Development; Cytoplasmic properties; Cell growth; Microrobotics; Magnetic Manipulation; Nanowires; Nanoprobes; Nanotechnology; Cell biomechanics; Intracellular transport
Hu C. Vogler H. Aellen M. Shamsudhin N. Jang B. Burri J.T. Grossniklaus U. and Nelson (2017), High precision, localized proton gradients and fluxes generated by a microelectrode device induce differential growth behaviors of pollen tubes., in Lab on a Chip
, 17(4), 671-680.
Ahmed D. Baasch T. Jang B. Pane S. Dual J. and Nelson B.J. (2016), Artificial swimmers propelled by acoustically activated flagella, in Nano Letters
, 16(8), 4968-4974.
Jang B. Wang W. Wiget S. Petruska A.J. Chen X. Hu C. Hong A. Folio D. Ferreira A. (2016), Catalytic Locomotion of Core–Shell Nanowire Motors, in ACS nano
, 10(11), 9983-9991.
Sevim S. Ozer S. Feng L. Wurzel J. Fakhraee A. Shamsudhin N. Jang B. Alcantara C. (2016), Dually actuated atomic force microscope with miniaturized magnetic bead-actuators for single-molecule force measurements, in Nanoscale Horizons
, 1(6), 488-495.
Shamsudhin N. Tao Y. Sort J. Jang B. Degen C.L. Nelson B.J. and Pané S. (2016), Magnetometry of Individual Polycrystalline Ferromagnetic Nanowires, in Small
, 12(46), 6363-6369.
Jang B. Chen X.Z. Siegfried R. Moreno J.M.M. Özkale B. Nielsch K. Nelson B.J. and Pané (2015), Silicon-supported aluminum oxide membranes with ultrahigh aspect ratio nanopores, in RSC Advances
, 5(114), 94283-94289.
Jang B. Gutman E. Stucki N. Seitz B.F. Wendel-García P.D. Newton T. Pokki J. Ergenem (2015), Undulatory locomotion of magnetic multilink nanoswimmers., in Nano Letters
, 15(7), 4829-4833.
Jang B. Pellicer E. Guerrero M. Chen X. Choi H. Nelson B.J. Sort J. and Pane S. (2014), Fabrication of segmented Au/Co/Au nanowires: insights in the quality of Co/Au Junctions, in ACS Applied Materials & Interfaces
, 6(16), 14583-14589.
Cellular processes such as morphogenesis, mechano-transduction motility, or cellular movement and migration (e.g., during gastrulation or metastasis), are closely interrelated with the mechanical properties of living cells. While the extracellular environment and biochemical properties play an important role in these processes, it has become clear that environmental physical forces at adhesion sites and contact surfaces also stimulate biochemical processes in cells. Furthermore, internal physical properties of the cytoplasm, largely unexplored, play a central role in morphogenesis and cellular behavior. Understanding the mechanical properties of cells and their response to mechanical stimuli can provide invaluable information regarding cellular processes and culminate in models to describe and predict further cellular responses. This project focuses on developing a new engineering approach for measuring and manipulating the mechanical properties, such as elasticity and viscosity, of cell membranes and their intracellular media, i.e. the cytoplasm and organelles. The wireless manipulation of appropriately designed magnetic nanoprobes along three translational axes, and an additional two or three rotational axes, will enable new types of measurements of mechanical properties at both extra- and intracellular levels. The miniaturized probes will be precisely and wirelessly controlled by a microrobotic system consisting of a multiple-degree-of-freedom electromagnetic platform combined with a fluorescense microscope for the visual tracking of the probes. Furthermore, we will explore approaches to functionalize the nanoprobes, such that they can be used as precise instruments to manipulate internal structures of a cell. A central aspect of this interdisciplinary project is the application of this technology to measure and manipulate internal properties of living cells, for instance to probe the viscoelastic properties of the cytoplasm or to monitor cellular responses to manipulating internal structures, such as the cytoskeleton. In particular, we will focus on measuring viscoelastic properties that exist (i) in the cells of the plant vascular system and (ii) in growing pollen tubes, which are cells with a clear polar axis and unidirectional growth. This interdisciplinary project aims at the development of novel approaches that allow the use of magnetic nanoprobes in living cells, eventually leading to the formulation of models that describe the mechanical properties and behaviors of cells and tissues. Achieving these goals is only possible in the frame of an interdisciplinary team consisting of biologists and engineers, who work closely together to bridge these disciplines, developing and adapting novel tools to address specific biological questions that are related to the intracellular properties of growing cells and tissues.