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Mechanisms of phage resistance in Erwinia amylovora

English title Mechanisms of phage resistance in Erwinia amylovora
Applicant Fieseler Lars
Number 156947
Funding scheme Project funding
Research institution Zürcher Hochschule für Angewandte Wissenschaften
Institution of higher education Zurich University of Applied Sciences - ZHAW
Main discipline Experimental Microbiology
Start/End 01.11.2014 - 30.06.2018
Approved amount 732'740.00
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All Disciplines (3)

Discipline
Experimental Microbiology
Molecular Biology
Ecology

Keywords (5)

bacteriophage; phage resistance; receptor; genome plasticity; Erwinia amylovora

Lay Summary (German)

Lead
Bakterieninfizierenden Viren, Bakteriophagen, können eingesetzt werden, um pathogene Bakterien zu eliminieren. Jedoch können Bakterien Resistenzen gegenüber Bakteriophagen entwickeln. Dieses Projekt soll klären, ob die Entwicklung von Phagenresistenzen in Erwinia amylovora verhindert werden kann.
Lay summary

Mechanismen der Phagenresistenzentwicklung in Erwinia amylovora

Développement de méchanismes de résistance de Erwinia amylovora contre bacteriophages

Sviluppo di meccanismi di resistenza di  Erwinia amylovora contro i bacteriofagi

 

Lead

Bakterieninfizierenden Viren, Bakteriophagen, können eingesetzt werden, um pathogene Bakterien zu eliminieren. Jedoch können Bakterien Resistenzen gegenüber Bakteriophagen entwickeln. Dieses Projekt soll klären, ob die Entwicklung von Phagenresistenzen in Erwinia amylovora verhindert werden kann.

 

Inhalt und Ziele des Forschungsprojektes 

Der Einsatz von Bakteriophagen zur Kontrolle pathogener Bakterien ist eine gute Alternative zum Einsatz konventioneller Antibiotika. Trotz der vielen Vorteile, die diese Art der biologischen Schädlingsbekämpfung mit sich bringt, können Bakterien resistent gegenüber einer Phageninfektion werden. Die Mechanismen zur Ausbildung solcher Resistenzen und der Entstehung von Kreuzresistenzen sind vielfälltig, aber in Erwinia amylovora, dem Erreger des Feuerbrandes in Kernobstgewächsen, noch nicht ausreichend verstanden.

In diesem Projekt identifizieren wir Faktoren, die an der Entstehung einer Phagenresistenz beteiligt sind. Darüber hinaus untersuchen wir, wie die Phagenbehandlung angepasst werden kann, um die Ausbildung von Resistenzen zu minimieren. Um die Auswirkungen einer Phagenresistenz besser verstehen zu können, untersuchen wir auch die Anpassungen und Fitness resistenter Bakterien in ihrem natürlichen Lebensraum.     

 

Wissenschaftlicher und gesellschaftlicher Kontext des Forschungsprojektes

Dieses Grundlagenforschungsprojekt soll zeigen, ob die Bedenken hinsichtlich  Resistenzentwicklungen bei einer Phagentherapie gerechtfertigt sind.

Direct link to Lay Summary Last update: 14.10.2014

Responsible applicant and co-applicants

Employees

Publications

Publication
Complete Genome Sequences of Erwinia amylovora Phages vB_EamP-S2 and vB_EamM-Bue1
Knecht Leandra E., Born Yannick, Pothier Joël F., Loessner Martin J., Fieseler Lars (2018), Complete Genome Sequences of Erwinia amylovora Phages vB_EamP-S2 and vB_EamM-Bue1, in Microbiology Resource Announcements, 7(3).
Erwinia amylovora phage vB_EamM_Y3 represents another lineage of hairy Myoviridae
Buttimer Colin, Born Yannick, Lucid Alan, Loessner Martin J., Fieseler Lars, Coffey Aidan (2018), Erwinia amylovora phage vB_EamM_Y3 represents another lineage of hairy Myoviridae, in Research in Microbiology.
Structural and functional diversity in Listeria cell wall teichoic acids
Shen Y, Boulos S, Sumrall E, Gerber B, Julian-Rodero A, Eugster MR, Fieseler L, Nyström L, Ebert MO, Loessner MJ (2017), Structural and functional diversity in Listeria cell wall teichoic acids, in J Biol Chem, 17832.
Genome sequences of five nonvirulent Listeria monocytogenes Serovar 4 Strains
Sumrall E, Klumpp J, Shen Y, Loessner MJ (2016), Genome sequences of five nonvirulent Listeria monocytogenes Serovar 4 Strains, in Genome Announcements, e00179-16.
Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds
Born Y, Bosshard L, Duffy B, Loessner MJ, Fieseler L (2015), Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds, in Bacteriophage, e1074330.

Collaboration

Group / person Country
Types of collaboration
Zurich University of Applied Sciences, Dr. Theo Smits Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel
Agroscope, Dr. Cosima Pelludat Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel
ETH Zurich, Prof. Dr. Martin Loessner Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
SSM Annual Meeting Talk given at a conference Mutations in the bcs operon of Erwinia amylovora abolish infection by bacteriophages S6 and M7. 28.08.2018 Lausanne, Switzerland Knecht Leandra; Fieseler Lars;
Viruses of Microbes Conference Poster Mutations in the bcs operon abolish infection by bacteriophages S6 and M7 in Erwinia amylovora. 09.07.2018 Wroclaw, Poland Fieseler Lars; Knecht Leandra;
Viruses of Microbes Conference Talk given at a conference The phage encoded depolymerase DpoL1 causes a strong synergistic inhibitory effect on Erwinia amylovora if applied with capsule independent phages. 09.07.2018 Wroclaw, Poland Fieseler Lars; Knecht Leandra; Born Yannick;
SGLH Jahrestagung Talk given at a conference Entwicklung eines Lux‐Reporterphagen zum Nachweis von Erwinia amylovora 22.06.2017 Zürich, Switzerland Born Yannick; Fieseler Lars; Loessner Martin;
Bacteriophages Talk given at a conference The effectiveness of T5-like phages to control Salmonella enterica 13.10.2016 Moskau, Russia Fieseler Lars; Born Yannick;
Viruses of Microbes Conference Poster Identification of phage receptors in Erwinia amylovora after transposon mutagenesis 18.07.2016 Liverpool, Great Britain and Northern Ireland Loessner Martin; Fieseler Lars; Knecht Leandra; Born Yannick;
Viruses of Microbes Conference Talk given at a conference Engineered phages for efficient control and rapid detection of viable Erwinia amylovora cells 18.07.2016 Liverpool, Great Britain and Northern Ireland Loessner Martin; Born Yannick; Fieseler Lars;
21st biennial Evergreen International Phage Meeting Poster A bacteriophage cocktail to control Salmonella Typhimurium 02.08.2015 Olympia, United States of America Born Yannick; Fieseler Lars;
21st biennual Evergreen International Phage Meeting Poster Implications of the phage depolymerase DpoL1 on Erwinia amylovora 02.08.2015 Olympia, United States of America Fieseler Lars; Born Yannick;


Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Feuerbrand-Fünf-Länder-Treffen Talk 07.11.2017 Bad Waldsee, Germany Born Yannick; Loessner Martin; Fieseler Lars; Knecht Leandra;
Koordinationssitzung «Gemeinsam gegen Feuerbrand» Talk 03.11.2017 Wädenswil, Switzerland Fieseler Lars; Born Yannick;
Lebensmitteltagung Talk 17.11.2016 Wädenswil, Switzerland Fieseler Lars;
Lebensmitteltagung Talk 17.11.2016 Wädenswil, Switzerland Loessner Martin; Fieseler Lars; Born Yannick;
Gemeinsam gegen Feuerbrand. 1. Nationale Tagung. Talk 17.11.2015 Wädenswil, Switzerland Knecht Leandra; Born Yannick; Loessner Martin; Fieseler Lars;


Communication with the public

Communication Title Media Place Year
Media relations: radio, television Interview im ORF ORF International 2017

Abstract

Bacteriophages have regained much attention as suitable bio control agents in food and agriculture and represent a useful tool to inhibit pathogenic bacteria. However, phage resistance can emerge shortly after phage exposure. Although the rate of resistance development can be lowered using phage cocktails, resistant bacteria can still arise, which is a major concern about phage therapy worldwide. Moreover, it remains unclear to which extent “multiple phage resistant bacteria” can develop. In the fire blight pathogen E. amylovora phage insensitive bacteria emerge under constant selective pressure. Therefore, we aim to investigate the molecular details of phage adsorption, analyze resistance development, explain the mechanisms which provoke transient and permanent phage resistance, and assess the impact of bacterial resistance on virulence and competitive fitness of the host bacterium. Finally, the impact of resistance development will be analyzed to demonstrate if resistant bacteria can compete with wild-type bacteria and if they are able to persist and display full virulence in the environment. We will clarify if concerns about phage therapy are justified and if phage as a bio control agent can meet the requirements of a reliable alternative to conventional control methods.
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