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DESIGN OF NANOREACTORS AND ARTIFICIAL ORGANELLES BASED ON POLYMER SUPRAMOLECULAR ASSEMBLIES

English title DESIGN OF NANOREACTORS AND ARTIFICIAL ORGANELLES BASED ON POLYMER SUPRAMOLECULAR ASSEMBLIES
Applicant Palivan Cornelia
Number 165859
Funding scheme Project funding
Research institution Physikalische Chemie Departement Chemie Universität Basel
Institution of higher education University of Basel - BS
Main discipline Physical Chemistry
Start/End 01.05.2016 - 31.05.2017
Approved amount 72'046.00
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Keywords (5)

cascade reactions; amphiphilic copolymers; self-assembly; enzymes; nanoreactors

Lay Summary (French)

Lead
La nanotechnologie propose de plus en plus des solutions innovatrices pour répondre à des nécessités dans le domaine des traitements médicaux, de production des substances ou pour saisir d’une manière sensitive et rapide la présence des molécules d’intérêt. Par exemple, l’utilisation des réactions spécifiques qui ont lieu dans l’espace minuscule des compartiments avec des dimensions nanométriques, qu’on nomme des nanoréacteurs pourra servir pour produire des substances d’intérêt dans les meilleurs conditions de control et d’efficacité. Les nanoréacteurs sont générés par encapsulation des molécules actives (enzymes, protéines, vitamines, DNA) dans des compartiments avec des dimensions nanométriques qui ont les parois perméables afin de permettre un échange des molécules avec le milieu extérieur. Cet échange moléculaire permet d’ entretenir la réaction chimique dans la cavité des nanoréacteurs et donc leur fonctionnalité.
Lay summary

Contenu et objectives du travail de recherche

Le projet a comme but de développer des réactions en cascade dans différents compartiments sois par co-encapsulation des différentes protéines dans un seul compartiment, sois par encapsulation  chaque type de protéine dans  différents compartiments situés dans la proximité. Pour que les réactions aient lieux dans l’espace des compartiments, des protéines membranaires vont être insérés dans leurs parois. Ces protéines serviront comme des « portes » nanométriques pour des molécules qui font partie des réactions : les substrats nécessaires pour l’activité des enzymes et  les produits des réactions qui vont être libérés des nanoréacteurs. La comparaison de l’efficacité des réactions va servir pour obtenir les meilleures conditions pour la production des substances désirées.

Contexte scientifique et social du project de recherche

L’objectif de ces recherches sera d’étudier des réactions enzymatiques en cascade dans des compartiments avec des dimensions nanométriques afin de comprendre leurs conditions et de les améliorer pour produire et libérer d’une manière contrôlée des molécules désirées, comme par exemple des biomolécules.
Notre équipes suisse-chinoise vont unir leurs efforts de recherche pour une avancée dans le domaine de surfaces multifonctionnelles aves des propriétés bio-sensibles pour les applications médicales.

Keywords
 polymer compartments, nanoreacteurs, cascade reactions, enzymes, proteins

Direct link to Lay Summary Last update: 15.04.2016

Responsible applicant and co-applicants

Employees

Publications

Publication
Biomimetic artificial organelles with in vitro and in vivo activity triggered by reduction in microenvironment
Einfalt T., Witzigmann D., Edlinger C., Sieber S., Goers R., Najer A., Spulber M., Onaca-Fischer O., Huwyler J., Palivan C. G. (2018), Biomimetic artificial organelles with in vitro and in vivo activity triggered by reduction in microenvironment, in Nature Communications, 9(1), 1127-1127.
Antioxidant functionalized polymer capsules to prevent oxidative stress
Larrañaga Aitor, Isa Isma Liza Mohd, Patil Vaibhav, Thamboo Sagana, Lomora Mihai, Fernández-Yague Marc A., Sarasua Jose-Ramon, Palivan Cornelia G., Pandit Abhay (2018), Antioxidant functionalized polymer capsules to prevent oxidative stress, in Acta Biomaterialia, 67, 21-31.
Biomimetic Strategy To Reversibly Trigger Functionality of Catalytic Nanocompartments by the Insertion of pH-Responsive Biovalves
Edlinger Christoph, Einfalt Tomaz, Spulber Mariana, Car Anja, Meier Wolfgang, Palivan Cornelia G. (2017), Biomimetic Strategy To Reversibly Trigger Functionality of Catalytic Nanocompartments by the Insertion of pH-Responsive Biovalves, in Nano Letters, 17(9), 5790-5798.
Bio-catalytic nanocompartments for in situ production of glucose-6-phosphate
Lomora M., Gunkel-Grabole G., Mantri S., Palivan C. G. (2017), Bio-catalytic nanocompartments for in situ production of glucose-6-phosphate, in Chemical Communications, 53(73), 10148-10151.
Biopores/membrane proteins in synthetic polymer membrane
Garni M., Thamboo S., Schoenenberger C.A., Palivan C.G. (2017), Biopores/membrane proteins in synthetic polymer membrane, in BBA- Biomembranes, 1859(4), 619-638.
Angiopep2-functionalized polymersomes for targeted doxorubicin delivery to glioblastoma cells
Figueiredo Patrícia, Balasubramanian Vimalkumar, Shahbazi Mohammad-Ali, Correia Alexandra, Wu Dalin, Palivan Cornelia G., Hirvonen Jouni T., Santos Hélder A. (2016), Angiopep2-functionalized polymersomes for targeted doxorubicin delivery to glioblastoma cells, in International Journal of Pharmaceutics, 511(2), 794-803.
Artificial Organelles: Reactions inside Protein–Polymer Supramolecular Assemblies
Garni Martina, Einfalt Tomaž, Lomora Mihai, Car Anja, Meier Wolfgang, Palivan Cornelia G. (2016), Artificial Organelles: Reactions inside Protein–Polymer Supramolecular Assemblies, in CHIMIA International Journal for Chemistry, 70(6), 424-427.

Collaboration

Group / person Country
Types of collaboration
Prof.Dr. W. Meier, University of Basel Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
PD Dr. S. Gros, Universitäts-Kinderspital beider Basel (UKBB) Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Invited talk at the University of Tubingen Individual talk Protein-polymer supramolecular assemblies: Bio-nano functional systems 23.01.2017 Tubingen, Germany Palivan Cornelia;
3rd International Conference on Bioinspired and Biobased Chemistry N.I.C.E. Talk given at a conference Smart self-assembled functional nanosystems: Polymer membranes decorated with proteins 19.10.2016 Nice, France Palivan Cornelia;
3rd International Conference on Bioinspired and Biobased Chemistry N.I.C.E. Talk given at a conference 'Smart' surfaces as sensors for detection of pH changes 16.10.2016 Nice, France Palivan Cornelia; Lomora Mihai;
29th International Symposium on Pediatric Surgical Research Talk given at a conference Aqp1 influences cell water permeability and mobility 08.09.2016 Frankfurt, Germany Lomora Mihai;
3rd International Conference on Analytical Chemistry (ROICAC'2016) Talk given at a conference Polymersomes with engineered selective membrane permeability as novel biosensing nanodevices 28.08.2016 Iasi, Romania Lomora Mihai;
252nd ACS National Meeting Talk given at a conference Cascade reactions in confined spaces at the nanoscale for replacing part of an impaired metabolic pathway 24.08.2016 Philadelphia, United States of America Lomora Mihai;
252nd ACS National Meeting Talk given at a conference Biopores inside synthetic membranes of giant unilamellar vesicles (GUVs) as model of cell membranes 24.08.2016 Philadelphia, United States of America Lomora Mihai;
World Chemistry Conference Talk given at a conference “Smart” self-assembled functional nanosystems based on polymer membranes decorated with proteins 08.08.2016 Toronto, Canada Palivan Cornelia;


Associated projects

Number Title Start Funding scheme
140302 PROTEIN-POLYMER SUPRAMOLECULAR ASSEMBLIES IN THE DESIGN OF ANTIOXIDANT NANOREACTORS AND PROCESSORS 01.06.2012 Project funding
172604 BIOINSPIRED FUNCTIONAL PROTEIN-POLYMER SUPRAMOLECULAR NANOASSEMBLIES 01.07.2017 Project funding
139133 Structure, dynamics and interactions of paramagnetic centers characterised by Electrn Paramagnetic Resonance 01.12.2011 R'EQUIP

Abstract

Design of new materials and active assemblies by understanding the expression of nature’s intelligence and mimicking its structures and functions is on focus today in various domains that include chemistry, medicine, electronics, and materials science. In this respect, a key strategy is to combine biomolecules that induce functionality through their specific biologic activity with synthetic structures with a variety of architectures (micelles, vesicles, tubes, particles).In this project, we plan to develop a platform of cascade reaction compartments at nanoscale for efficient and controlled production of molecules by a combination of physical chemistry, nanoscience and enzymatic reactions. Our aim is to encapsulate/insert biomolecules (proteins, enzymes) in synthetic compartments to create nanoreactors in which a cascade reaction is performed in situ inside one compartment, or it takes place between different compartments. The necessity to develop cascade reactions in compartments with nanometer sizes originates from the increasing evidence of uncontrolled release associated with the conventional drug delivery systems, and the aim to produce in a space- and time-controlled manner active molecules necessary for several severe pathological conditions, such as cancer, glycogen storage diseases, and infectious diseases.The project will evaluate the efficacy of cascade reactions in different topologic compartments in order to understand the conditions necessary to support a successful application, as for example introduction of artificial organelles as cellular implants mimicking natural ones. Our strategy to study cascade reactions inside confined space will provide structural and functional details to choose the type of cascade reaction space in a rational design by straightforward change of the biomolecules or the overall polymer assembly. The project will create the premises to use the platform of nanoreactors for applications in various domains, such as medicine, food science, environmental sciences, and technology.
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