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Transporters for di- and tripeptides in Arabidopsis

English title Transporters for di- and tripeptides in Arabidopsis
Applicant Rentsch Doris
Number 149229
Funding scheme Project funding
Research institution Institut für Pflanzenwissenschaften Universität Bern
Institution of higher education University of Berne - BE
Main discipline Botany
Start/End 01.01.2014 - 31.07.2018
Approved amount 439'000.00
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All Disciplines (3)

Discipline
Botany
Cellular Biology, Cytology
Molecular Biology

Keywords (8)

accession; Arabidopsis thaliana; plasma membrane; tonoplast; mutant; transport; organic nitrogen; peptide

Lay Summary (German)

Lead
Stickstoff ist für Wachstum und Ertrag von Pflanzen essentiell und wird Kulturpflanzen in der Regel in Form von Dünger zugeführt. Die ineffiziente Nutzung von anorganischem Stickstoff kann zu dessen Auswaschung und damit unter anderem zu Beeinträchtigungen anderer Ökosysteme führen. Die Pflanze kann auch organische Stickstoffverbindungen nutzen. Inwieweit letztere zur Pflanzenernährung beitragen ist jedoch immer noch wenig erforscht und Gegenstand wissenschaftlicher Diskussionen.
Lay summary

Unsere Forschung hat zum Ziel die Aufnahme und Verteilung von organischem Stickstoff in Pflanzen besser zu verstehen. Insbesondere interessiert uns die Aufnahme und der Metabolismus kleiner Peptide. Im Projekt versuchen wir Arabidopsis-Mutanten zu identifizieren, die ein verbessertes oder eingeschränktes Wachstum auf Peptiden als Stickstoffquelle aufweisen, um Transporter, Regulatoren und Enzyme zu identifizieren, die an der effizienten Nutzung von organischem Stickstoff beteiligt sind. Neben diesem generellen Ansatz untersuchen wir andererseits den Beitrag von Peptidtransportern für die Stickstoffversorgung der Pflanzen und charakterisieren die beteiligten Transportproteine.

 

Detaillierte Kenntnisse zur Aufnahme und Verteilung von organischem Stickstoff und dessen Transport in Ernteprodukte wie beispielsweise Samen tragen zur Klärung der Rolle von organischem Stickstoff für die Produktivität der Pflanzen bei. Erkenntnisse aus diesen Arbeiten können möglicherweise in der Pflanzenzüchtung und für eine nachhaltige Landwirtschaft genutzt werden.

 

Direct link to Lay Summary Last update: 20.12.2013

Lay Summary (English)

Lead
Nitrogen (N) is limiting plant growth and reproduction. While inorganic N represents the largest pool of soluble N in many fertilized soils, organic N forms, including amino acids, peptides and proteins, often dominate non-fertilized soils. There is increasing evidence that in addition to inorganic N, plants also acquire organic N. It remains however unclear, how much the direct uptake of organic N contributes to plant N needs.
Lay summary

Aim of the project

Our research has contributed to the current understanding of the uptake and reallocation of amino acids and peptides in plants. In our current work, we aim to deepen this research by exploring peptide transporter function and regulation, as well as peptide metabolism.

The interest in organic N as plant N sources is driven by a need for sustainable agricultural practices. Inorganic N dominated, fertilized soils lose N and cause pollution of the environment, as well as a carrying a large energy footprint of synthetic N fertilizer manufactures. Increased understanding on organic N uptake and distribution for plant productivity and growth, may contribute to improve future plant breeding and sustainable agriculture.

 

Direct link to Lay Summary Last update: 20.12.2013

Responsible applicant and co-applicants

Employees

Collaboration

Group / person Country
Types of collaboration
Prof. Thomas E. Junger, University of Texas, Austin United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Exchange of personnel
Prof. Christine Ziegler, MPI Biophysics Frankfurt/University of Regensburg, Frankfurt/Regensburg Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Exchange of personnel
Prof. Susanne Schmidt, University of Queensland, Brisbane Australia (Oceania)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Gerd Jürgens Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Enrico Martinoia, University of Zürich, Zürich Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Prof. Jürgen Denecke, University of Leeds Great Britain and Northern Ireland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Prof. Karin Schumacher Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
SwissPlant 2018 Poster Identifying regulators of di- and tripeptide utilization in Arabidopsis 31.01.2018 Meiringen, Switzerland Freihart Michaela; Rentsch Doris; Arens Kirsten;
Kolloquium Individual talk Uptake and reallocation of organic N – The role of dipeptide transporter 18.05.2017 Beijing, China Rentsch Doris;
International Workshop: Nutrient stewardship & next-generation fertilisers Talk given at a conference Uptake and reallocation of organic N – The role of dipeptide transporters 09.12.2016 Heron Island, Australia Rentsch Doris;
EMBO conference ‐ The nitrogen nutrition of plants. Nitrogen 2016: Third International Symposium on the Nitrogen Nutrition of Plants Talk given at a conference Uptake and reallocation of organic N – The role of dipeptide transporters 22.08.2016 Montpellier, France Rentsch Doris;
Gordon research conference - Molecular Plant Biology Poster Identifying regulators of di- and tripeptide utilization in Arabidopsis 12.06.2016 Hoderness, United States of America Rentsch Doris; Freihart Michaela; Arens Kirsten;
Gordon research seminar - Molecular Plant Biology Poster Identifying regulators of di- and tripeptide utilization in Arabidopsis 11.06.2016 Holderness, United States of America Rentsch Doris; Arens Kirsten; Freihart Michaela;
Kolloquium Individual talk Uptake and reallocation of organic N – The role of dipeptide transporters 10.06.2016 Toronto, Canada Rentsch Doris;
17th International Workshop on Plant Membrane Biology Annapolis Poster Identifying regulators of di- and tripeptide utilization in Arabidopsis thaliana 05.06.2016 Annapolis, United States of America Rentsch Doris; Arens Kirsten; Freihart Michaela;
SwissPlant 2016 Poster Identifying regulators of di- and tripeptide utilization in Arabidopsis thaliana 25.01.2016 Les Diablerets, Switzerland Rentsch Doris; Arens Kirsten; Freihart Michaela;
Kolloquium Individual talk Peptide transporters in Arabidopsis: Role in intra- and intercellular transport of organic nitrogen 23.11.2015 Haifa, Israel Rentsch Doris;
CUSO MPS PhD retreat Poster Identifying regulators of di- and tripeptide utilization in Arabidopsis thaliana 08.06.2015 Münchenwiler, Switzerland Rentsch Doris; Freihart Michaela; Arens Kirsten;
Kolloquium Individual talk Transport of organic nitrogen in plants 26.10.2014 Konstanz, Germany Rentsch Doris;
TNAM 2014. 9th Tri National Arabidopsis Meeting Poster Intracellular sorting and trafficking of membrane proteins to the tonoplast 24.09.2014 Heidelberg, Germany Rentsch Doris;
FOR 1061: 2nd International Meeting Plant Primary Metabolism: Synthesis, Storage and Degradation Processes Talk given at a conference Peptide transporters in Arabidopsis: Role in intra- and intercellular transport of organic nitrogen 07.05.2014 Heidelberg, Germany Rentsch Doris;
First international workshop on organic nitrogen and plant nutrition – from molecular mechanisms to ecosystems Poster Determinants responsible for substrate selectivity in the PTR/NRT1 family of Arabidopsis. OrgN2014 09.02.2014 Monte Verità, Switzerland Gerna Davide; Rentsch Doris;
OrgN2014. First international workshop on organic nitrogen and plant nutrition – from molecular mechanisms to ecosystems Talk given at a conference Physiological role and regulation of di- and tripeptide transporters in Arabidopsis 09.02.2014 Monte Verità, Switzerland Rentsch Doris;
OrgN2014. First international workshop on organic nitrogen and plant nutrition – from molecular mechanisms to ecosystem Poster Regulators of peptide transport and metabolism in Arabidopsis thaliana 09.02.2014 Monte Verità, Switzerland Freihart Michaela; Rentsch Doris;
Swissplant 2014 Poster Regulators of peptide transport and metabolism in Arabidopsis thaliana 29.01.2014 Meiringen, Switzerland Rentsch Doris; Freihart Michaela;


Self-organised

Title Date Place
OrgN2014. First international workshop on organic nitrogen and plant nutrition – from molecular mechanisms to ecosystems 09.02.2014 Monte Verità, Switzerland

Associated projects

Number Title Start Funding scheme
157884 Supercritical fluid chromatography tandem mass spectrometry as a new analytical tool for plant sciences 01.06.2015 R'EQUIP
141913 Transporters of Trypanosoma brucei: Phylogeny - Physiology - Pharmacology 01.06.2013 Sinergia
127340 Transporters for di- and tripeptides in Arabidopsis 01.04.2010 Project funding
182426 Amino acid transport, regulation and sensing in Trypanosoma brucei 01.09.2019 Project funding
107507 Transporters for di- and tripeptides in Arabidopsis 01.10.2005 Project funding
163502 Simultaneous manipulation of source and sink metabolism for improved crop yield 01.11.2015 ERA-CAPS

Abstract

Nitrogen (N) is limiting plant growth and reproduction. N uptake from the soil, its assimilation and subsequent reallocation are therefore key processes driving primary productivity of plants. While inorganic N (nitrate and ammonium) represents the largest pool of soluble N in many fertilized soils, organic N forms, including amino acids, peptides and proteins (soluble and bound to the soil matrix), often dominate non-fertilized soils. It is generally assumed that plants use predominantly inorganic N, but there is increasing evidence that plants also acquire organic N from the soil. It remains however unclear, how much the direct uptake of organic N contributes to plant N needs. The interest in organic N as plant N sources is driven by a need for sustainable agricultural practices. Inorganic N dominated, fertilized soils lose N and cause pollution of hydrosphere, atmosphere and pedosphere, as well as a carrying a large energy footprint of synthetic N fertilizer manufactures.Our research has contributed considerably to the current understanding of the uptake and reallocation of peptides in plants. We were first to determine the selectivity and affinities of di/tripeptide transporters, their expression and intracellular localization, and have identified structural determinants required for their delivery to the tonoplast or plasma membrane. We recently isolated, in a low-scale forward genetic screen, several mutants with reduced growth on dipeptides as N source, and identified Arabidopsis accessions with altered performance on organic N.Here, we aim to deepen this research by exploring the trafficking pathways of peptide transporters to their destination membranes and by identifying determinants (i.e. amino acids or domains) important for substrate recognition. We will perform a large-scale genetic screen to search for new mutants, and use already isolated mutants and Arabidopsis accessions to identify and characterize novel genes involved in peptide use. The project will generate (i) fundamental information on the function of peptide transporters, and (ii) provide knowledge on regulation and organic N use in an ecological context. The objectives of the proposed project are to:(i)identify trafficking pathways of peptide transporters to their target membrane(ii)characterize determinants required for substrate selectivity (iii)isolate mutants in a genetic screen with peptides as N sources and characterize mutants to identify novel loci important for function or regulation of peptide metabolism and transport. (iv) explore the of natural genetic variation of Arabidopsis for performance on organic N Our group is very well positioned to perform this research with all required knowledge and materials available for rapid advancement. Heterologous expression systems (yeast and oocytes) for analysis of substrate selectivity, and localization of GFP/fusion proteins for exploring the trafficking pathways, are well established. We will hit the ground running with several mutants and accessions with altered growth on peptides as N source that we have already identified. Knowledge generated in the proposed research will provide a deeper understanding of peptide transporter function and regulation and will advance information on membrane protein trafficking and organic N use. Increased understanding on organic N uptake and distribution for plant productivity and growth, may contribute to improve future plant breeding and sustainable agriculture.
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