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Nanoparticles: Surface, protein & cell interaction

English title Nanoparticles: Surface, protein & cell interaction
Applicant Petri-Fink Alke
Number 150737
Funding scheme SNSF Professorships
Research institution Département de Chimie Université de Fribourg
Institution of higher education University of Fribourg - FR
Main discipline Inorganic Chemistry
Start/End 01.10.2013 - 31.12.2015
Approved amount 457'425.00
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All Disciplines (2)

Discipline
Inorganic Chemistry
Material Sciences

Keywords (5)

Protein corona; Cell interactions; Nanoparticles; Nanorods; Mono-end functionalization

Lay Summary (German)

Lead
Nanopartikel haben ein hohes Anwendungspotential in der Medizin. Um dieses Potential möglichst stark zu nutzen, muss das Verhalten von Nanopartikeln im biologischen Umfeld besser verstanden werden. Bisher wurde stark auf die Wechselwirkung von Nanopartikeln mit Proteinen eingegangen. Es ist jedoch nicht klar, was mit diesen Proteinen geschieht, wenn Partikel von Zellen internalisiert werden.
Lay summary

Inhalt des Forschungsprojekts

Es ist unser Ziel, die Wechselwirkungen zwischen einfachen und chemisch komplexeren Nanopartikeln mit biologischen Molekülen besser zu verstehen, um diese Materialien für die Nanomedizin zugänglich zu machen. In vergangenen Projekten haben wir, abhängig von Partikel-Typ, Grösse und Oberfläche, Proteine identifiziert, die sich über die Zeit im biologischen Medium an die Partikeloberfläche anlagern. In diesem Projekt werden wir Fragen beantworten, wie beispielsweise: “Bleiben diese Proteine an der Partikeloberfläche, wenn diese Partikel von Zellen aufgenommen werden?”, oder “Mit was werden diese Proteine ausgetauscht und welche Konsequenzen ergeben sich daraus für die Partikel und die Zelle?” bzw. “Wie sieht die Partikeloberfläche IN der Zelle aus?”

Wir werden ausserdem Nanostächen herstellen und untersuchen, ob es chemisch möglich ist, diese Stäbchen nur einseitig zu verändern. Das übergeordnete Ziel ist die Herstellung von multi-Nanopartikel- bzw multi-Nanostäbchen-Strukturen. Diese Strukturen könnte man dann gezielt “bauen” und dementsprechend sehr unterschiedliche Geometrien im biologischen Kontext studieren.

Wissenschaftlicher und gesellschaftlicher Kontext des Forschungspro- jekts

Unsere Arbeit wird wichtige Informationen über Nanopartikel und deren Wechselwirkungen mit biologischen Komponenten generieren. Dies ist einerseits notwendig, um diese Materialien der Nanomedizin zugänglich zu machen. Andererseits werden die erhaltenen Ergebnisse wesentlich dazu beitragen, das Risiko dieser synthetischen kleinen Partikel für den Menschen abzuschätzen.

Direct link to Lay Summary Last update: 28.08.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Plasmonic nanoparticles and their characterization in physiological fluids
Urban D., Rodriguez-Lorenzo L., Balog S., Kinnear C., Rothen-Rutishauser B., Petri-Fink A. (2016), Plasmonic nanoparticles and their characterization in physiological fluids, in Colloids and Surfaces B: Biointerfaces , 137, 39.
Fate of Cellulose Nanocrystal Aerosols Deposited on the Lung Cell Surface In Vitro
Endes C., Mueller S., Kinnear C., Vanhecke D., Foster E., Petri-Fink A., Weder C., Clift M., Rothen- (2015), Fate of Cellulose Nanocrystal Aerosols Deposited on the Lung Cell Surface In Vitro, in Biomacromolecules, 16(4), 1267-1275.
Nanoparticle colloidal stability in cell culture media and impact on cellular interactions
Moore T., Rodriguez-Lorenzo L., Hirsch V., Balog S., Urban D., Jud C., Rothen-Rutishauser B., Lattua (2015), Nanoparticle colloidal stability in cell culture media and impact on cellular interactions, in Chemical Society Reviews, 44(17), 6287.
Ultrathin Ceramic Membranes as Scaffolds for Functional Cell Coculture Models on a Biomimetic Scale
Jud C., Ahmed S., Müller L., Kinnear C., Vanhecke D., Umehara Y., Frey S., Liley M., Angeloni S., Pe (2015), Ultrathin Ceramic Membranes as Scaffolds for Functional Cell Coculture Models on a Biomimetic Scale, in BioResearch Open Access , 4(1), 457.
Fluorescence-Encoded Gold Nanoparticles: Library Design and Modulation of Cellular Uptake into Dendritic Cells
Rodriguez-Lorenzo Laura, Fytianos Kleanthis, Blank Fabian, Von Garnier Christophe, Rothen-Rutishauser Barbara M, Petri-Fink Alke (2014), Fluorescence-Encoded Gold Nanoparticles: Library Design and Modulation of Cellular Uptake into Dendritic Cells, in Small, 10(7), 1440-1440.
In vitro dosimetry of agglomerates
Hirsch V., Kinnear C., Rodriguez-Lorenzo L., Monnier C. A., Rothen-Rutishauser B., Balog S., Petri-Fink A. (2014), In vitro dosimetry of agglomerates, in Nanoscale, 6(13), 7325-7331.
Insertion of Nanoparticle Clusters into Vesicle Bilayers
Bonnaud Cécile, Monnier Christophe A, Demurtas Davide, Jud Corinne, Vanhecke Dimitri, Montet Xavier, Hovius Ruud, Lattuada Marco, Rothen-Rutishauser Barbara M, Petri-Fink Alke (2014), Insertion of Nanoparticle Clusters into Vesicle Bilayers, in {ACS} Nano, 8(4), 3451-3460.
Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization
Digigow Reinaldo G, Dechézelles J.-F., Kaufmann J., Vanhecke D., Knapp H., Lattuada M., Rothen-Rutishauser B., Petri-Fink A. (2014), Magnetic microreactors for efficient and reliable magnetic nanoparticle surface functionalization, in Lab on a Chip, 14(13), 2276-2286.
Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches
Herzog Fabian, Loza Kateryna, Balog Sandor, Clift Martin J D, Epple Matthias, Gehr Peter, Petri-Fink Alke, Rothen-Rutishauser Barbara (2014), Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches, in Beilstein Journal of Nanotechnology, 5, 1357-1370.
Multi-Functional Magnetic Photoluminescent Photocatalytic Polystyrene-Based Micro- and Nano-Fibers Obtained by Electrospinning
Schaer Michel, Crittin Mireille, Kasmi Lamia, Pierzchala Katarzyna, Calderone Caroline, Digigow Reinaldo G, Petri-Fink Alke, Forró László, Sienkiewicz Andrzej (2014), Multi-Functional Magnetic Photoluminescent Photocatalytic Polystyrene-Based Micro- and Nano-Fibers Obtained by Electrospinning, in Fibers 2014, Vol. 2, Pages 75-91, 2(1), 75-91.
Polyvinyl Alcohol as a Biocompatible Alternative for the Passivation of Gold Nanorods
Kinnear Calum, Burnand David, Clift Martin J. D., Kilbinger Andreas F. M., Rothen-Rutishauser Barbara, Petri-Fink Alke (2014), Polyvinyl Alcohol as a Biocompatible Alternative for the Passivation of Gold Nanorods, in Angewandte Chemie International Edition, 12613-12617.
Preparation and characterization of functional silica hybrid magnetic nanoparticles
Digigow Reinaldo G, Dechézelles Jean-François, Dietsch Hervé, Geissbühler Isabelle, Vanhecke Dimitri, Geers Christoph, Hirt Ann M, Rothen-Rutishauser Barbara M, Petri-Fink Alke (2014), Preparation and characterization of functional silica hybrid magnetic nanoparticles, in Journal of Magnetism and Magnetic Materials, 362(0), 72-79.
Gold nanorods: controlling their surface chemistry and complete detoxification by a two-step place exchange.
Kinnear Calum, Dietsch Hervé, Clift Martin J D, Endes Carola, Rothen-Rutishauser Barbara M, Petri-Fink Alke (2013), Gold nanorods: controlling their surface chemistry and complete detoxification by a two-step place exchange., in Angew.Chem.Int.Ed., 52(7), 1934-1938.
Influence of Serum Supplemented Cell Culture Medium on Colloidal Stability of Polymer Coated Iron Oxide and Polystyrene Nanoparticles With Impact on Cell Interactions In Vitro
Hirsch Vera, Salaklang Jatuporn, Rothen-Rutishauser Barbara M, Petri-Fink Alke (2013), Influence of Serum Supplemented Cell Culture Medium on Colloidal Stability of Polymer Coated Iron Oxide and Polystyrene Nanoparticles With Impact on Cell Interactions In Vitro, in IEEE Transactions on Magnetics, 49(1), 402-407.
Nanomaterials and the human lung: What is known and what must be deciphered to realise their potential advantages?
Jud Corinne, Clift Martin J D, Petri-Fink Alke, Rothen-Rutishauser Barbara M (2013), Nanomaterials and the human lung: What is known and what must be deciphered to realise their potential advantages?, in Swiss Medical Weekly, 143, 13758-13758.
Size-Dependent Uptake of Particles by Pulmonary Antigen-Presenting Cell Populations and Trafficking to Regional Lymph Nodes
Blank Fabian, Stumbles Philip A, Seydoux Emilie, Holt Patrick G, Petri-Fink Alke, Rothen-Rutishauser Barbara M, Strickland Deborah H, Von Garnier Christophe (2013), Size-Dependent Uptake of Particles by Pulmonary Antigen-Presenting Cell Populations and Trafficking to Regional Lymph Nodes, in American Journal of Respiratory Cell and Molecular Biology, 49(1), 67-77.
Spatial {SPION} Localization in Liposome Membranes
Bonnaud Cécile, Vanhecke Dimitri, Demurtas Davide, Rothen-Rutishauser Barbara M, Petri-Fink Alke (2013), Spatial {SPION} Localization in Liposome Membranes, in {IEEE} Transactions on Magnetics, 49(1), 166-171.
Surface charge of polymer coated {SPIONs} influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro.
Hirsch Vera, Kinnear Calum, Moniatte Marc, Rothen-Rutishauser Barbara M, Clift Martin J D, Petri-Fink Alke (2013), Surface charge of polymer coated {SPIONs} influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro., in Nanoscale, 5(9), 3723-3732.
Catechol-derivatized poly(vinyl alcohol) as a coating molecule for magnetic nanoclusters
Burnand David, Monnier Christophe A., Redjem Anthony, Schaefer Mark, Rothen-Rutishauser Barbara, Kilbinger Andreas, Petri-Fink Alke, Catechol-derivatized poly(vinyl alcohol) as a coating molecule for magnetic nanoclusters, in Journal of Magnetism and Magnetic Materials.
Nanoparticle Polydispersity Can Strongly Affect In Vitro Dose
Rodriguez-Lorenzo Laura, Rothen-Rutishauser Barbara, Petri-Fink Alke, Balog Sandor, Nanoparticle Polydispersity Can Strongly Affect In Vitro Dose, in Particle & Particle Systems Characterization.
Thermally Reversible Self-Assembly of Nanoparticles via Polymer Crystallization
Kinnear Calum, Balog Sandor, Rothen-Rutishauser Barbara, Petri-Fink Alke, Thermally Reversible Self-Assembly of Nanoparticles via Polymer Crystallization, in Macromolecular Rapid Communications.
Uptake and Intracellular Fate of Peptide Surface-Functionalized Silica Hybrid Magnetic Nanoparticles In Vitro
Digigow Reinaldo G., Vanhecke Dimitri, Rothen-Rutishauser Barbara, Clift Martin J.D., Petri-Fink Alke, Uptake and Intracellular Fate of Peptide Surface-Functionalized Silica Hybrid Magnetic Nanoparticles In Vitro, in Particle & Particle Systems Characterization.

Collaboration

Group / person Country
Types of collaboration
University Bern Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
EPFL Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
University Vigo Spain (Europe)
- Publication
- Research Infrastructure
- Exchange of personnel
University Lund Sweden (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
European Commission Joint Research Centre Nanobiotechnology Workshop Talk given at a conference Gold Nanoparticle Characterization in Complex Biological Media: Implications for understanding nanoparticle behavior in biomedical applications 24.11.2015 Ispra, Italy Moore Thomas Lee;
PDA Europe Conference: Particles in Injectables Talk given at a conference Considerations for Measuring In Vitro Toxicity of Nanomaterials 10.09.2015 Berlin, Germany Moore Thomas Lee;
Clinam 2015 Talk given at a conference Things you should be aware of when working with nanoparticles and cells 29.06.2015 Basel, Switzerland Petri-Fink Alke;
ImagineNano 2015, Talk given at a conference Designing smart vesicles for drug delivery 12.03.2015 Bilbao, Spain Petri-Fink Alke;
NanoBio Australia Talk given at a conference The impact of nanoparticle shape on cell interaction 07.07.2014 Brisbane, Australia Kinnear Calum;
CLINAM Talk given at a conference Janus Magnetic Liposomes 23.06.2014 Basel, Switzerland Petri-Fink Alke;
Swiss Nanoconvention Talk given at a conference When "Nanomaterials meet Cells” 21.05.2014 Brugg, Switzerland Petri-Fink Alke;
ACS International Meeting Talk given at a conference Stimuli-responsive nanomaterials for drug delivery 16.03.2014 Dallas, United States of America Petri-Fink Alke;


Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Formation continue 26.11.2014 Fribourg, Switzerland Petri-Fink Alke;


Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions Expo Nano Podium Western Switzerland German-speaking Switzerland 2015
Media relations: radio, television Drug delivery Radio FR Western Switzerland 2014
Talks/events/exhibitions Smart nanomaterials Radio televisione Svizzera German-speaking Switzerland Western Switzerland 2014

Associated projects

Number Title Start Funding scheme
123373 Advances in Nanoparticle Engineering with a focus on Stability, Surface, and Particle-Cell Interaction 01.10.2009 SNSF Professorships

Abstract

The here proposed project is the logic continuation of the original project entitled “Advances in Nanoparticle Engineering with a focus on Stability, Surface, and Particle-Cell Interaction”. This follow-up project is again situated at the interface between nanomaterials science and nanobiology and shall investigate three research questions, which are of uttermost importance in the field. As such, the project will investigate 1) how nanoparticle-protein interactions change when nanoparticles are internalized into cells (Will the protein corona be preserved after nanoparticle uptake by the cell?).2) the extent and geometry of anisotropic nanoparticle surface functionalization and the impact on cellular interaction.
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