Project

Back to overview

The Bacterial Lectin FimH - High and Low Affinity States

Applicant Ernst Beat
Number 144183
Funding scheme Project funding (Div. I-III)
Research institution Institut für Molekulare Pharmazie Pharmazentrum Universität Basel
Institution of higher education University of Basel - BS
Main discipline Biophysics
Start/End 01.10.2012 - 30.09.2015
Approved amount 405'000.00
Show all

All Disciplines (3)

Discipline
Biophysics
Organic Chemistry
Molecular Biology

Keywords (12)

Recombinant protein production; FimH antagonists; Pharmacokinetics; Pharmacodynamics; Synthesis of carbohydrates & mimics; kinetics and thermodynamics of protein folding; X-ray crystallography; Protein folding and assembly; Structural biology; Protein biochemistry; Protein biophysics

Responsible applicant and co-applicants

Employees

Publications

Publication
FimH Antagonists: Bioisosteres To Improve the in Vitro and in Vivo PK/PD Profile
Kleeb Simon, Pang Lijuan, Mayer Katharina, Eris Deniz, Sigl Anja, Preston Roland C., Zihlmann Pascal, Sharpe Timothy, Jakob Roman P., Abgottspon Daniela, Hutter Aline S., Scharenberg Meike, Jiang Xiaohua, Navarra Giulio, Rabbani Said, Smiesko Martin, Luedin Nathalie, Bezencon Jacqueline, Schwardt Oliver, Maier Timm, Ernst Beat (2015), FimH Antagonists: Bioisosteres To Improve the in Vitro and in Vivo PK/PD Profile, in JOURNAL OF MEDICINAL CHEMISTRY, 58(5), 2221-2239.
The Tyrosine Gate of the Bacterial Lectin FimH: A Conformational Analysis by NMR Spectroscopy and X-ray Crystallography
Fiege Brigitte, Rabbani Said, Preston Roland C., Jakob Roman P., Zihlmann Pascal, Schwardt Oliver, Jiang Xiaohua, Maier Timm, Ernst Beat (2015), The Tyrosine Gate of the Bacterial Lectin FimH: A Conformational Analysis by NMR Spectroscopy and X-ray Crystallography, in CHEMBIOCHEM, 16(8), 1235-1246.
Catch-bond mechanism of the bacterial adhesin FimH
Maximilian M. Sauer Roman P. Jakob Jonathan Eras Sefer Baday Deniz Eris Giulio Navarra Simon B, Catch-bond mechanism of the bacterial adhesin FimH, in Nature Communications.

Collaboration

Group / person Country
Types of collaboration
Prof. Thomas Peters, Universität Lübeck Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Dr. Nina Khanna, University of Basel Switzerland (Europe)
- Publication

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
51st Winter Seminar on Biophysical Chemistry, Molecular Biology and Cybernetics of Cell Functions Talk given at a conference Assembly of adhesive type 1 pili from pathogenic Escherichia coli and mechanism of shear force dependent receptor binding 07.01.2016 Klosters, Switzerland Glockshuber Rudolf;
PacifiChem 2015 Talk given at a conference Druggability of Lectins, Using the Example of a Bacterial Adhesin 16.12.2015 Honolulu, United States of America Ernst Beat;
Biochemisches Kolloquium Universität Leipzig Talk given at a conference Assembly of adhesive type 1 pili from uropathogenic E. coli strains and mechanism of shear force dependent adhesion 08.12.2015 Leipzig, Germany Glockshuber Rudolf;
Glycobiology Annual Meeting Talk given at a conference Druggability of Lectins, as Exemplified by a Bacterial Adhesin 04.12.2015 San Francisco, United States of America Ernst Beat;
Actelion Individual talk Anti-Adhesion Therapy to Prevent Urinary Tract Infections (UTI) 16.11.2015 Allschwil, Switzerland Ernst Beat;
26th Faltertage on Protein Folding, Dynamics and Stability; Martin-Luther –Universität Halle-Wittenberg Talk given at a conference Mechanism of Catch Bond Formation by the adhesin FimH from uropathogenic E. coli strains 24.10.2015 Halle, Germany Glockshuber Rudolf;
EuroCarb18 Talk given at a conference FIMH ANTAGONISTS AS A NOVEL APPROACH FOR THE PREVENTION AND TREATMENT OF URINARY TRACT INFECTIONS 02.08.2015 Moskau, Russia Eris Deniz;
EuroCarb18 Talk given at a conference From Carbohydrates to Glycomimetics – Is There a General Recipe? 02.08.2015 Moskau, Russia Ernst Beat;
Bundle Symposium Talk given at a conference Druggability of Lectins – Mission Possible? 16.07.2015 Edmonton, Canada Ernst Beat;
Institute Pasteur Individual talk How bacteria hold tight in bladder infection 30.06.2015 Paris, France Jakob Roman;
Basel Infectious Diseases Study Club Talk given at a conference Anti-Adhesion Therapy for Urinary Tract Infections - A Balanced PK/PD-Profile Proved to be Key for Success 28.05.2015 Basel, Switzerland Ernst Beat;
Conformational Transitions in Proteins (Organized by: Technical University of Munich) Talk given at a conference Type 1 pili from uropathogenic Escherichia coli: Mechanism of assembly and shear force dependent adhesion to target cells 05.05.2015 Venedig, Italy Glockshuber Rudolf;
European Congress of Clinical Microbiology and Infectious Diseases Talk given at a conference Antivirulence Pharmaceutical Approaches to Prevent UTI 25.04.2015 Kopenhagen, Denmark Ernst Beat;
Basel Postdoc Network Retreat 2014 Talk given at a conference How bacteria hold tight in bladder infection 26.09.2014 Engelberg, Switzerland Jakob Roman;
Gastseminar Novartis Individual talk Conformational Changes in the Recognition of Special Ligands : Catch-bond Lectins and ?-Barrel Membrane Protein Insertion 14.01.2014 Basel, Switzerland Maier Timm;


Associated projects

Number Title Start Funding scheme
129935 From Oligosaccharides to Glycomimetics to Glycodrugs 01.04.2010 Project funding (Div. I-III)
159696 Multienzymes in Lipid and Polyketide Biosynthesis 01.04.2015 Project funding (Div. I-III)
120904 Antagonists of Type 1 Fimbriae-mediated Adhesion of Escherichia coli: Treatment of Urinary Tract Infections 01.04.2008 Interdisciplinary projects
138657 Mechanismus der katalysierten Assemblierung supramolekularer Komplexe am Modell der Assemblierungsplattform FimD von Typ 1 Pili von pathogenen Escherichia coli Stämmen 01.10.2011 Project funding (Div. I-III)
156304 Functional significance of the dynamics of receptor binding and the alternative folding possibilities of pilus subunits in urinary tract infections caused by pathogenic Escherichia coli strains 01.10.2014 Project funding (Div. I-III)
138262 Multienzymes and the Regulation of Eukaryotic Lipid Metabolism 01.01.2012 Project funding (Div. I-III)

-