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Lightsheet microscopy

English title Lightsheet microscopy
Applicant Affolter Markus
Number 157700
Funding scheme R'EQUIP
Research institution Abteilung Zellbiologie Biozentrum Universität Basel
Institution of higher education University of Basel - BS
Main discipline Cellular Biology, Cytology
Start/End 01.12.2014 - 30.11.2015
Approved amount 410'555.00
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All Disciplines (3)

Discipline
Cellular Biology, Cytology
Neurophysiology and Brain Research
Cardiovascular Research

Keywords (4)

microscopy; stem cells; angiogenesis; cell division

Lay Summary (German)

Lead
The visualisation of cellular and molecular processes as they occur in living systems has provided invaluable information regarding organ formation. Light sheet microscopy adds a new twist to live imaging, since it allows to visualise large specimens (for example entire zebrafish embryos) over extended periods of time. Such a light sheet microscope is not available at the Biozentrum yet, but the grant now allow to purchase such a system. Many research groups of the Biozentrum will use this new instrument, which will be placed in the Imaging Facility.
Lay summary
Die Mikroskopie hat in den letzten Jahren eine zentrale Rolle in der Biologie gespielt. Dies vor allem dank neuen fluoreszierenden Proteinen, die es erlauben, zelluläre und molekulare Prozesse direkt in lebendigen Organsimen zu visualisieren. Mit Hilfe von neuen Mikroskopen, so zum Beispiel dem "Light Sheet Microscope", lassen sich ganze Embryonen mit geringer Phototoxizität beobachten. Dieser Grant erlaupt es nun, diese neue Technologie am Biozentrum einzuzrichten.
Direct link to Lay Summary Last update: 11.11.2014

Responsible applicant and co-applicants

Associated projects

Number Title Start Funding scheme
149641 Control of centrosome duplication and chromosome segregation 01.12.2013 Project funding (Div. I-III)
140944 Molecular Mechanisms of Neuronal Synapse Formation 01.08.2012 Project funding (Div. I-III)
125759 NCCR SYNAPSY: The synaptic bases of mental diseases (phase I) 01.10.2010 National Centres of Competence in Research (NCCRs)
133658 Cellular and molecular mechanism of asymmetric stem cell division 01.08.2011 SNSF Professorships
156838 In vivo cell biology of organ morphogenesis 01.12.2014 Project funding (Div. I-III)

Abstract

Research at the Biozentrum of the University of Basel focuses on basic cellular and molecular aspects of living systems. The recent technological developments in Lightsheet microscopy have opened up new possibilities for light microscopy-based live-cell imaging of whole organisms or cells. To install this novel technology at the Biozentrum, we plan to purchase an instrument for Light sheet microscopy. This acquisition will be embedded within the new imaging core facility set up to provide state-of-the-art microscopy technology with the necessary support and training to the Biozentrum research community.
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