Cardiac ion channels; High-Throughput Purification; Automated Peptide Synthesis; Antimicrobials; Preparative HPLC; Chemical Space; Drug conjugates; Peptides
Abdel-Sayed Philippe, Kaeppeli Ariane, Siriwardena Thissa, Darbre Tamis, Perron Karl, Jafari Paris, Reymond Jean-Louis, Pioletti Dominique P., Applegate Lee Ann, Anti-Microbial Dendrimers against Multidrug-Resistant P. aeruginosa Enhance the Angiogenic Effect of Biological Burn-wound Bandages, in Scientific Reports
, 2016, 22020.
Kwok Albert, Eggimann Gabriela A., Heitz Marc, Reymond Jean-Louis, Hollfelder Florian, Darbre Tamis, Efficient Transfection of siRNA by Peptide Dendrimer–Lipid Conjugates, in ChemBioChem
, 17(23), 2223-2229.
Bergmann M, Michaud G, Visini R, Jin X, Gillon E, Stocker A, Imberty A, Darbre T, Reymond JL, Multivalency effects on Pseudomonas aeruginosa biofilm inhibition and dispersal by glycopeptide dendrimers targeting lectin LecA., in Organic & Biomolecular Chemistry
, 2016(14), 138-148.
Michaud G., Visini R., Bergmann M., Salerno G., Bosco R., Gillon E., Richichi B., Nativi C., Imberty A., Stocker A., Darbre T., Reymond JL, Overcoming antibiotic resistance in Pseudomonas aeruginosa biofilms using glycopeptide dendrimers., in Chemical Science
, 2016(7), 166-182.
Our research projects heavily rely on the high-throughput synthesis of structurally diverse peptides. These projects include the exploration of the chemical space of topologically diverse peptides, the development of transfection agents and antimicrobials, the development of Ruthenium peptide conjugates as a novel type of anti-cancer agents, and the study of the regulation of cardiac ion channels. These projects require the ability to synthesize tens to hundreds of pure peptides and peptide constructs in a matters of days or weeks. To achieve this goal, we have recently acquired an automatic microwave peptide synthesizer (CEM Liberty Blue), which makes it possible to complete a peptide synthesis automatically with 2-5 hours, which is much faster and gives significantly higher yields than with room-temperature synthesis. This has raised our requirements for peptide purification to increase throughput. The present proposal is asking for support of a new automated peptide purification infrastructure in form of a preparative HPLC with an integrated auto-sampler, and a lyophilizer to concentrate pure fractions. These elements will replace an older manually operated preparative HPLC and complement an already existing automated preparative HPLC system, to make it possible to exploit our newly acquired peptide synthesis capacity.