Endospores; Extremophiles; Geothermal activity; Genomics ; Heavy and trace metal tolerance
Filippidou Sevasti, Wunderlin Tina, Junier Thomas, Jeanneret Nicole, Dorador Cristina, Molina Veronica, Johnson David R., Junier Pilar (2016), A Combination of Extreme Environmental Conditions Favor the Prevalence of Endospore-Forming Firmicutes, in Frontiers in Microbiology
, 7, 1707.
Filippidou Sevasti, Jaussi Marion, Junier Thomas, Wunderlin Tina, Jeanneret Nicole, Palmieri Fabio, Palmieri Ilona, Roussel-Delif Ludovic, Vieth-Hillebrand Andrea, Vetter Alexandra, Chain Patrick S., Regenspurg Simona, Junier Pilar (2016), Description of Anoxybacillus geothermalis sp. nov., an aerobic endospore-forming bacterium isolated from mineral deposits in the Groß Schönebeck geothermal station, Germany, in International Journal of Systematic and Evolutionary Microbiology
, 66, 2944-2951.
Matthieu Bueche, Junier Pilar (2016), Effect of organic carbon and metal accumulation on the bacterial communities in sulphidogenic sediments, in Environmental Science and Pollution Research
, 23(11), 10443-10456.
Ganesan Sathiyanarayanan, Filippidou Sevasti, Junier Thomas, Muñoz-Rufatt Patricio, Jeanneret Nicole, Wunderlin Tina, Sieber Nathalie, Dorador Cristina, Junier Pilar (2016), Manganese-II oxidation and Copper-II resistance in endospore forming Firmicutes isolated from uncontaminated environmental sites, in AIMS Environmental Science
, 3(2), 220-238.
Wunderlin Tina, Junier Thomas, Paul Christophe, Jeanneret Nicole, Junier Pilar (2016), Physical isolation of endospores from environmental samples by targeted lysis of vegetative cells, in Journal of Visual Experiments
, 107, e53411.
Filippidou Sevasti, Jaussi Marion, Junier Thomas, Wunderlin Tina, Jeanneret Nicole, Regenspurg Simona, Li Po-E, Lo Chien-Chi, Johnson Shannon, McMurry Kim, Gleasner Cheryl D, Vuyisich Momchilo, Chain Patrick S, Junier Pilar (2015), Genome Sequence of Aeribacillus pallidus Strain GS3372, an Endospore-Forming Bacterium Isolated in a Deep Geothermal Reservoir., in Genome announcements
, 3(4), e00982-15.
Filippidou Sevasti, Jaussi Marion, Junier Thomas, Wunderlin Tina, Roussel-Delif Ludovic, Jeanneret Nicole, Vieth-Hillebrand Andrea, Vetter Alexandra, Regenspurg Simona, Johnson Shannon L, McMurry Kim, Gleasner Cheryl D, Lo Chien-Chi, Li Paul, Vuyisich Momchilo, Chain Patrick S, Junier Pilar (2015), Genome Sequence of Anoxybacillus geothermalis Strain GSsed3, a Novel Thermophilic Endospore-Forming Species., in Genome announcements
, 3(3), e00575-15.
Filippidou Sevasti, Wunderlin Tina, Junier Thomas, Jeanneret Nicole, Johnson Shannon, McMurry Kim, Gleasner Cheryl D, Lo Chien-Chi, Li Po-E, Vuyisich Momchilo, Chain Patrick S, Junier Pilar (2015), Genome Sequence of Bacillus alveayuensis Strain 24KAM51, a Halotolerant Thermophile Isolated from a Hydrothermal Vent., in Genome announcements
, 3(4), e00981-15.
Bergottini Veronica M, Filippidou Sevasti, Junier Thomas, Johnson Shannon, Chain Patrick S, Otegui Monica B, Zapata Pedro D, Junier Pilar (2015), Genome Sequence of Kosakonia radicincitans Strain YD4, a Plant Growth-Promoting Rhizobacterium Isolated from Yerba Mate (Ilex paraguariensis St. Hill.)., in Genome announcements
, 3(2), e00239-15.
Junier Thomas, Hervé Vincent, Wunderlin Tina, Junier Pilar (2015), MLgsc: A Maximum-Likelihood General Sequence Classifier., in PloS one
, 10(7), 0129384-0129384.
Filippidou Sevasti, Junier Thomas, Wunderlin Tina, Lo Chien-Chi, Li Po-E, Chain Patrick S, Junier Pilar (2015), Under-detection of endospore-forming Firmicutes in metagenomic data., in Computational and structural biotechnology journal
, 13, 299-306.
Endospore-forming bacteria (EFB) are a paraphyletic group of Gram-positive bacteria belonging to the phylum Firmicutes. Some are well known as human pathogens (e.g. Bacilllus anthracis or Clostridium botulinum), but much less studied from an ecological point of view. Until now, and based mainly on culturing, EFB were considered one of the best-characterized microbial groups (over 90 representative genera). However during the SNF project “Metabolic capabilities of spore-forming bacteria” (FN 31003A-132358) we have developed methodological approaches that have shown that this group is currently overlooked by culture-independent approaches. We have identified genetic markers that can be used to study EFB directly from environmental samples without cultivation. In particular the gene encoding for the master regulator of the genetic cascade leading to the formation of endospores (spo0A) has shown to be present in all and is exclusive to EFB. This gene was used to develop culture-independent approaches for the phylogenetic assessment of EFB communities, as well for the quantification of EFB in complex microbial communities. These methods have been applied to evaluate our overall hypothesis that endospore formation is a survival strategy favored in extreme environments. In this context we have shown for the first time a correlation of EFB and environmental hazards such as elevated heavy and trace metals (HTM) content in sediments and lake eutrophication. We have also carried out a global survey of EFB prevalence (relative to total bacteria) for environments with high salt content, exposed to UV radiation and desiccation, as well as natural and manmade geothermal sites (specific range of electron donors/acceptors, temperature and pH conditions). This global survey indicates that EFB are indeed favored under extreme environmental conditions. The molecular study has been complemented by an effort to culture and characterize new EFB strains. We have identified close to 190 strains and several of those present a natural resistance to stress, including the tolerance to copper and manganese, as well as salinity and acidic pH.In summary all our preliminary results suggest that EFB are adapted to stress factors and therefore the aim of this project is to advance in our understanding of the determinants (genetic and physiological) for these adaptations. For this we propose to combine various approaches including comparative genomics, expression analysis, single-cell genomics, metagenomics, and experimental validation to answer the following specific questions: are EFB favored in other HTM-impacted environments? What are the mechanisms of HTM tolerance and immobilization found in EFB? What are the specific adaptations of EFB to oligotrophic environments? The expected outcome of this project is on the one hand to discover new genes/proteins involved in HTM tolerance that can have a biotechnological application, and on the other to discover entire new metabolic pathways never reported for this bacterial group.