Project

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Réplication et pathogenése des rhinovirus humains dans le contexte de co-infections avec d’autres virus respiratoires

English title Replication and pathogenesis of human rhinoviruses in the context of co- and successive infections with other respiratory viruses
Applicant Essaidi-Laziosi Manel
Number 158269
Funding scheme Marie Heim-Voegtlin grants
Research institution
Laboratoire de Virologie Service des Maladies Infectieuses Hôpitaux Universitaires de Genève
Dépt Microbiologie et Médecine Moléculaire Faculté de Médecine Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Medical Microbiology
Start/End 01.11.2015 - 28.02.2018
Approved amount 240'080.00
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All Disciplines (2)

Discipline
Medical Microbiology
Immunology, Immunopathology

Keywords (3)

pathogenesis; innate immunity; respiratory viruses

Lay Summary (French)

Lead
Réplication et pathogenése des rhinovirus humains dans le contexte de co-infections avec d’autres virus respiratoires
Lay summary
Les rhinovirus présentent la principale cause de rhume notamment chez les enfants et ont un impact économique considérable dû particulièrement à l’absentéisme, les visites médicales et la consommation de médicaments. Les rhinovirus causent généralement des maladies respiratoires peu graves. Toutefois, ils peuvent être associés à des cas d’exacerbation chez les patients immunosupprimés ou présentant des pathologies respiratoires comme l’asthme. Les rhinovirus co-circulent pendant l’année avec plusieurs autres virus respiratoires. De nombreuses études épidémiologiques ont suggéré des associations positives ou négatives entre ces différents virus. Cependant, le mécanisme expliquant ces associations reste peu clair.
Durant ce projet, j’envisage étudier la réplication des rhinovirus dans le contexte de co-infections et d’infections successives avec d’autres virus respiratoires dont le virus de la grippe, le coronavirus et le virus respiratoire syncytial… Dans ce contexte d’infections multiples, je suis intéressée par l’étude de la réponse immune innée potentiellement responsable du blocage ou de l’exacerbation de l’infection. Pour cela, des souches virales cliniques seront utilisées pour infecter des tissus de l’épithélium respiratoire humain reconstitué in vitro. Ce système de culture avait rendu possible l’amplification de souches virales cliniques incapables de pousser dans des lignées cellulaires standards (Tapparel et al., virology, 2013). Cette étude permettra, à court terme, d’élargir les connaissances sur la pathogénèse des différents virus respiratoires dans le contexte d’infections multiples ; et, à moyen et long termes, d’améliorer la gestion des patients à l’hôpital et d’éviter l’usage inapproprié de médicaments et/ou anti-viraux.
 
Direct link to Lay Summary Last update: 24.08.2015

Responsible applicant and co-applicants

Employees

Publications

Publication
Interferon-Dependent and Respiratory Virus-Specific Interference in Dual Infections of Airway Epithelia
Essaidi-Laziosi Manel, Geiser Johan, Huang Song, Constant Samuel, Kaiser Laurent, Tapparel Caroline (2020), Interferon-Dependent and Respiratory Virus-Specific Interference in Dual Infections of Airway Epithelia, in Scientific Reports, 10(1), 10246-10246.
Propagation of respiratory viruses in human airway epithelia reveals persistent virus-specific signatures
Manel Essaidi-Laziosi, Francisco Brito, Sacha Benaoudia, Léna Royston, Valeria Cagno, Mélanie Fernandes-Rocha, Isabelle Piuz, Evgeny Zdobnov, Song Huang, Samuel Constant, Marc-Olivier Boldi, Laurent Kaiser, Caroline Tappare (2017), Propagation of respiratory viruses in human airway epithelia reveals persistent virus-specific signatures, in JACI, S0091-6749(17), 1274-1285.
A new real-time RT-qPCR assay for the detection, subtyping and quantification of human respiratory syncytial viruses positive- and negative-sense RNAs.
Essaidi-Laziosi Manel, Lyon Matthieu, Mamin Aline, Fernandes Rocha Mélanie, Kaiser Laurent, Tapparel Caroline (2016), A new real-time RT-qPCR assay for the detection, subtyping and quantification of human respiratory syncytial viruses positive- and negative-sense RNAs., in Journal of virological methods, 235, 9-14.
Viral chimeras decrypt the role of enterovirus capsid proteins in viral tropism, acid sensitivity and optimal growth temperature
Lena Royston, Manel Essaidi-Laziosi, Francisco J. Pérez-Rodríguez, Isabelle Piuz, Johan Geiser, Karl-Heinz Krause, Song Huang, Samuel Constant, Laurent Kaiser, Dominique Garcin, Caroline Tapparel, Viral chimeras decrypt the role of enterovirus capsid proteins in viral tropism, acid sensitivity and optimal growth temperature, in PPAT, -(-), ---.

Collaboration

Group / person Country
Types of collaboration
Hopitaux universitaires Genève Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Epithelix SARL Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Europic 2016 Talk given at a conference DIFFERENTIAL PATHOGENESIS OF RHINOVIRUSES FROM SPECIES A, B AND C AND OF ENTEROVIRUS D68 IN HUMAN AIRWAY EPITHELIA 04.09.2016 Les diablerets, Switzerland Essaidi-Laziosi Manel;
74th Annual Swiss Society for Microbiology Meeting Talk given at a conference Differential pathogenesis of the most common respiratory viruses in reconstituted human airway epithelia 13.06.2016 Berne, Switzerland Essaidi-Laziosi Manel;
74th Annual Swiss Society for Microbiology Meeting Poster Respiratory viral infections in reconstituted human airway epithelia 13.06.2016 Berne, Switzerland Essaidi-Laziosi Manel;
6th Swiss Virology Meeting Poster Respiratory viral infections in reconstituted human airway epithelia 02.02.2016 Thun, Switzerland Essaidi-Laziosi Manel;


Associated projects

Number Title Start Funding scheme
178708 Pathogenesis of Human rhinoviruses in the context of multiple infection 01.03.2018 Marie Heim-Voegtlin grants

Abstract

Rhinoviruses (RVs) are the most frequent cause of the common cold and can be associated with disease of enhanced severity and infection of the lower respiratory tract. Rhinoviruses co-circulate with many other respiratory viruses in human populations but how they cross-interact is poorly understood. In this project, we intend to study RV replication and pathogenesis in the context of co- and successive infections with other human pathogens like influenza virus, respiratory syncytial virus and coronavirus. The interplay with cellular innate immune response will also be investigated. Infections will be performed in reconstituted human airway epithelia, a relevant model system widely used by my host laboratory to study RV biology. This study will extend the understanding of the pathogenesis of these highly common respiratory viruses as well as their pathogenesis in the context of multiple infections. This will improve patients handling in hospital settings and may avoid the misuse of medicines/antivirals.
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