Stem cells, muscles, Tissue engineering; GMP, clinical trial
Eberli Daniel, Mortezavi Ashkan, Sulser Tullio (2014), [Focal therapy-a new era in the treatment of prostate cancer]., in Praxis
, 103(7), 391-7.
Brügger Lukas, Inglin Roman, Candinas Daniel, Sulser Tullio, Eberli Daniel (2014), A novel animal model for external anal sphincter insufficiency., in International journal of colorectal disease
, 29(11), 1385-92.
Hermanns Thomas, Gross Oliver, Kranzbühler Benedikt, Hefermehl Lukas J, Poyet Cédric, Müller Alexander, Yap Stanley A, Michel Maurice S, Eberli Daniel, Müntener Michael, Zimmermann Matthias, Sulser Tullio, Seifert Hans-Helge (2014), Ablative efficiency of 532-nm laser vaporization compared to transurethral resection of the prostate: results from a prospective three-dimensional ultrasound volumetry study., in World journal of urology
, 32(5), 1267-74.
Tremp Mathias, Salemi Souzan, Largo Remo, Andersson Karl-Erik, A Plock Jan, Aboushwareb Tamer, Sulser Tullio, Eberli Daniel (2014), Adipose-derived stem cells (ADSCs) and muscle precursor cells (MPCs) for the treatment of bladder voiding dysfunction., in World journal of urology
, 32(5), 1241-8.
Tremp Mathias, Salemi Souzan, Largo Remo, Andersson Karl-Erik, Plock Jan, Aboushwareb Tamer, Sulser Tullio, Eberli Daniel (2014), Erratum to: Adipose-derived stem cells (ADSCs) and muscle precursor cells (MPCs) for the treatment of bladder voiding dysfunction., in World journal of urology
, 32(5), 1249-1249.
Valerio Massimo, Emberton Mark, Barret Eric, Eberli Daniel, Eggener Scott E, Ehdaie Behfar, Jichlinski Patrice, Ward John F, Ahmed Hashim U (2014), Health technology assessment in evolution - focal therapy in localised prostate cancer., in Expert review of anticancer therapy
Hefermehl Lukas J, Largo Remo A, Hermanns Thomas, Poyet Cédric, Sulser Tullio, Eberli Daniel (2014), Lateral temperature spread of monopolar, bipolar and ultrasonic instruments for robot-assisted laparoscopic surgery., in BJU international
, 114(2), 245-52.
Horst Maya, Madduri Srinivas, Milleret Vincent, Sulser Tullio, Gobet Rita M., Eberli Daniel (2013), A bilayered hybrid microfibrous PLGA-Acellular matrix scaffold for hollow organ tissue engineering, in Biomaterials
, 34(5), 1537-1545.
Schweizer Dennis F, Schweizer Riccardo, Zhang Shengye, Kamat Pranitha, Contaldo Claudio, Rieben Robert, Eberli Daniel, Giovanoli Pietro, Erni Dominique, Plock Jan A (2013), Botulinum toxin A and B raise blood flow and increase survival of critically ischemic skin flaps., in The Journal of surgical research
, 184(2), 1205-13.
Reiner Caecilia S, Roessle Matthias, Thiesler Thore, Eberli Daniel, Klotz Ernst, Frauenfelder Thomas, Sulser Tullio, Moch Holger, Alkadhi Hatem (2013), Computed tomography perfusion imaging of renal cell carcinoma: systematic comparison with histopathological angiogenic and prognostic markers., in Investigative radiology
, 48(4), 183-91.
Mortezavi Ashkan, Hermanns Thomas, Hefermehl Lukas J., Spahn Donat Rudolf, Seifert Burkhardt, Weber Damian, Brunnschweiler Simone, Schmid Daniel Max, Sulser Tullio, Eberli Daniel (2013), Continuous low-dose aspirin therapy in robotic-assisted laparoscopic radical prostatectomy does not increase risk of surgical hemorrhage, in Journal of Laparoendoscopic and Advanced Surgical Techniques
, 23(6), 500-505.
Benz Sebastian, Nötzli Sarah, Siegel Jay S, Eberli Daniel, Jessen Henning J (2013), Controlled oxygen release from pyridone endoperoxides promotes cell survival under anoxic conditions., in Journal of medicinal chemistry
, 56(24), 10171-82.
Horst Maya, Madduri Srinivas, Gobet Rita M., Sulser Tullio, Milleret Vinzent, Hall Heike Kenton, Atala Anthony, Eberli Daniel (2013), Engineering functional bladder tissues, in Journal of Tissue Engineering and Regenerative Medicine
, 7(7), 515-522.
Burnett Luke R., Rahmany Mária B., Richter Jillian R., Aboushwareb Tamer A., Eberli Daniel, Ward Catherine L., Orlando Giuseppe, Hantgan Roy R., Van Dyke Mark E. (2013), Hemostatic properties and the role of cell receptor recognition in human hair keratin protein hydrogels, in Biomaterials
, 34(11), 2632-2640.
Maya Horst, Vincent Milleret, Nötzli Sarah, Madduri Srinivas, Tullio Sulser, Rita Gobet, Eberli Daniel (2013), Increased porosity of electrospun hybrid scaffolds improved bladder tissue regeneration, in Journal of Biomedical Materials Research - Part A
Binder Carmen, Milleret Vincent, Hall Heike, Eberli Daniel, Lühmann Tessa (2013), Influence of micro and submicro poly(lactic-glycolic acid) fibers on sensory neural cell locomotion and neurite growth., in Journal of biomedical materials research. Part B, Applied biomaterials
, 101(7), 1200-8.
Winklehner Anna, Blume Iris, Winklhofer Sebastian, Eberli Daniel, Gnannt Ralph, Frauenfelder Thomas, Alkadhi Hatem (2013), Iterative reconstructions versus filtered back-projection for urinary stone detection in low-dose CT., in Academic radiology
, 20(11), 1429-35.
Horst Maya, Heutschi Janine, Van Den Brand Rubia J., Andersson Karl Erik, Gobet Rita M., Sulser Tullio, Courtine Grégoire, Eberli Daniel (2013), Multisystem neuroprosthetic training improves bladder function after severe spinal cord injury, in Journal of Urology
, 189(2), 747-753.
Stölting, Ferrari Stefano, Handschin Christoph, Becskei Attila, Provenzano Maurizio, Sulser Tullio, Eberli Daniel (2013), Myoblasts inhibit prostate cancer growth by paracrine secretion of tumor necrosis factor-α, in Journal of Urology
, 189(5), 1952-1959.
Azzabi Fahd (2013), Skeletal Muscle Regeneration for Clinical Application, in Andrades Jose A (ed.), INTECH, Rijeka, Ch27.
Karlo CA Gnannt R Winklehner A Fischer MA Donati OF Eberli D Sulser T Alkadhi H Stolzmann P. (2013), Split-bolus dual-energy CT urography: protocol optimization and diagnostic performance for the detection of urinary stones, in Abdomianl Imaging
Gnannt Ralph, Winklehner Anna, Eberli Daniel, Knuth Alexander K., Frauenfelder Thomas, Alkadhi Hatem (2012), Automated tube potential selection for standard chest and abdominal CT in follow-up patients with testicular cancer: Comparison with fixed tube potential, in European Radiology
, 22(9), 1937-1945.
Schuler V, Suter K, Fürer K, Eberli D, Horst M, Betschart C, Brenneisen R, Hamburger M, Mennet M, Schnelle M, Simões-Wüst A P, von Mandach U (2012), Bryophyllum pinnatum inhibits detrusor contractility in porcine bladder strips--a pharmacological study towards a new treatment option of overactive bladder., in Phytomedicine : international journal of phytotherapy and phytopharmacology
, 19(10), 947-51.
Milleret Vincent, Hefti Thomas, Hall Heike, Vogel Viola, Eberli Daniel (2012), Influence of the fiber diameter and surface roughness of electrospun vascular grafts on blood activation, in ACTA BIOMATERIALIA
, 8(12), 4349-4356.
Mortezavi, Hermanns Thomas, Seifert Hans Helge, Wild Peter J., Schmid Daniel Max, Sulser Tullio, Eberli Daniel (2012), Intrafascial dissection significantly increases positive surgical margin and biochemical recurrence rates after robotic-assisted radical prostatectomy, in Urologia Internationalis
, 89(1), 17-24.
Eberli Daniel, Aboushwareb Tamer, Soker Shay, Yoo James J., Atala Anthony (2012), Muscle Precursor Cells for the Restoration of Irreversibly Damaged Sphincter Function, in CELL TRANSPLANTATION
, 21(9), 2089-2098.
Schlosser Stefan, Dennler Cyrill, Schweizer Riccardo, Eberli Daniel, Stein Jens V., Enzmann Volker, Giovanoli Pietro, Erni Dominique, Plock Jan Alexander (2012), Paracrine effects of mesenchymal stem cells enhance vascular regeneration in ischemic murine skin, in Microvascular Research
, 83(3), 267-275.
Schweizer Riccardo, Kamat Pranitha, Schweizer Dennis, Dennler Cyrill, Zhang Shengye, Schnider Jonas T, Salemi Souzan, Giovanoli Pietro, Eberli Daniel, Enzmann Volker, Erni Dominique, Plock Jan A, Bone marrow-derived mesenchymal stromal cells improve vascular regeneration and reduce leukocyte-endothelium activation in critical ischemic murine skin in a dose-dependent manner., in Cytotherapy
Jaatinen L, Salemi S, Miettinen S, Hyttinen J, Eberli D, The Combination of Electric Current and Copper Promotes Neuronal Differentiation of Adipose-Derived Stem Cells., in Annals of biomedical engineering
Stölting Meline N L, Hefermehl Lukas J, Tremp Mathias, Azzabi Fahd, Sulser Tullio, Eberli Daniel, The role of donor age and gender in the success of human muscle precursor cell transplantation., in Journal of tissue engineering and regenerative medicine
Azzabi Fahd, Rottmar Markus, Jovaisaite Virginija, Rudin Markus, Sulser Tullio, Boss Andreas, Eberli Daniel, Viability, Differentiation Capacity, and Detectability of Super-Paramagnetic Iron Oxide-Labeled Muscle Precursor Cells for Magnetic-Resonance Imaging., in Tissue engineering. Part C, Methods
Grant Prolongation, Please see also „Cover letter and Applicaiton“ Urinary incontinence, the involuntary loss of urine, still remains a mayor medical issue with approximately 20% of women affected. The quality of life of patients with urinary incontinence is severely affected and the incurring healthcare costs are significant. The main cause for stress urinary incontinence (SUI) is damage of the sphincter muscle, which occurs during childbirth, surgical treatments or as an effect of aging. Current surgical treatment options enable recovery of continence with various outcomes. However, definitive correction of underlying etiology has not been accomplished. Furthermore, these options only offer short-term relief and the overall success of these therapies are limited by complications.Recently, we were able to publish our results on the effectiveness of using autologous muscle precursor cells (MPCs) in the treatment of sphincter insufficiency in a large animal model. Our results showed that injected muscle cells were able to form new sphincter muscle and achieve 80% of sphincter function after 6 months. Over the last 2.5 years we have followed the time line outlined in the SCORE proposal and have gained substantial research data supporting an clincial trial. The University of Zurich and the Department of Urology have committed to the formation of a laboratory for urologic tissue engineering and a good manufacturing practice (GMP) laboratory, supported by Swiss national fund (SNF), will be certified soon.We have proposed a research plan with the ultimate goal of conducting a phase I clinical trial within four years. We envision initiating clinical trials in 2013, starting with sphincter reconstruction, using a cell-based strategy. The approach presented here was chosen due to the simple and minimal invasive nature of the procedure for the functional restoration of the urinary sphincter. The rationale for implanting muscle into the sphincter region is two-fold. First, the generation of a muscle tissue mass within the bladder neck region will achieve a bulking effect that would increase the sphincter pressure, thus controlling incontinence. Second, implantation of muscle cells will achieve restoration of sphincter function through innervation, which control continence and micturation over time. Simultaneously to preparing the clinical trial, we will pursue further scientific investigations and preclinical studies necessary in the future to applying genetic regenerative approach to the restoration of the SUI.We hypothesize that autologous MPCs injected into damaged sphincter muscles of patients with urinary incontinence are able to form new functional muscle tissue, support sphincter function and significantly improve the quality of life in human, as it has been the case in our large animal model. We will test this hypothesis in the following specific aims and, in this application, we ask for support to complete these aims. Aim 1: Optimize human muscles cells procedures to restore the urinary sphinctera-Define a reliable human muscle cell culture system for human muscle cell therapyb-Analyze engineered human muscle for functional tissue restorationc-Determine the donor age and gender limitations for the use of autologous muscle cellsd-Determine if genetically modified muscle precursor cells improve sphincter muscle functionAim 2: Evaluation of imaging guided minimal invasive techniques for muscle cell injectiona-Determine the feasibility of minimal invasive techniques for MPC injection using different imaging modalitiesb-Construct a needle guidance that satisfies international requirements for medical devicesc-Evaluate the use of nanoparticles for in vivo cell trackingAim 3: Restore damaged sphincter muscle function using muscle cell therapy in patients: phase I + II Triala-Establish a reproducible procedure for cellular therapy of urinary incontinence in patients respecting GMP and good clinical practices (GCP) requirementsb-Analyze the regenerated sphincter muscle tissue using non-invasive imaging techniquesc-Evaluate the clinical impact of MPC therapy using standard clinical assessment