Fluorescence Lifetime Imaging (FLIM); Fluorescence Resonance Energy Transfer (FRET); Molecular Interactions
Ummadisingu Amita, Steier Ludmilla, Seo Ji-Youn, Matsui Taisuke, Abate Antonio, Tress Wolfgang, Grätzel Michael (2017), The effect of illumination on the formation of metal halide perovskite films, in Nature
, 545(7653), 208-212.
The ability to label specific sub-cellular structures or proteins of interest in combination with the visualization of live specimens using fluorescence light microscopy or laser-scanning confocal microscopy has revolutionized research in life-sciences over the past three decades. However, fluorescence microcopy is diffraction limited and even recent super resolution methods do not allow investigating physical interactions of fluorescently labeled structures. Fluorescence Resonance Energy transfer (FRET) is an imaging approach which allows deciphering the interactome of proteins in living model systems. But this requires the precise measurement of FRET efficiencies which is only reliably possible via Fluorescence Lifetime Imaging (FLIM).The objective of this R’Equip grant application is to request funding for a dedicated Fluorescence Lifetime Imaging (FLIM) Microscope that will be hosted and maintained at the BioImaging and Optics platform (PT-BIOP) of the EPFL. This novel imaging capability will contribute to gaining new, important insights of proprotein convertases and their role in cancer biology (PI: D. Constam) as well as the investigation of post translational protein modifications (PI: G. van der Goot).Beyond the specific applications detailed in this research proposal, additional principal investigators including Prof. K. Johnsson, Prof S. Manley and Prof. P. Goenczy, have expressed a strong interest in using this technology. We anticipate that the availability of this state-of-the-art microscope for the entire EPFL community will foster the development of a number of new additional projects in the near future.