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Analysis of LRX proteins as potential regulators of Ca2+ fluxes

English title Analysis of LRX proteins as potential regulators of Ca2+ fluxes
Applicant Ringli Christoph
Number 166577
Funding scheme Project funding (Div. I-III)
Research institution Institut für Pflanzen- und Mikrobiologie Universität Zürich
Institution of higher education University of Zurich - ZH
Main discipline Botany
Start/End 01.04.2016 - 30.04.2019
Approved amount 343'640.00
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All Disciplines (2)

Discipline
Botany
Cellular Biology, Cytology

Keywords (6)

LRR-extensins; LRX proteins; Arabidopsis thaliana; cell wall; development; Ca2+ and ROS

Lay Summary (German)

Lead
Ein spezifisches Charakteristikum von Pflanzen ist die Zellwand, welche jede einzelne Zelle umgibt. Diese schützt die Zelle, gibt ihr eine definierte Form und ist eine wichtige regulatorische Komponente im Zellwachstum. Letzteres deshalb, weil Pflanzenzellen nur wachsen können, wenn die Zellwand sich ausdehnt. Um dies zu ermöglichen, muss ein Informationsaustausch zwischen dem Zellinnern und der Zellwand bestehen.
Lay summary

Inhalt des Forschungsprojektes

LRR-Extensine sind in der Zellwand lokalisierte Proteine, welche in den Zellwandaufbau involviert sind. Dieses Projekt untersucht die Funktion(en) dieser Proteine im Detail. Indizien sprechen für eine Funktion an der äusseren Schicht der Zelle, der Plasmamembran, wo die LRX Proteine möglicherweise die Aktivität von Proteinen beeinflussen. Da LRX Proteine mit der Zellwand verbunden sind, könnten sie die Funktion haben, die Plasmamembran mit der Zellwand zu verbinden. Diese Hypothese soll in diesem Projekt untersucht werden. Zudem ist es das Ziel, die Art der Interaktion zwischen LRX Proteinen und der Plasmamembran zu charakterisieren, und die biologische Relevanz dieser Bindung zu definieren. 

 

Wissenschaftlicher und gesellschaftlicher Kontext des Forschungsprojektes

Das Projekt befasst sich mit der molekularen Kommunikation zwischen verschiedenen Zonen von pflanzlichen Zellen. Dies ist aus dem Blickwinkel der Grundlagenforschung relevant. Allerdings ist das bessere Verständnis des Zellwandaufbaus auch deshalb interessant, weil die Zellwandmaterialien von grossem industriellen Interesse sind, z.B. in der Prouktion von Geliermitteln, Papier oder Biobrennstoffen.
Direct link to Lay Summary Last update: 06.04.2016

Responsible applicant and co-applicants

Employees

Publications

Publication
Extracellular matrix sensing by FERONIA and Leucine‐Rich Repeat Extensins controls vacuolar expansion during cellular elongation in Arabidopsis thaliana
Dünser Kai, Gupta Shibu, Herger Aline, Feraru Mugurel I, Ringli Christoph, Kleine‐Vehn Jürgen (2019), Extracellular matrix sensing by FERONIA and Leucine‐Rich Repeat Extensins controls vacuolar expansion during cellular elongation in Arabidopsis thaliana, in The EMBO Journal, 38(7), e100353-e100353.
Mutations in the Arabidopsis ROL17/isopropylmalate synthase 1 locus alter amino acid content, modify the TOR network, and suppress the root hair cell development mutant lrx1
Schaufelberger Myriam, Galbier Florian, Herger Aline, de Brito Francisco Rita, Roffler Stefan, Clement Gilles, Diet Anouck, Hörtensteiner Stefan, Wicker Thomas, Ringli Christoph (2019), Mutations in the Arabidopsis ROL17/isopropylmalate synthase 1 locus alter amino acid content, modify the TOR network, and suppress the root hair cell development mutant lrx1, in Journal of Experimental Botany, 70(8), 2313-2323.
LRX Proteins Play a Crucial Role in Pollen Grain and Pollen Tube Cell Wall Development
Fabrice Tohnyui Ndinyanka, Vogler Hannes, Draeger Christian, Munglani Gautam, Gupta Shibu, Herger Aline G., Knox Paul, Grossniklaus Ueli, Ringli Christoph (2018), LRX Proteins Play a Crucial Role in Pollen Grain and Pollen Tube Cell Wall Development, in Plant Physiology, 176(3), 1981-1992.

Collaboration

Group / person Country
Types of collaboration
Prof. Dr. Pau Knox, University of Leeds Great Britain and Northern Ireland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. U. Grossniklaus, Institute of Plant Biology, University of Zürich Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Dr. Beat Frey, WSL Birmensdorf Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Dr. Jorge Muschietti, University of Buenos Aires Argentina (South America)
- Publication
Dr. Grégory Mouille, INRA, Versailles France (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Dr. Gilles Clement, INRA, Versailles France (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Jürgen Kleine-Vehn, BOKU Vienna, Austria Austria (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication

Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions TechDays/Nights Referat; wie die Ernährung die Menschheit weitergebracht hat German-speaking Switzerland 2019

Associated projects

Number Title Start Funding scheme
138472 The TOR (Target of Rapamycin) signalling pathway - a new modulator of cell wall development 01.02.2012 Project funding (Div. I-III)
192495 The dynamics of LRX-RALF-FER module in the regulation of cell wall development 01.04.2020 Project funding (Div. I-III)
192495 The dynamics of LRX-RALF-FER module in the regulation of cell wall development 01.04.2020 Project funding (Div. I-III)

Abstract

Plant cells are surrounded by cell walls provide protection against pathogens, wounding, and abiotic stresses. Typically, they are composed of cellulose, hemicellulose, pectins, and structural proteins. Plant cell growth is driven by the internal turgor pressure of the cell but is limited by the cell wall, which has to expand for successful yielding of the cell. Expansion of the cell wall requires metabolic activity in the cell to supply new wall material and the integration of this material into the expanding cell wall. Hence, a network of proteins establishing the cytoplasm - cell wall continuum is necessary to survey, control, and regulate these complex processes in a temporally and spatially coordinated fashion.Leucine-rich repeat-extensin (LRX) proteins are extracellular proteins that are anchored to the cell wall through the extensin (hydroxyproline-rich glycoprotein) domain and bind, via their LRR domain, a so far unknown interaction partner. Of the eleven LRX proteins encoded by the genome of Arabidopsis thaliana, several have been shown to influence cell wall formation. We have mutants of different Arabidopsis LRX genes that are affected in the root, the shoot, or pollen tubes, giving a choice of cellular systems to be used for the different analyses. In addition, a number of rol (repressor of lrx1) mutants were isolated that suppress the root hair growth defect of the lrx1 mutant. Whole genome sequencing of five of these mutants revealed candidate genes for the mutated rol loci, providing an insight into genes and processes that can suppress lrx1. We recently have obtained results indicating that the LRR domain of several LRX proteins associates with the plasma membrane, while the extensin domain has been shown to interact with the cell wall. We propose to investigate this observation in detail as it provides evidence for LRX proteins being plasma membrane. In parallel, we aim to identify the interaction partner(s) of the LRR domain of LRXs, which are likely to localize in or at the plasma membrane. Finally, several of the isolated rol mutants are affected in the homeostasis of reactive oxygen species (ROS). The biological relevance of this observation will be investigated in the rol mutants, while a detailed molecular analysis of the mutated genes will be limited to one or two of these lines.The proposed project will determine the contribution of LRX proteins to the regulation of Ca2+- and possibly ROS- related processes. If LRX proteins indeed represent cell-wall attached linker proteins, this should be substantiated by the planned experiments. Cytological experiments and genetic analyses should also shed light on the potential function of LRXs and their possible interaction partner(s). Together, these experiments should provide a better insight into the poorly understood function of LRX proteins.
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