genes that participate in this process.This project aims at a better knowledge of plant-insect interactions. One emerging theme is that insect eggs have evolved distinct ways to highjack the SA pathway for the benefit of larvae. We hope to unveil interesting mechanisms that may apply to diverse interactions between plants and biotic factors.