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Molecular mechanisms of interaction between herpes simplex virus type 1 and adeno-associated virus type 2 in co-infected cells

English title Molecular mechanisms of interaction between herpes simplex virus type 1 and adeno-associated virus type 2 in co-infected cells
Applicant Fraefel Cornel
Number 144094
Funding scheme Project funding
Research institution Virologisches Institut Universität Zürich
Institution of higher education University of Zurich - ZH
Main discipline Experimental Microbiology
Start/End 01.07.2013 - 30.06.2016
Approved amount 484'827.00
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Keywords (5)

Herpes simplex virus type 1 (HSV-1); Adeno-associated virus (AAV); virus-virus interaction; virus-cell interaction; replication

Responsible applicant and co-applicants

Employees

Publications

Publication
Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1
Franzoso Francesca D., Seyffert Michael, Vogel Rebecca, Yakimovich Artur, de Andrade Pereira Bruna, Meier Anita F., Sutter Sereina O., Tobler Kurt, Vogt Bernd, Greber Urs F., Büning Hildegard, Ackermann Mathias, Fraefel Cornel (2017), Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1, in Journal of Virology, 91(15), e00357-17.
Novel Mutant AAV2 Rep Proteins Support AAV2 Replication without Blocking HSV-1 Helpervirus Replication
Seyffert Michael, Glauser Daniel L., Schraner Elisabeth M., de Oliveira Anna-Paula, Mansilla-Soto Jorge, Vogt Bernd, Büning Hildegard, Linden R. Michael, Ackermann Mathias, Fraefel Cornel (2017), Novel Mutant AAV2 Rep Proteins Support AAV2 Replication without Blocking HSV-1 Helpervirus Replication, in PLOS ONE, 12(1), e0170908-e0170908.
Adeno-Associated Virus Type 2 Rep68 Can Bind to Consensus Rep-Binding Sites on the Herpes Simplex Virus 1 Genome.
Michael Seyffert, Daniel L. Glauser, Kurt Tobler, Oleg Georgiev, Rebecca Vogel, Bernd Vogt, Laetitia Agundez, Michael Linden, Hildegard Büning, Mathias Ackermann, Cornel Fraefel, Michael Seyffert, Daniel L. Glauser, Kurt Tobler, Oleg Georgiev, Rebecca Vogel, Bernd Vogt, Laetitia Agundez, Michael Linden, Hildegard Büning, Mathias Ackermann, Cornel Fraefel, Michael Seyffert, Daniel L. Glauser, Kurt Tobler, Oleg Georgiev, Rebecca Vogel, Bernd Vogt, Laetitia Agundez, Michael Linden, Hildegard Büning, Mathias Ackermann, Cornel Fraefel, Michael Seyffert, Daniel L. Glauser, Kurt Tobler, Oleg Georgiev, Rebecca Vogel, Bernd Vogt, Laetitia Agundez, Michael Linden, Hildegard Büning, Mathias Ackermann, Cornel Fraefel (2015), Adeno-Associated Virus Type 2 Rep68 Can Bind to Consensus Rep-Binding Sites on the Herpes Simplex Virus 1 Genome., in Journal of Virology, 11150.
Viral and Cellular Components of AAV2 Replication Compartments.
Vogel Rebecca, Seyffert Michael, Pereira Bruna de Andrade, Fraefel Cornel, Vogel Rebecca, Seyffert Michael, Pereira Bruna de Andrade, Fraefel Cornel, Vogel Rebecca, Seyffert Michael, Pereira Bruna de Andrade, Fraefel Cornel, Vogel Rebecca, Seyffert Michael, Pereira Bruna de Andrade, Fraefel Cornel (2013), Viral and Cellular Components of AAV2 Replication Compartments., in The open virology journal, 7, 98-120.

Collaboration

Group / person Country
Types of collaboration
Prof. Dr. Hildegard Büning Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Prof. Dr. Urs Greber Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
- Exchange of personnel
Prof. Dr. Michael Linden, King's College, London Great Britain and Northern Ireland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Dr. Artur Yakimovich Great Britain and Northern Ireland (Europe)
- in-depth/constructive exchanges on approaches, methods or results

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
16th International Parvovirus Workshop Poster Global gene expression analysis of adeno-associated virus type 2 (AAV2)-infected cells 19.06.2016 Ajaccio, France Ackermann Mathias; Fraefel Cornel;
16th International Parvovirus Workshop Talk given at a conference Restricted siRNA screen reveals differential effects of cellular proteins on gene expression from single-stranded and double-stranded AAV2 vectors 19.06.2016 Ajaccio, France Franzoso Francesca; Fraefel Cornel; Vogel Rebecca; Ackermann Mathias;
Swiss Society for Microbiology Annual Meeting 2016 Talk given at a conference Restricted siRNA screen reveals differential effects of cellular proteins on gene expression from single-stranded and double-stranded AAV2 vectors 13.06.2016 Bern, Switzerland Franzoso Francesca;
6th Swiss Virology Meeting Poster Global gene expression analysis of adeno-associated virus type 2 (AAV2)-infected cells 03.02.2016 Thun, Switzerland Fraefel Cornel; Ackermann Mathias;
University of Medicine and Pharmacy, Targu Mures, Romania Individual talk Molecular and Applied Virology 18.03.2015 Targu Mures, Romania Fraefel Cornel;
5th Swiss Virology Meeting Poster RNA interference screen reveals cellular factors involved in HSV-1-supported AAV2 replication 09.09.2014 Thun, Switzerland Vogel Rebecca; Ackermann Mathias; Franzoso Francesca; Fraefel Cornel;
5th Swiss Virology Meeting Poster AAV2 Rep68 Can Bind to Consensus Rep-binding Sites on the HSV-1 Genome 09.09.2014 Thun, Switzerland Ackermann Mathias; Vogel Rebecca; Seyffert Michael; Fraefel Cornel;
15th International Parvovirus Workshop Talk given at a conference Replication of HSV-1 and AAV2 in co-infected cells depends on the cell cycle 22.06.2014 Bordeaux, France Seyffert Michael; Franzoso Francesca; Vogel Rebecca; Fraefel Cornel; Ackermann Mathias;
University of Bern, Vetsuisse Faculty Individual talk Molecular mechanisms of interaction between adeno-associated virus and its helperviruses in co-infected cells 24.04.2014 Bern, Switzerland Fraefel Cornel;


Self-organised

Title Date Place
6th Swiss Virology Meeting 03.02.2016 Thun, Switzerland
5th Swiss Virology Meeting 09.09.2014 Thun, Switzerland

Communication with the public

Communication Title Media Place Year
Print (books, brochures, leaflets) Allgemeine Virologie International German-speaking Switzerland 2016

Associated projects

Number Title Start Funding scheme
166668 Molecular mechanisms of interaction between herpes simplex virus type 1 and adeno-associated virus 2 in co-infected cells 01.07.2016 Project funding
112462 Alternative and competing viral replication origins: analysis of the molecular mechanisms of interaction 01.08.2006 Project funding
184766 Molecular mechanisms of interaction between herpes simplex virus type 1 and adeno-associated virus 2 in co-infected cells 01.07.2019 Project funding
124938 Alternative and competing viral replication origins: analysis of the molecular mechanisms of interaction between herpes simplex virus type 1 and adeno-associated virus in co-infected cells 01.03.2010 Project funding
166668 Molecular mechanisms of interaction between herpes simplex virus type 1 and adeno-associated virus 2 in co-infected cells 01.07.2016 Project funding
170799 High content confocal imaging of cellular phenotypes in health and disease 01.12.2016 R'EQUIP

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