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Exploring the interface between ribosome assembly and nucleo-cytoplasmic transport

English title Exploring the interface between ribosome assembly and nucleo-cytoplasmic transport
Applicant Panse Vikram
Number 152995
Funding scheme SNSF Professorships
Research institution Institut für Medizinische Mikrobiologie Universität Zürich
Institution of higher education University of Zurich - ZH
Main discipline Biochemistry
Start/End 01.08.2014 - 30.09.2016
Approved amount 951'202.00
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Keywords (3)

Nuclear transport; Ribosome biogenesis; Ribosome export

Lay Summary (German)

Lead
Exploring the interface between nucleocytoplasmic transport machinery and ribosome assembly
Lay summary

 

Eukaryotische Ribosomen- Montage ist ein spektakuläres Beispiel für eine hochdynamische und geregelten Prozess, der in verschiedenen zellulären Gebieten auftritt : der Nukleolus , Kernplasma und das Zytoplasma. Dieses Verfahren schlecht verstanden , die in der Nukleolus beginnt , erfordert > 350 konserviert vorübergehend Zuordnung Montage Faktoren , deren site (s) von Aktion und Funktion (en) auf reif Pre-ribosomalen Partikel verbleiben , entdeckt zu werden. Ferner ist die eukaryotische Ribosomen- Biogenese eng mit der nucleaocytoplasmic Transportmaschinen verbunden sind: die Kernimportmaschineriesorgt für eine sichere Lieferung von ribosomalen Proteinen zu entwickeln pre- ribosomalen Partikel in der Nukleolus , dem Ort der Biogenese und der Kernexport Maschinen erleichtert den Transport von Pre- ribosomalen Partikel durch Kernporenkomplexein das Zytoplasma , dem Ort der Funktion.

Durch die Nutzung von Ansätzen zugänglich im Modellorganismus Bäckerhefe zu entdecken :

(1) wie die zellulären Transport-Maschinen -Schnittstellen mit dem Ribosom Montage Weg.

(2) molekulare Umgebung von Montage Faktoren auf pre- ribosomalen Partikel.

Gemeinsam werden wir Prinzipien, die die Bau-und Transport dieser universellen Übersetzungsgerät regeln offenbaren, und möglicherweise in einem Zusammenhang makromolekularen Anordnungen.
Direct link to Lay Summary Last update: 12.03.2014

Responsible applicant and co-applicants

Employees

Publications

Publication
DSS1/Sem1, a Multifunctional and Intrinsically Disordered Protein.
kragelund birthe, Schenstrøm Sgine, Panse Vikram, Hartmann-Petersen Rasmus (2016), DSS1/Sem1, a Multifunctional and Intrinsically Disordered Protein., in Trends Biochem Sci., 41(5), 446-459.
Insertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation.
Greber Basil, Gerhardy Stefan, Leitner Alexander, Leibundgut Marc, Salem Michele, Boehringer Daniel, Leillloit Nicolas, Aebersold Rudolf, Panse Vikram, Ban Nenad (2016), Insertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation., in Cell, 164(1-2), 91-102.
A non-canonical pathway for Crm1-export complex assembly.
Panse vikram, Fischer Ute, Schütz Sabina, ALtvater Martin, Faza Marius Boulos (2015), A non-canonical pathway for Crm1-export complex assembly., in Elife, 10.7554(eLife.0347), 1-16.
A new system for naming ribosomal proteins.
Ban Nenad, Beckmann Roland, Cate Jamie H D, Dinman Jonathan D, Dragon François, Ellis Steven R, Lafontaine Denis L J, Lindahl Lasse, Liljas Anders, Lipton Jeffrey M, McAlear Michael A, Moore Peter B, Noller Harry F, Ortega Joaquin, Panse Vikram Govind, Ramakrishnan V, Spahn Christian M T, Steitz Thomas A, Tchorzewski Marek, Tollervey David, Warren Alan J, Williamson James R, Wilson Daniel, Yonath Ada, Yusupov Marat (2014), A new system for naming ribosomal proteins., in Current opinion in structural biology, 24, 165-9.
A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly.
Schütz Sabina, Fischer Ute, Altvater Martin, Nerurkar Purnima, Peña Cohue, Gerber Michaela, Chang Yiming, Caesar Stefanie, Schubert Olga T, Schlenstedt Gabriel, Panse Vikram G (2014), A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly., in eLife, 3, 03473-03473.
Assembly and nuclear export of pre-ribosomal particles in budding yeast.
Gerhardy Stefan, Menet Anna Maria, Peña Cohue, Petkowski Janusz Jurand, Panse Vikram Govind (2014), Assembly and nuclear export of pre-ribosomal particles in budding yeast., in Chromosoma, 123(4), 327-44.
Dissecting ribosome assembly and transport in budding yeast.
Altvater Martin, Schütz Sabina, Chang Yiming, Panse Vikram Govind (2014), Dissecting ribosome assembly and transport in budding yeast., in Methods in cell biology, 122, 437-61.
Eukaryotic ribosome assembly and nuclear export.
Nerurkar Purnima, Altvater Martin, Gerhardy Stefan, Schütz Sabina, Fischer Ute, Weirich Christine, Panse Vikram, Eukaryotic ribosome assembly and nuclear export., in International review of Cell and Molecular Biology , NA(NA), 1-25.
Ribosome assembly and export.
Panse Vikram, Weirich Christine, Ribosome assembly and export., in Encyclopedia of Cell Biology, NA(NA), 1-25.

Collaboration

Group / person Country
Types of collaboration
ETH Zurich Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure

Associated projects

Number Title Start Funding scheme
128405 Dissecting the biogenesis of eukaryotic ribosomal subunits 01.08.2010 SNSF Professorships
166571 Exploring the interface between ribosome assembly and nucleocytoplasmic transport 01.10.2016 Project funding (Div. I-III)

Abstract

Eukaryotic ribosome assembly is a spectacular example of a highly dynamic and regulated process that occurs in different cellular territories: the nucleus, nucleoplasm and the cytoplasm. This poorly understood process, which begins in the nucleolus, requires >350 conserved transiently associating assembly factors, whose site(s) of action and function(s) on maturing pre-ribosomal particles remain to be discovered. Further, the eukaryotic ribosome biogenesis is intimately linked with the nucleaocytoplasmic transport machinery: the nuclear import machinery ensures safe delivery of ribosomal proteins to evolving pre-ribosomal particles in the nucleolus, the site of biogenesis, and the nuclear export machinery facilitates transport of pre-ribosomal particles through nuclear pore complexes into the cytoplasm, the site of function.By exploiting approaches amenable in the model organism budding yeast in combination with in vitro biochemistry, targeted proteomics and structural approaches, we will uncover:(1) how the cellular transport machinery interfaces with the ribosome assembly pathway. (2) molecular environment of assembly factors on pre-ribosomal particles.Together, we will reveal principles that govern the construction and transport of this universal translating machine, and potentially related macromolecular assemblies.
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