mtrABC; Shewanella oneidensis MR-1; photovoltaics; protein engineering; electron conduit; cyanobacteria; photosynthesis; mtrA; mtrB; mtrC; multiheme cytochrome c; extracellular electron transfer; cytochrome c machinery (ccm)
Gillen Alice J., Siefman Daniel J., Wu Shang-Jung, Bourmaud Claire, Lambert Benjamin, Boghossian Ardemis A. (2020), Templating colloidal sieves for tuning nanotube surface interactions and optical sensor responses, in Journal of Colloid and Interface Science
, 565, 55-62.
Reggente Melania, Politi Sara, Antonucci Alessandra, Tamburri Emanuela, Boghossian Ardemis A. (2020), Design of Optimized PEDOT‐Based Electrodes for Enhancing Performance of Living Photovoltaics Based on Phototropic Bacteria, in Advanced Materials Technologies
, 5(3), 1900931-1900931.
Wu Shang-Jung, Boghossian Ardemis A. (2019), Analytical Approaches for Monitoring DNA–Protein Interactions, in CHIMIA International Journal for Chemistry
, 73(4), 283-287.
Zubkovs Vitalijs, Antonucci Alessandra, Schuergers Nils, Lambert Benjamin, Latini Andrea, Ceccarelli Raino, Santinelli Andrea, Rogov Andrii, Ciepielewski Daniel, Boghossian Ardemis A. (2018), Spinning-disc confocal microscopy in the second near-infrared window (NIR-II), in Scientific Reports
, 8(1), 13770-13770.
Gillen Alice J., Kupis-Rozmysłowicz Justyna, Gigli Carlo, Schuergers Nils, Boghossian Ardemis A. (2018), Xeno Nucleic Acid Nanosensors for Enhanced Stability Against Ion-Induced Perturbations, in The Journal of Physical Chemistry Letters
, 9(15), 4336-4343.
Wu Shang-Jung, Schuergers Nils, Lin Kun-Han, Gillen Alice, Corminboeuf Clemence, Boghossian Ardemis (2018), Restriction Enzyme Analysis of Double-Stranded DNA on Pristine Single-Walled Carbon Nanotubes, in ACS Applied Materials & Interfaces
Zubkovs Vitalijs, Schuergers Nils, Lambert Benjamin, Ahunbay Esra, Boghossian Ardemis A. (2017), Mediatorless, Reversible Optical Nanosensor Enabled through Enzymatic Pocket Doping, in Small
, 13(42), 1701654-1701654.
Schuergers Nils, Werlang Caroline, Ajo-Franklin Caroline, Boghossian Ardemis (2017), A synthetic biology approach to engineering living photovoltaics
, Ecole Polytechnique Federale de Lausanne (EPFL), Energy & Environmental Science.
Antonucci Alessandra, Kupis-Rozmysłowicz Justyna, Boghossian Ardemis A. (2017), Noncovalent Protein and Peptide Functionalization of Single-Walled Carbon Nanotubes for Biodelivery and Optical Sensing Applications, in ACS Applied Materials & Interfaces
, 9(13), 11321-11331.
Biological systems offer inherent advantages in fault tolerance, regeneration, abundance, and energy storage ideally suited for photovoltaic (PV) applications. Despite promising maximum theoretical efficiencies, biological PVs remain an underdeveloped technology hindered by poor electron extraction mechanisms that limit efficiencies. The focus of this proposal is to engineer a recombinant, light-harvesting organism capable of exporting photosynthetically derived electrons for solar energy use. The proposed approach is to couple the electron-exporting mechanism in Shewanella with the light-harvesting mechanism in cyanobacteria to create a recombinant, living cell capable of exporting electrons derived from absorbing light to split water. This approach entails the heterologous expression and optimization of the three proteins, mtrA, mtrB, and mtrC, responsible for exporting electrons in Shewanella in cyanobacteria. Through synthetic biology and protein engineering, this project focuses on engineering and optimizing the interface between the synthetic Shewanella electron conduit and the light-harvesting, cyanobacterial system.