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Multimode Live Imaging - University of Bern (MLI-be)

English title Multimode Live Imaging - University of Bern (MLI-be)
Applicant Heussler Volker
Number 145013
Funding scheme R'EQUIP
Research institution Institut für Zellbiologie Departement Biologie Universität Bern
Institution of higher education University of Berne - BE
Main discipline Cellular Biology, Cytology
Start/End 01.04.2013 - 31.03.2014
Approved amount 214'460.00
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All Disciplines (10)

Discipline
Cellular Biology, Cytology
Embryology, Developmental Biology
Experimental Microbiology
Immunology, Immunopathology
Tropical Medicine
Medical Microbiology
Biochemistry
Zoology
Molecular Biology
Genetics

Keywords (10)

High resolution microscopy; live micrsocopy; parasite-host interaction; cell-cell competition; spinning disc microscopy; laser manipulation; nuclear organization; calcium signaling; cell fate; cell cycle

Lay Summary (German)

Lead
Multifunktionelle Lebendmikroskopie ermöglicht nicht nur das hochauflösende Aufnehmen lebender Gewebe in Echtzeit, sondern erlaubt es auch die Proben zu manipulieren und die dadurch ausgelösten Effekte zu studieren.
Lay summary

Fluoreszierende Markermoleküle und genetisch codierte fluoreszierende Reporterproteine haben in den letzten 30 Jahren die Lichtmikroskopie revolutioniert. Sie ermöglichten es molekulare Ereignisse durch die Anwendung von Lebendmikroskopie in Echtzeit darzustellen. Um allerdings kleinste Strukturen in einer Zelle darstellen zu können, braucht man sehr leistungsstarke Mikroskope mit hoch auflösenden Objektiven. Ausserdem werden sensitive Kameras benötigt, um auch Objekte, die sich schnell bewegen und schwach fluoreszieren, abbilden zu können ohne sie durch eine zu starke Lichtanregung zu beschädigen.

Spinning disk (SD) Mikroskope erlauben diese Analysen, indem sie die Auflösung, Sensitivität und die Geschwindigkeit von CCD Kameras voll ausnutzen. Deswegen ermöglichen SD Mikroskopen Lebendmikroskopie ohne die sonst üblichen Effekte von Ausbleichen der zu untersuchenden Strukturen oder gar Schäden durch eine zu starke Beleuchtung. Bisher war es allerdings nicht möglich die Vorteile der SD Mikroskopie mit in vivo Techniken zu verbinden, die ein Manipulieren der Proben (FRAP, FRET, FLIP, Photoaktivierung/Photoswitch) erlauben.

Das von uns angeschaffte SD Mikroskop hat genau diese Schwierigkeiten überwunden, indem es das hohe Auslösungsvermögen eines Punktscanners mit den ultraschnellen Möglichkeiten einer Weitfeldapperatur verbindet. Es ist das erste SD Mikroskop dieser Art in der Schweiz. Mit diesem innovativen Gerät ist es uns möglich die dynamischen Eigenschaften von zellulären Signalwegen, Zellzyklusregulation, neuronaler Plastizität, subzellulärer Prozesse sowie Parasit-Wirt Interaktionen in lebenden Präparaten zu studieren. Dabei reicht das Spektrum der untersuchten Objekte von der Grösse einer Zelle bis hin zu ganzen Geweben. 


Direct link to Lay Summary Last update: 08.01.2013

Responsible applicant and co-applicants

Publications

Publication
Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D.
Vazquez-Pianzola Paula, Adam Jacqueline, Haldemann Dominique, Hain Daniel, Urlaub Henning, Suter Beat (2014), Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D., in Development (Cambridge, England), 141(9), 1915-26.
Imaging of the spleen in malaria.
Ferrer Mireia, Martin-Jaular Lorena, De Niz Mariana, Khan Shahid M, Janse Chris J, Calvo Maria, Heussler Volker, del Portillo Hernando A (2014), Imaging of the spleen in malaria., in Parasitology international, 63(1), 195-205.
Features of autophagic cell death in Plasmodium liver-stage parasites.
Eickel Nina, Kaiser Gesine, Prado Monica, Burda Paul-Christian, Roelli Matthias, Stanway Rebecca R, Heussler Volker T (2013), Features of autophagic cell death in Plasmodium liver-stage parasites., in Autophagy, 9(4), 568-80.
Mechanisms of cell competition: themes and variations.
Levayer Romain, Moreno Eduardo (2013), Mechanisms of cell competition: themes and variations., in The Journal of cell biology, 200(6), 689-98.
Nuclear organization in the nematode C. elegans.
Sharma Rahul, Meister Peter (2013), Nuclear organization in the nematode C. elegans., in Current opinion in cell biology, 25(3), 395-402.

Collaboration

Group / person Country
Types of collaboration
Andre Schneider, DCB Bern Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
Manfred Heller, University of Bern Switzerland (Europe)
- Research Infrastructure
Rudolf Aebersold, ETH Zürich Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
- Exchange of personnel
Patrick Mathias, FMI Basel Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
- Research Infrastructure
- Exchange of personnel
Hernando del Portillo/University of Barcelona Spain (Europe)
- Publication
Bernhard Schneider, EPFL Lausanne Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
- Exchange of personnel

Scientific events



Self-organised

Title Date Place

Communication with the public

Communication Title Media Place Year
Media relations: print media, online media Gemeinsam gegen Malaria SystemsX Newsletter International Western Switzerland Italian-speaking Switzerland Rhaeto-Romanic Switzerland German-speaking Switzerland 2014

Associated projects

Number Title Start Funding scheme
135436 Composition and dynamics of BicD and Cbp transport particles 01.07.2011 Project funding (Div. I-III)
150824 White-Laser Confocal Microscopy: Modular and Sensitive Multi-channel Analysis 01.12.2013 R'EQUIP
159320 Deciphering the Function of Genome Nuclear Organization in Cell Fate Determination (prolongation) 01.09.2015 SNSF Professorships
159519 Pathogen-host cell interactions during the liver stage of Plasmodium parasites 01.08.2015 Project funding (Div. I-III)
140691 Pathogen-host cell interactions during the liver stage of Plasmodium parasites 01.08.2012 Project funding (Div. I-III)
140779 Mitochondrial biogenesis in Trypanosoma brucei 01.04.2012 Project funding (Div. I-III)
128460 Identification and functional analysis of distinct stress and age related pathways in onset and progression of Spinocerebellar ataxia (SCA1) 01.06.2010 SNSF Professorships
133744 Deciphering the Function of Genome Nuclear Organization in Cell Fate Determination. 01.09.2011 SNSF Professorships
150756 Identification and functional analysis of distinct stress and age related pathways in onset and progression of Spinocerebellar ataxia (SCA1) 01.06.2014 SNSF Professorships
182465 Pathogen-host cell interactions during the liver stage of Plasmodium parasites III 01.01.2019 Project funding (Div. I-III)

Abstract

Confocal microscopy has greatly advanced in the last ten years with the setup of sensitive and efficient spinning disk confocals (SD). These fully exploit the resolution, sensitivity and speed of CCD cameras, overcoming the limitations of classical point- or line-scanning devices. Due to their design, SD microscopes lead to less photodamage and photobleaching, making them ideally suited for live imaging. However, in vivo techniques to study cell dynamics and involving a focused laser beam are impossible to achieve with classical SD designs (FRAP, FRET, FLIP, photoactivation/photoswitch). We propose to setup the first commercially available spinning disk combined with a point laser scan head (SD-scan) in Switzerland to answer a variety of questions involving interaction of the Plasmodium parasite with its host hepatocyte, dynamic features of cell cycle regulation, cell-cell signaling, neuronal plasticity as well as subcellular and subnuclear organization. Ideal for all types of in vivo studies, this SD-scan microscope is able to image life samples ranging in size from intravital liver to subcellular structures.This SD-scan device will be part of the ‘Microscopy and Imaging Center’ (MIC) platform already established at the University of Bern, thus being available to interested researchers inside and outside the University.
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