Kummer Eva, Ban Nenad (2018), Conformational Switching of the Nuclear Exosome during Ribosome Biogenesis
, 57(32), 4765-4766, American Chemical Society, USA 57(32), 4765-4766.
Hentschel Jendrik, Burnside Chloe, Mignot Ingrid, Leibundgut Marc, Boehringer Daniel, Ban Nenad (2017), The Complete Structure of the Mycobacterium smegmatis 70S Ribosome, in Cell Reports
, 20(1), 149-160.
Jomaa Ahmad, Boehringer Daniel, Leibundgut Marc, Ban Nenad (2016), Structures of the E. coli translating ribosome with SRP and its receptor and with the translocon, in Nature Communications
, 7(1), 10471-10471.
Bar-Yaacov Dan, Frumkin Idan, Yashiro Yuka, Chujo Takeshi, Ishigami Yuma, Chemla Yonatan, Blumberg Amit, Schlesinger Orr, Bieri Philipp, Greber Basil, Ban Nenad, Zarivach Raz, Alfonta Lital, Pilpel Yitzhak, Suzuki Tsutomu, Mishmar Dan (2016), Mitochondrial 16S rRNA Is Methylated by tRNA Methyltransferase TRMT61B in All Vertebrates, in PLOS Biology
, 14(9), e1002557-e1002557.
Greber Basil J., Ban Nenad (2016), Structure and Function of the Mitochondrial Ribosome, in Annual Review of Biochemistry
, 85(1), 103-132.
Greber Basil Johannes, Gerhardy Stefan, Leitner Alexander, Leibundgut Marc, Salem Michèle, Boehringer Daniel, Leulliot Nicolas, Aebersold Ruedi, Panse Vikram Govind, Ban Nenad (2016), Insertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation, in Cell
, 164(1-2), 91-102.
Aylett C. H. S., Sauer E., Imseng S., Boehringer D., Hall M. N., Ban N., Maier T. (2015), Architecture of human mTOR complex 1, in Science
, 351(6268), 48-52.
Ribosomes are responsible for mRNA directed protein synthesis in all organisms. Bacterial ribosomes are complex ribonucleoprotein assemblies that have molecular weights of approximately 2.5 million Daltons and contain 50-60 different protein molecules and three different RNAs. Eukaryotic ribosomes are approximately 30% larger than their bacterial counterparts with a molecular weight of at least 3.3 MDa (yeast). Although basic aspects of protein synthesis are preserved in all kingdoms of life, there are many important differences between bacterial and eukaryotic ribosomes and their roles in translation. Consequently, eukaryotic ribosomes require a large number of assembly and maturation factors during their biogenesis, they use numerous initiation factors, and are subjected to extensive regulation. The main goal of the research in my laboratory supported through Swiss National Science Foundation is to study structures of prokaryotic and eukaryotic ribosomes, ribosomal subunits and their complexes with various factors involved in protein synthesis. In our research we use a combination of biochemistry, crystallography and electron microscopy experiments. As evidenced by the list of recent publications from my lab we have accumulated the expertise and established the methodology to competitively conduct research in this field. As a continuation of our research on eukaryotic ribosomes we intend to study the mechanisms of ribosome assembly in yeast and the factors involved in this process, with a particular emphasis on the maturation of the eukaryotic 60S ribosomal subunit. Furthermore, we intend to investigate the mechanisms of translation initiation and reinitiation by special cellular messenger RNAs. Our recent results, obtained during the previous phase of this grant support, will provide the basis for this application.