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Achat d'un appareil Seahorse X-24 pour l'analyse de la fonction respiratoire mitochondriale et d'un instrument de thermophorèse pour l'analyse d'intéractions biomoléculaires

English title Purchase of a Seahorse X-24 for mitochondrial respiration analysis and of a microscale thermophoresis apparatus for the analysis of biomolecular interactions
Applicant Martinou Jean Claude
Number 145001
Funding scheme R'EQUIP
Research institution Département de Biologie Cellulaire Faculté des Sciences Université de Genève
Institution of higher education University of Geneva - GE
Main discipline Cellular Biology, Cytology
Start/End 01.01.2013 - 31.12.2013
Approved amount 134'307.00
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All Disciplines (2)

Discipline
Cellular Biology, Cytology
Biochemistry

Keywords (7)

seahorse ; Thermophorèse; intéractions; affinité; mitochondrie; oxygène; acide lactique

Lay Summary (French)

Lead
Achat d'un appareil Seahorse X-24 pour l'analyse de la fonction respiratoire mitochondriale et d'un instrument de thermophorèse pour l'analyse d'intéractions biomoléculaires
Lay summary
Grâce au subside du Fonds National Suisse, nous pourrons acquérir deux types d’appareils, visant pour l’un, le X24 Analyser (Seahorse), à mesurer des paramètres biologiques de la fonction mitochondriale de cellules en culture, comme par exemple la consommation d’oxygène, et pour l’autre, un appareil de thermophérèse, à mesurer des interactions entre molécules. Ces appareils seront très utiles pour un grand nombre de laboratoires de la communauté scientifique de l’Université de Genève et seront également accessibles à des groupes de recherche d’autres universités suisses.
Direct link to Lay Summary Last update: 16.11.2012

Responsible applicant and co-applicants

Collaboration

Group / person Country
Types of collaboration
Prof. Maechler, Université de Genève Switzerland (Europe)
- Research Infrastructure
Prof. Martinou, Université de Genève Switzerland (Europe)
- Research Infrastructure
Prof. Schibler, Université de Genève Switzerland (Europe)
- Research Infrastructure
Prof. Scapozza, Université de Genève Switzerland (Europe)
- Research Infrastructure
Prof. Picard, Université de Genève Switzerland (Europe)
- Research Infrastructure
Prof. Fitzpatrick, Université de Genève Switzerland (Europe)
- Research Infrastructure
Prof. Karl Heinz Krause Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
Prof. Strub, Université de Genève Switzerland (Europe)
- Research Infrastructure

Associated projects

Number Title Start Funding scheme
127081 Hsp90 molecular chaperone machines in yeast and mammals 01.10.2009 Project funding
126963 Development of PET reporter probes with improved sensitivity and specificity for in vivo monitoring of gene and cell-based therapy using HSV1-tk as a reporter gene 01.10.2009 Project funding
119186 Defining vitamin B1 and B6 metabolism in plants: synthesis, regulation and transport 01.05.2008 SNSF Professorships
133126 Signaling proteins and ion channels of the endoplasmic reticulum, mitochondria, and phagosomes 01.10.2010 Project funding
138058 Human splicing factor SF1: Analysis of nuclear and cytoplasmic functions and definition of regulatory interaction networks 01.10.2011 Project funding
126710 Reactive Oxygen Species in Granzyme-mediated Cell Death: from Mechanism to Applications 01.12.2009 Ambizione
128656 The mammalian circadian timing system: Oscillators, inputs, and outputs 01.01.2010 Project funding
124793 Mechanism and regulation of SRP-dependent protein translocation 01.04.2009 Project funding

Abstract

The goal of this application is to obtain a subsidy for equipping the new Bio-molecular analysis (BMA) platform of the Arc Lémanique Plant Science and the University of Geneva with two instruments: 1)A Seahorse X24 analyser for investigation of mitochondrial respiratory chain function. The cost of this apparatus is CHF 142’614.2)A Microscale Thermophoresis apparatus for measuring biomolecular interactions (prote-in-protein; small molecule-protein; DNA-protein etc.). The cost of this instrument is CHF 126’000.The two instruments will be hosted by the BMA platform at the Faculty of Sciences. This plat-form provides services not only to the Swiss Plant Science Web but also to all departments at the University of Geneva. The new instruments together with the existing equipment will be made accessible to all members of the Geneva scientific community. At the University of Geneva, there are six groups working on cell metabolism and the biology of mitochondria that plan to routinely use the Seahorse analyzer. There are also a high number of groups, of which four are mentioned in this request, that require a technology allowing them to measure molecular interactions with small amounts of material. During a demonstration of the Microscale Thermophoresis apparatus in Geneva in December 2011, members of the four labora-tories participating in this request obtained very encouraging results where classical approaches (i.e. Isothermal microcalorimetry or Surface Plasmon Resonance) had failed. Thus, the acquisiti-on of the Microscale Thermophoresis apparatus would allow many groups of the University of Geneva, and of other universities, to study biomolecular interactions to complement, or in lieu of, the approaches that are currently available at the BMA platform.
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