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Elucidating the mechanisms underlying volatile-mediated growth inhibition of Phytophthora infestans by rhizosphere bacteria

English title Elucidating the mechanisms underlying volatile-mediated growth inhibition of Phytophthora infestans by rhizosphere bacteria
Applicant Weisskopf Laure
Number 149271
Funding scheme Project funding
Research institution Haute école de viticulture et oenologie Haute Ecole Spécialisée de Suisse Occidental
Institution of higher education Research Institutes Agroscope - AGS
Main discipline Experimental Microbiology
Start/End 01.03.2014 - 28.02.2018
Approved amount 392'000.00
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All Disciplines (3)

Discipline
Experimental Microbiology
Botany
Molecular Biology

Keywords (6)

Pseudomonas; Phytophthora; Volatile; PGPR; Biocontrol; Microbial ecology

Lay Summary (French)

Lead
Les bactéries produisent des composés volatiles qui jouent un rôle important dans les interactions avec d’autres organismes. La structure chimique de ces molécules, ainsi que le mode d’action dans les organismes cibles, sont encore largement inconnus à ce jour. Le but de ce projet est de contribuer à identifier les molécules actives contre l’agent du mildiou de la pomme de terre et de comprendre les mécanismes qui conduisent à l’inhibition du développement de ce pathogène.
Lay summary

Les bactéries produisent des composés volatiles dont l’importance dans les interactions avec d’autres organismes a été largement démontrée aux cours des dernières années. Ces composés volatiles sont notamment capables de promouvoir la croissance des plantes ou de réduire celle des champignons phytopathogènes. La structure chimique de ces molécules volatiles actives est pour la plupart encore inconnue, de même que leur mode d’action dans les plantes ou les champignons pathogènes. Le but de ce projet est d’identifier les molécules actives et d’explorer les mécanismes par lesquels des bactéries naturellement associées aux pommes de terre promeuvent la croissance de cette plante et inhibent le développement de Phytophthora infestans,  l’agent du mildiou de la pomme-de-terre, qui constitue la maladie principale de cette culture.  

Ce projet de recherche fondamentale est orienté vers l’application. Le but à long terme est d’exploiter au mieux le potentiel microbiologique des sols et plus particulièrement l’effet bénéfique de souches bactériennes naturellement associées aux pommes de terre. Cette recherche s’inscrit dans une volonté de trouver des alternatives aux traitements chimiques et de contribuer ainsi à l’intensification écologique de l’agriculture.

 


Direct link to Lay Summary Last update: 13.02.2014

Responsible applicant and co-applicants

Employees

Publications

Publication
Linking comparative genomics of nine potato-associated Pseudomonas isolates with their differing biocontrol potential against late blight
De Vrieze Mout, Varadarajan Adithi R., Schneeberger K., Bailly Aurélien, Ahrens C.H., Weisskopf Laure (2020), Linking comparative genomics of nine potato-associated Pseudomonas isolates with their differing biocontrol potential against late blight, in Frontiers in Microbiology, 11(April), 857-857.
A sulfur-containing volatile emitted by potato-associated bacteria confers protection against late blight through direct anti-oomycete activity
Chinchilla Delphine, Bruisson Sébastien, Meyer Silvan, Zühlke Daniela, Hirschfeld Claudia, Joller Charlotte, L'Haridon Floriane, Mène-Saffrané Laurent, Riedel Katharina, Weisskopf Laure (2019), A sulfur-containing volatile emitted by potato-associated bacteria confers protection against late blight through direct anti-oomycete activity, in Scientific Reports, 18778.
Biocontrol activity of three Pseudomonas on a newly assembled collection of Phytophthora infestans isolates
De Vrieze Mout, Gloor Ramona, Massana Codina Josep, Torriani Stefano, Gindro Katia, L'Haridon Floriane, Bailly Aurélien, Weisskopf Laure (2019), Biocontrol activity of three Pseudomonas on a newly assembled collection of Phytophthora infestans isolates, in Phytopathology, 109, 1555-1565.
Combining Different Potato-Associated Pseudomonas Strains for Improved Biocontrol of Phytophthora infestans
De Vrieze Mout, Germanier Fanny, Vuille Nicolas, Weisskopf Laure (2018), Combining Different Potato-Associated Pseudomonas Strains for Improved Biocontrol of Phytophthora infestans, in Frontiers in Microbiology, 9(October), 1-13.
Microbial life in the grapevine: what can we expect from the leaf microbiome?
Vionnet Léo, De Vrieze Mout, Dutartre Agnès, Gfeller Aurélie, Lüthi Angelika, L'Haridon Floriane, Weisskopf Laure (2018), Microbial life in the grapevine: what can we expect from the leaf microbiome?, in OENO One, 52(3), 205-210.
Mining the Volatilomes of Plant-Associated Microbiota for New Biocontrol Solutions
Bailly Aurélien, Weisskopf Laure (2017), Mining the Volatilomes of Plant-Associated Microbiota for New Biocontrol Solutions, in Frontiers in Microbiology, 8, 1638.
Smelly Fumes: Volatile-Mediated Communication between Bacteria and Other Organisms
Laure Weisskopf, Choong-Min Ryu, Jos M. Raaijmakers, Paolina Garbeva (2017), Smelly Fumes: Volatile-Mediated Communication between Bacteria and Other Organisms, Frontiers Media SA, Switzerland.
Long-Chain Alkyl Cyanides: Unprecedented Volatile Compounds Released by Pseudomonas and Micromonospora Bacteria.
Montes Vidal Diogo, von Rymon-Lipinski Anna-Lena, Ravella Srinivasa, Groenhagen Ulrike, Herrmann Jennifer, Zaburannyi Nestor, Zarbin Paulo H G, Varadarajan Adithi R, Ahrens Christian H, Weisskopf Laure, Müller Rolf, Schulz Stefan (2017), Long-Chain Alkyl Cyanides: Unprecedented Volatile Compounds Released by Pseudomonas and Micromonospora Bacteria., in Angewandte Chemie (International ed. in English), 56(15), 4342-4346.
Editorial: Smelly Fumes: Volatile-Mediated Communication between Bacteria and Other Organisms.
Weisskopf Laure, Ryu Choong-Min, Raaijmakers Jos M, Garbeva Paolina (2016), Editorial: Smelly Fumes: Volatile-Mediated Communication between Bacteria and Other Organisms., in Frontiers in microbiology, 7, 2031-2031.
The Anti-Phytophthora Effect of Selected Potato-Associated Pseudomonas Strains: From the Laboratory to the Field
Guyer Anouk, De Vrieze Mout, Bönisch Denise, Gloor Ramona, Musa Tomke, Bodenhausen Natacha, Bailly Aurélien, Weisskopf Laure (2015), The Anti-Phytophthora Effect of Selected Potato-Associated Pseudomonas Strains: From the Laboratory to the Field, in Frontiers in Microbiology, 6, 1.
Pseudomonas strains naturally associated with potato plants produce volatiles with high potential for inhibition of Phytophthora infestans
Hunziker Lukas, Bönisch Denise, Groenhagen Ulrike, Bailly Aurélien, Schulz Stefan, Weisskopf Laure (2015), Pseudomonas strains naturally associated with potato plants produce volatiles with high potential for inhibition of Phytophthora infestans, in Applied and Environmental Microbiology, 81(3), 821-830.
Volatile organic compounds from native potato-associated Pseudomonas as potential anti-oomycete agents
De Vrieze Mout, Pandey Piyush, Bucheli Thomas D., Varadarajan Adithi R., Ahrens Christian H., Weisskopf Laure, Bailly Aurélien (2015), Volatile organic compounds from native potato-associated Pseudomonas as potential anti-oomycete agents, in Frontiers in Microbiology, 6(NOV), 1-15.
The inter-kingdom volatile signal indole promotes root development by interfering with auxin signalling
Bailly Aurelien, Groenhagen Ulrike, Schulz Stefan, Geisler Markus, Eberl Leo, Weisskopf Laure (2014), The inter-kingdom volatile signal indole promotes root development by interfering with auxin signalling, in PLANT JOURNAL, 80(5), 758-771.

Collaboration

Group / person Country
Types of collaboration
Dr. Katia Gindro, Agroscope Changins Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Dr. Christian Ahrens, Agroscope Wädenswil Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Kathrin Riedel, Institute of Microbiology, University of Greifswald Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Dr. Paolina Garbeva, NIOO Netherlands (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Stefan Schulz, Institute for Organic Chemistry, TU Braunschweig Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Dr. Thomas Bucheli, Agroscope Reckenholz Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Pascal Fuchsmann, Agroscope Liebefeld Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
Swiss Plant Science Meeting Poster Exploring the action mechanisms of anti-microbial volatile organic compounds emitted by potato-associated Pseudomonas 01.02.2018 Meiringen, Switzerland Chinchilla Delphine;
Swiss Plant Science Meeting Individual talk News from the volatile warfare between Pseudomonas and Phytophthora infestans 01.02.2018 Meiringen, Switzerland Weisskopf Laure;
MiCROPe Talk given at a conference News from the volatile warfare between potato-associated Pseudomonas and the late blight causing agent Phytophthora infestans 04.12.2017 Schloss Schönbrunn, Vienna, Austria Weisskopf Laure;
Invited seminar Individual talk The smell of bacteria and its impact on plant growth and health 27.11.2017 University of Bern, Switzerland Weisskopf Laure;
Invited seminar Individual talk The smell of bacteria and its impact on plant growth and health 06.11.2017 University of Geneva, Switzerland Weisskopf Laure;
Minisymposium "interactions matter" Talk given at a conference Volatile sulfur compounds from bacteria as potent anti-oomycete agents 20.10.2017 Netherland's Institute of Ecology, Wageningen, Switzerland Weisskopf Laure;
Plant Bioprotech Talk given at a conference The potential of bacterial volatiles for plant protection against diseases 28.06.2017 University of Reims, France Weisskopf Laure;
Oenoviti 2017 Talk given at a conference What can we expect from the leaf microbiome? 16.05.2017 HES-SO Changins, Nyon, Switzerland Weisskopf Laure;
Mycology Symposium Talk given at a conference The potential of bacterial volatiles for plant protection against diseases 27.01.2017 ETH Zurich, Switzerland Weisskopf Laure;
Annual IOBC (international organization of biological control) meeting Talk given at a conference Sulfur volatiles: new weapons in the fight against plant diseases 15.09.2016 University of Berlin, Germany Weisskopf Laure;
Ecosummit Meeting Talk given at a conference Bacterial volatiles and their impact on plant growth and health 30.08.2016 Montpellier, France Gfeller Aurélie; Bailly Aurélien; Weisskopf Laure;
Macrowine 2016 Talk given at a conference What can we expect from the leaf microbiome? A focus on its smell 29.06.2016 HES-SO Changins, Nyon, Switzerland Weisskopf Laure;
Invited seminar Individual talk A smelly world – bacterial volatiles and their impact on plant growth and health 26.05.2016 University of Neuchâtel, Switzerland Weisskopf Laure;
Invited seminar Individual talk Bacteria-fungi interactions in the soil, the rhizosphere and the phyllosphere 28.01.2016 University of Greifswald, Germany Weisskopf Laure;
Symposium on plant-microbe interactions Individual talk Les odeurs bactériennes et leur impact sur la croissance et la santé des plantes 20.11.2015 University of Fribourg, Switzerland Weisskopf Laure;
Rhizosphere conference Poster The potato microbiome and its potential impact on late blight resistance 21.06.2015 Maastricht, Netherlands Bailly Aurélien;
PGPR meeting Talk given at a conference Using bacterial volatiles to fight Phytophthora infestans: new solution to an old problem? 17.06.2015 University of Liège, Belgium Weisskopf Laure;
Rhizosphere conference Poster When signaling gets smelly: bacterial volatiles and their effects on rhizosphere inhabitants 01.06.2015 Maastricht, Netherlands Weisskopf Laure;
Invited seminar Individual talk A smelly tale of bacteria, plants and fungi 22.04.2015 ETH Zürich, Switzerland Weisskopf Laure;
2. Agroscope Nachhaltigkeitstagung Talk given at a conference Das Pflanzenmikrobiom und seine schützende Wirkung gegen Krankheitserreger 22.01.2015 Reckhenholz Zürich, Switzerland Weisskopf Laure;
Invited seminar Individual talk A smelly tale of bacteria, plants and fungi 15.12.2014 Helmoltz Zentrum München, Germany Weisskopf Laure;
Invited seminar Individual talk A smelly tale of bacteria, plants and fungi 11.12.2014 University of Basel, Switzerland Weisskopf Laure;
Annual meeting of the Swiss Society for Phytopathology (SGP) Poster The potential of rhizosphere and phyllosphere Pseudomonads for late blight control 25.09.2014 Wädenswil, Switzerland Bailly Aurélien; Weisskopf Laure;
Annual meeting of the Swiss Society for Phytopathology (SGP) Poster Toxic waste or chemical warfare? Bacterial airborne threat thwarts fungi's scorched earth policy 25.09.2014 Wädenswil, Switzerland Bailly Aurélien;
Annual IOBC (international organization of biological control) meeting Talk given at a conference The potential of potato-associated Pseudomonas strains for biological control of Phytophthora infestans 16.06.2014 Upsalla, Sweden Weisskopf Laure; Bailly Aurélien;
Invited seminar Individual talk Bacterial volatiles and their effects on plants and phytopathogenic fungi 14.04.2014 Netherland's Institute of Ecology, Wageningen, Netherlands Weisskopf Laure;


Knowledge transfer events

Active participation

Title Type of contribution Date Place Persons involved
Plant protection day (meeting with cantonal delegates for crop protection) Talk 02.02.2015 Morat, Switzerland Weisskopf Laure;
Workshop for organic potato growers in Switzerland Talk 10.12.2014 Grangeneuve (agricultural school of Fribourg), Switzerland Weisskopf Laure;
Visit of the potato grower association (Swisspatat); presentation of ongoing experiments Workshop 01.07.2014 Zurich Reckenholz, Switzerland Weisskopf Laure; Bailly Aurélien;


Communication with the public

Communication Title Media Place Year
Media relations: print media, online media Bakterien gegen Krautfäule Top Agrar German-speaking Switzerland International 2015
Media relations: print media, online media Das Potenzial von Phosphonaten und Pflanzenextrakten Kartoffelbau German-speaking Switzerland International 2015
Media relations: print media, online media Bakterien aus dem Wurzelbereich hemmen den Erreger der Kraut- und Knollenfäule Agrarforschung Schweiz Western Switzerland Italian-speaking Switzerland German-speaking Switzerland 2014
Media relations: print media, online media Nützliche Bakterien / Des bactéries à la rescousse UFA Revue Western Switzerland German-speaking Switzerland 2014

Associated projects

Number Title Start Funding scheme
130089 Dominance of Burkholderia sp. in low pH Environments: from Biogeography to Tolerance Mechanisms 01.09.2010 Project funding
179310 Volatile-mediated communication between bacteria, plants, and plant pathogens 01.05.2018 Project funding
175388 The role of volatile organic compounds emitted by Paenibacillus alvei in the biological control of the plant pathogenic fungus Verticillium dahliae 01.07.2017 International short research visits

Abstract

During the last decade, the importance of bacterial volatiles in cross-kingdom interactions has become evident. In addition to promoting plant growth and root development, bacterial volatiles have been repeatedly shown to inhibit the growth of phytopathogenic fungi, although the molecules responsible for this effect are still largely unknown. Likewise, the mechanisms underlying the observed growth inhibition have never yet been investigated. In this proposal, we aim at elucidating the mechanisms by which Pseudomonas strains newly isolated from infested potato plants are able to induce complete growth arrest in the late blight causing agent Phytophthora infestans.
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