exodermis; strigolactone; vacuole; membrane transport; auxin conjugates; hypodermal passage cell
Bombarely Aureliano, Moser Michel, Amrad Avichai, Bao Manzhu, Bapaume Laure, Barry Cornelius S, Bliek Mattijs, Boersma Maaike R, Borghi Lorenzo, Bruggmann Rémy, Bucher Marcel, D'Agostino Nunzio, Davies Kevin, Druege Uwe, Dudareva Natalia, Egea-Cortines Marcos, Delledonne Massimo, Fernandez-Pozo Noe, Franken Philipp, Grandont Laurie, Heslop-Harrison J S, Hintzsche Jennifer, Johns Mitrick, Koes Ronald, Lv Xiaodan (2016), Insight into the evolution of the Solanaceae from the parental genomes of Petunia hybrida., in Nature plants
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Sasse Joëlle, Schlegel Markus, Borghi Lorenzo, Ullrich Friederike, Lee Miyoung, Liu Guo-Wei, Giner José-Luis, Kayser Oliver, Bigler Laurent, Martinoia Enrico, Kretzschmar Tobias (2016), Petunia hybrida PDR2 is involved in herbivore defense by controlling steroidal contents in trichomes., in Plant, cell & environment
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Hwang Jae-Ung, Song Won-Yong, Hong Daewoong, Ko Donghwi, Yamaoka Yasuyo, Jang Sunghoon, Yim Sojeong, Lee Eunjung, Khare Deepa, Kim Kyungyoon, Palmgren Michael, Yoon Hwan Su, Martinoia Enrico, Lee Youngsook (2016), Plant ABC Transporters Enable Many Unique Aspects of a Terrestrial Plant's Lifestyle., in Molecular plant
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Borghi Lorenzo, Liu Guo-Wei, Emonet Aurélia, Kretzschmar Tobias, Martinoia Enrico (2016), The importance of strigolactone transport regulation for symbiotic signaling and shoot branching., in Planta
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Kang Joohyun, Yim Sojeong, Choi Hyunju, Kim Areum, Lee Keun Pyo, Lopez-Molina Louis, Martinoia Enrico, Lee Youngsook (2015), Abscisic acid transporters cooperate to control seed germination., in Nature Communication
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Sasse Joelle, Simon Sibu, Gübeli Christian, Liu Guo-Wei, Cheng Xi, Friml Jiri, Bouwmeester Harro, Martinoia Enrico, Borghi Lorenzo (2015), Asymmetric localizations of the ABC transporter PaPDR1 trace paths of directional strigolactone transport., in Current Biology
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Xu Haoxing, Martinoia Enrico, Szabo Ildiko (2015), Organellar channels and transporters., in Cell calcium
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Borghi Lorenzo, Kang Joohyun, Ko Donghwi, Lee Youngsook, Martinoia Enrico (2015), The role of ABCG-type ABC transporters in phytohormone transport., in Biochem. Soc. Trans
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Ko Donghwi, Kang Joohyun, Kiba Takatoshi, Park Jiyoung, Kojima Mikiko, Do Jihye, Kim Kyung Yoon, Kwon Mi, Endler Anne, Song Won-Yong, Martinoia Enrico, Sakakibara Hitoshi, Lee Youngsook (2014), Arabidopsis ABCG14 is essential for the root-to-shoot translocation of cytokinin, in Proc. Natl. Acad, Sci. USA
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SummaryStrigolactones are now well-established plant hormones. Excreted to the soil they induce the branching of mycorrhizal fungi, strongly promoting mycorrhization. Within the plant, together with auxin they are involved in the control of shoot branching. Furthermore, during the last years it was recognized that strigolactones play many other roles in controlling diverse aspects of plant development. Recently we succeeded in identifying an ABC transporter, PhPDR1 which acts as the strigolactone exporter in Petunia. Petunia plants missing PhPDR1show a strongly reduced mycorrhization and increase lateral branch formation. Recent, unpublished results from our laboratory revealed that PhPDR1 is polarly localized in root tip cells and in hypodermal passage cells (HPCs), the cells of the root exodermis responsible for strigolactone exudation and at the same time entry points for mycorrhizal funghi. However, despite our discovery of PhPDR1, many questions concerning strigolactone transport remain open and so far our knowledge about HPCs and the root exodermis is limited. In a related project in our laboratory aimed to characterize phytohormone transport, we realized that transport of phytohormone conjugates is a neglected topic, despite the potential important role these conjugates play. The hereby submitted project proposal integrates three projects. Within subproject A we would like to continue and expand our work on strigolactone transport. We will investigate the tissue and subcellular localization of PhPDR1 in the nodes, where PhPDR1 expression has been demonstrated, and investigate the crosstalk between PhPDR1 and PIN1, an auxin transporter, which control bud dormancy or outgrowth. Within this subproject we will also make an effort to identify and characterize a strigolactone importer. In subproject B, which is a ”spin-off” of subproject A, we plan to analyze in detail HPC function, since the presence of the strigolactone transporter in HPCs raised our interest in this cell type, which is only barely explored. We will perform a forward genetic screen to identify mutants with impaired HPCs and/or exodermis. In a second, hypothesis-based approach we will explore the possibility whether the recently discovered ESB1 and ESB1-like proteins required for endodermal Casparian strip formation are also involved in the formation of Casparian strips in the exodermis. In subproject C we would like to characterize the vacuolar transporters for indoyl acetic acid-amino acid and -glucose conjugates and investigate how plants react when these, partially unstable conjugates cannot be delivered to the vacuole. In conclusion, although the three subprojects are quite diverse, they have a common theme: phytohormone transport and the characterization of cells that play an important role in this transport.