Blue light-activated disinfection; Endodontic infection; Metagenomics
(2018), Enzyme-mediated photoinactivation of Enterococcus faecalis using Rose Bengal-acetate., in
J Photochem Photobiol B, 84-90.
Manoil Daniel, Filieri Anna, Schrenzel Jacques, Bouillaguet Serge, Manoil Daniel, Filieri Anna, Schrenzel Jacques, Bouillaguet Serge (2016), Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry., in
Journal of photochemistry and photobiology. B, Biology, 162, 258-65.
Marinic Karlo, Manoil Daniel, Filieri Anna, Wataha John C, Schrenzel Jacques, Lange Norbert, Bouillaguet Serge, Marinic Karlo, Manoil Daniel, Filieri Anna, Wataha John C, Schrenzel Jacques, Lange Norbert, Bouillaguet Serge (2015), Repeated exposures to blue light-activated eosin Y enhance inactivation of E. faecalis biofilms, in vitro., in
Photodiagnosis and photodynamic therapy, 12(3), 393-400.
Bulit Florence, Grad Iwona, Manoil Daniel, Simon Stéphane, Wataha John C, Filieri Anna, Feki Anis, Schrenzel Jacques, Lange Norbert, Bouillaguet Serge, Bulit Florence, Grad Iwona, Manoil Daniel, Simon Stéphane, Wataha John C, Filieri Anna, Feki Anis, Schrenzel Jacques, Lange Norbert, Bouillaguet Serge (2014), Antimicrobial activity and cytotoxicity of 3 photosensitizers activated with blue light., in
Journal of endodontics, 40(3), 427-31.
Manoil Daniel, Filieri Anna, Gameiro Cécile, Lange Norbert, Schrenzel Jacques, Wataha John C, Bouillaguet Serge, Manoil Daniel, Filieri Anna, Gameiro Cécile, Lange Norbert, Schrenzel Jacques, Wataha John C, Bouillaguet Serge (2014), Flow cytometric assessment of Streptococcus mutans viability after exposure to blue light-activated curcumin., in
Photodiagnosis and photodynamic therapy, 11(3), 372-9.
In our previous research project (# 310000 11993) we generated the following data that suggest that blue light -mediated PAD is an appealing option for endodontic disinfection. First, we have identified several photosensitizers which generate ROS upon blue light irradiation and have established the in vitro efficacy of these photosensitizers as antimicrobial agents against major Gram-positive endodontic pathogens. Second, we have verified that these photo-activated chemicals do not elicit unwanted toxicity towards resident cells at bactericidal concentrations, thus indicating a favorable therapeutic index. Although these key experiments have demonstrated that blue light-activated disinfection has the potential to induce lethal oxidative stresses in bacteria with minimum adverse effects to host cells, several limitations have also been identified. Among these were a poor efficiency against some Gram-negative pathogens and a reduced efficiency on bacterial biofilms compared to planktonic suspensions. Further, most recent research using culture-independent methods has severely questioned the relevance of the endodontic microbiota previously identified by culture-based methods. Therefore, in the current proposal, we will:•Develop photosensitizer-conjugates required for enhanced ROS production and improved efficacy against gram negative endodontic pathogens •Improve our understanding of antimicrobial efficacy using 3D biofilm models and validate our in vitro results using metagenomic approaches on clinical samples We are hopeful that the combination of the unique electromagnetic properties of blue light with optimized photosensitizers will synergize to make blue light-mediated PAD a successful treatment for endodontic infection. Furthermore, the unique structure of this project that combines, dentistry, chemistry and microbiology will provide us with new knowledge on endodontic infections and antibacterial treatments.