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Acquisition of a 2-Photon microscope for intravital analysis

English title Acquisition of a 2-Photon microscope for intravital analysis
Applicant Fernandez Gonzalez Santiago
Number 145038
Funding scheme R'EQUIP
Research institution Istituto di ricerca in biomedicina (IRB) Facoltà di scienze biomedice
Institution of higher education Università della Svizzera italiana - USI
Main discipline Immunology, Immunopathology
Start/End 01.12.2012 - 30.11.2013
Approved amount 505'506.00
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All Disciplines (2)

Discipline
Immunology, Immunopathology
Molecular Biology

Keywords (3)

two-photon microscope; cell trafficking; immune responses

Lay Summary (French)

Lead
Les récents progrès de l'imagerie biologique ont permis le développement de techniques puissantes telles que la microscopie à deux photons qui permet la visualisation in vivo des interactions cellulaires, permettant ainsi de regarder la dynamique des cellules du système immunitaire d’une façon nouvelle.
Lay summary

L'acquisition d'un microscope à deux photons par l'Institut de Recherche en Biomédecine (IRB) permettra aux chercheurs d'étudier en temps réel les événements cellulaires, subcellulaires et moléculaires. Grâce à cette imagerie puissante, les résultats des études jetteront une lumière nouvelle sur les divers aspects de la régulation et sur les fonctions du système immunitaire. Un des premiers projets de recherche prévu sera l’étude de la réaction inflammatoire dans les organes lymphoïdes après vaccination contre la grippe. Il sera alors possible de suivre in vivo l'initiation de la réponse immunitaire et la caractérisation de la dynamique des cellules dans les ganglions lymphatiques après l'administration du vaccin. En plus, il sera également possible de caractériser les mécanismes moléculaires impliqués dans la migration des différents types cellulaires du système immunitaire acquis dans les organes lymphoïdes tels que les plaques de Peyer ou les ganglions lymphatiques. Ces activités de recherches ainsi que celles d'autres futurs utilisateurs apporteront une contribution importante à la compréhension de la pathogenèse ainsi qu’à la découverte de nouvelles voies thérapeutiques pour de nombreuses maladies comme les maladies auto-immunes, le cancer et les maladies infectieuses.


Direct link to Lay Summary Last update: 09.11.2012

Responsible applicant and co-applicants

Publications

Publication
Dynamic intravital imaging of cell-cell interactions in the lymph node
Stein Jens V., F. Gonzalez Santiago (2017), Dynamic intravital imaging of cell-cell interactions in the lymph node, in Journal of Allergy and Clinical Immunology, 139(1), 12-20.
Macrophage Death following Influenza Vaccination Initiates the Inflammatory Response that Promotes Dendritic Cell Function in the Draining Lymph Node
Chatziandreou Nikolaos, Farsakoglu Yagmur, Palomino-Segura Miguel, D’Antuono Rocco, Pizzagalli Diego Ulisse, Sallusto Federica, Lukacs-Kornek Veronika, Uguccioni Mariagrazia, Corti Davide, Turley Shannon J., Lanzavecchia Antonio, Carroll Michael C., Gonzalez Santiago F. (2017), Macrophage Death following Influenza Vaccination Initiates the Inflammatory Response that Promotes Dendritic Cell Function in the Draining Lymph Node, in Cell Reports, 18(10), 2427-2440.

Collaboration

Group / person Country
Types of collaboration
University of Santiago de Compostela Spain (Europe)
- in-depth/constructive exchanges on approaches, methods or results
University of Bern Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
San Raffaele Scientific Institute Italy (Europe)
- in-depth/constructive exchanges on approaches, methods or results

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
The 5th European Academy of Dermatology and Venereology (EADV) Talk given at a conference Role of neutrophils in vaccine immunity 28.11.2013 Bellinzona, Switzerland Fernandez Gonzalez Santiago;


Communication with the public

Communication Title Media Place Year
Talks/events/exhibitions IRB Open Day Italian-speaking Switzerland 2013

Associated projects

Number Title Start Funding scheme
136612 Manipulation of senescence pathways for cancer therapy: from experimental models to clinic 01.02.2012 Ambizione
140963 Purinergic control of adaptive immunity by P2X7 receptor 01.04.2012 Project funding (Div. I-III)
131092 Effector and memory T cell subsets in man and mouse 01.07.2010 Project funding (Div. I-III)
125566 Ornement - entre art et design 01.04.2009 Scientific Conferences
148183 Intravital imaging of polymicrobial respiratory diseases (IMPORED) 01.01.2014 Ambizione
131003 DENANORNA - Dendrimers as nanovectors for targeted siRNA delivery in gene therapy (ERA-Net EuroNanoMedicine) 01.01.2010 ERA-Euro-NanoMed
140704 Conventional and atypical chemokine receptors: different mechanisms of function and common responses 01.06.2012 Project funding (Div. I-III)
137087 Cell Migration 01.09.2011 ProDoc

Abstract

With the present proposal the Institute for Research in Biomedicine (IRB) requests co-funding for the acquisition of a Two-Photon Microscope by the Swiss National Science Foundation. This proposal contains five independent research projects for which such state-of-the-art equipment would be essential. The initiative underlines the leading position of the IRB in life science research and will provide support for other institutions interested in the new technique. The proposed users of the microscope are internationally recognized experts in their respective subspecialties. As outlined in the individual project descriptions, future multiphoton intravital work by these investigators is likely to shed new light on diverse aspects of immune system regulation and function. These activities and those of other future users have the potential to make important contributions to our understanding and therapeutic capabilities in many important diseases.
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