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The Body on a Chip - Human 3-D tissue analogues in microfluidic systems

Applicant Frey Olivier
Number 142440
Funding scheme Ambizione
Research institution Computational Systems Biology Department of Biosystems, D-BSSE ETH Zürich
Institution of higher education ETH Zurich - ETHZ
Main discipline Microelectronics. Optoelectronics
Start/End 01.04.2013 - 31.03.2016
Approved amount 455'278.00
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All Disciplines (5)

Discipline
Microelectronics. Optoelectronics
Biomedical Engineering
Molecular Biology
Cellular Biology, Cytology
Mechanical Engineering

Keywords (5)

Microfluidic; 3D Tissue Engineering; Drug development; Biosensor; Body on a chip

Responsible applicant and co-applicants

Employees

Publications

Publication
Seamless Combination of Fluorescence-Activated Cell Sorting and Hanging-Drop Networks for Individual Handling and Culturing of Stem Cells and Microtissue Spheroids
Birchler Axel, Berger Mischa, Jäggin Verena, Lopes Telma, Etzrodt Martin, Misun Patrick, Pena Maria, Schroeder Timm, Hierlemann Andreas, Frey Olivier (2016), Seamless Combination of Fluorescence-Activated Cell Sorting and Hanging-Drop Networks for Individual Handling and Culturing of Stem Cells and Microtissue Spheroids, in Analytical Chemistry, 88(2), 1222-1229.
3D spherical microtissues and microfluidic technology for multi-tissue experiments and analysis.
Kim Jin-Young, Fluri David A, Marchan Rosemarie, Boonen Kurt, Mohanty Soumyaranjan, Singh Prateek, Hammad Seddik, Landuyt Bart, Hengstler Jan G, Kelm Jens M, Hierlemann Andreas, Frey Olivier (2015), 3D spherical microtissues and microfluidic technology for multi-tissue experiments and analysis., in Journal of biotechnology, 1.
Adding the ‘heart’ to hanging drop networks for microphysiological multi-tissue experiments
Rismani Yazdi Saeed, Shadmani Amir, Bürgel Sebastian, Misun Patrick, Hierlemann Andreas, Frey Olivier (2015), Adding the ‘heart’ to hanging drop networks for microphysiological multi-tissue experiments, in Lab on a Chip, 15(21), 4138-4147.
96-Well Format-Based Microfluidic Platform for Parallel Interconnection of Multiple Multicellular Spheroids.
Kim Jin-Young, Fluri David A, Kelm Jens M, Hierlemann Andreas, Frey Olivier (2014), 96-Well Format-Based Microfluidic Platform for Parallel Interconnection of Multiple Multicellular Spheroids., in Journal of laboratory automation, 1.
Reconfigurable microfluidic hanging drop network for multi-tissue interaction and analysis.
Frey Olivier, Misun Patrick M, Fluri David A, Hengstler Jan G, Hierlemann Andreas (2014), Reconfigurable microfluidic hanging drop network for multi-tissue interaction and analysis., in Nature communications, 5, 4250-4250.
Multi-Analyte Biosensor Interface for Real-Time Monitoring of 3D Microtissue Spheroids in Hanging-Drop Networks
Patrick Misun, Yannick Schmid, Jörg Rothe, Andreas Hierlemann, Olivier Frey, Multi-Analyte Biosensor Interface for Real-Time Monitoring of 3D Microtissue Spheroids in Hanging-Drop Networks, in Microsystems & Nanoengineering.

Collaboration

Group / person Country
Types of collaboration
Vrije Universiteit Brussel, Leo van Grunsven, Liver Cell Biology Laboratory Belgium (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Saarland University, Thorsten Lehr, Professor of Clinical Pharmacy Germany (Europe)
- in-depth/constructive exchanges on approaches, methods or results
ETH Zurich, D-BSSE Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
InSphero AG, Zurich Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
DECHEMA 3D Cell Culture Conference Talk given at a conference Adding metabolic competence to 3D spheroid-based assays by using a microfluidic multi-tissue culturing platform 19.04.2016 Freiburg, Germany Frey Olivier;
DECHEMA 3D Cell Culture Conference Poster Automated impedance analysis of drug impact on cancer spheroids 19.04.2016 Freiburg, Germany Frey Olivier;
DECHEMA 3D Cell Culture Conference Poster Biosensor interface for real-time monitoring of 3D 164 microtissue spheroids in hanging-drop networks 19.04.2016 Freiburg, Germany Frey Olivier;
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS) Talk given at a conference Integrated Pump for Hanging-Drop Networks Controlled by Human iPS-Derived Cardiac Microtissue 25.10.2015 Gyeongju, Korean Republic (South Korea) Frey Olivier;
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS) Poster Seamless Combination of FACS and Microfluidic Culturing of Cell Suspensions using the Hanging Drop Network Technology 25.10.2015 Gyeongju, Korean Republic (South Korea) Frey Olivier;
EUSAAT 2015 Talk given at a conference Pro-drug activation in a dynamic microfluidic system interconnecting liver and tumor microtissues 25.09.2015 Linz, Austria Frey Olivier;
EUSAAT 2015 Talk given at a conference Microfluidic in-vitro platform for parallel human multi-tissue interaction studies 25.09.2015 Linz, Austria Frey Olivier;
18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers) Poster Real-time Multi-analyte online Monitoring of 3D cell Cultures by integrated Enzyme-Based Biosensors in Hanging Drop Networks 21.06.2015 Anchorage, United States of America Frey Olivier;
54th Annual Meeting of Society of Toxicology Poster Prodrug Activation in a Dynamic Microfluidics System Interconnecting Liver and Tumor Microtissues 22.03.2015 San Diego, United States of America Frey Olivier;
4th Annual Conference of the Society for Laboratory Automation and Screening Talk given at a conference Microtissues Meet Microfluidics: A Body on a Chip Platform Technology Based on a Highly Parallel Standard SBS Layout 07.02.2015 Washington, United States of America Frey Olivier;
28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS) Poster Microfluidic hanging-drop platform for parallel closed-loop multi-tissue experiments 18.01.2015 Estoril, Portugal Frey Olivier;
Micro and Nano Engineering Conference Talk given at a conference Integration of a Peristaltic Micropump into Microfluidic Hanging Drop Networks 22.11.2014 Lausanne, Switzerland Frey Olivier;
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences Poster Liver-Tumor Model based on Spheroids in Microfluidic Networks for Pharmacokinetic Studies 26.10.2014 San Antonio, United States of America Frey Olivier;
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences Talk given at a conference Monitoring of 3D Multi-cellular Spheroids in Hanging Drop Networks through in-situ Impedance Spectroscopy 26.10.2014 San Antonio, United States of America Frey Olivier;
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS) Poster 96 well format-based microfludiic platform for multiorgan network using multicellular spheroids 25.10.2014 Gyeongju, Korean Republic (South Korea) Frey Olivier;
Eurosensors Conference Talk given at a conference Real-time In-situ Lactate Monitoring in 3D Multi-Cellular Spheroid Cultures by using Enzyme-Based Biosensors in Hanging Drop Networks 07.10.2014 Brescia, Italy Frey Olivier;
9th World Congress on Alternatives and Animal Use in the Life Sciences 2014 Talk given at a conference Microtissues meet microfluidic: A multi-parallel body on a chip concept 24.08.2014 Prag, Czech Republic Frey Olivier;
9th World Congress on Alternatives and Animal Use in the Life Sciences 2014, August 24-28 Talk given at a conference Developments towards physiologic in vitro models in Switzerland 24.08.2014 Prag, Czech Republic Frey Olivier;
DECHEMA 3D Cell Culture Conference Talk given at a conference Connecting hanging drops in a microfluidic network for compound testing in
 continuously perfused multi-tissue formats 25.06.2014 Freiburg, Germany Frey Olivier;
DECHEMA 3D Cell Culture Conference Poster Simple and robust microfluidic platform for spheroid culturing 25.06.2014 Freiburg, Germany Frey Olivier;
28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS) Talk given at a conference Simultaneous impedance spectroscopy and stimulation of human iPS-derived cardiac 3D spheroids in hanging-drop networks 18.01.2014 Estoril, Portugal Frey Olivier;
The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences Talk given at a conference HANGING MICROFLUIDICS: A HIGHLY VERSATILE PLATFORM FOR PRODUCTION AND CULTIVATION OF 3D SPHERICAL MICROTISSUES 27.10.2013 Freiburg, Germany Frey Olivier;
2nd Swiss Image-Based Screening Conference Talk given at a conference A Hanging Microfluidic Platform for 3D Spherical Microtissues: Culturing and Imaging Approaches for Multi-Cell-Based Assay 10.06.2013 Lausanne, Switzerland Frey Olivier;


Communication with the public

Communication Title Media Place Year
Media relations: print media, online media Microfluidic device lets the drop beat Chemistry World International 2015
Media relations: print media, online media Organsystem in hängenden Tropfen ETH Zurich International 2014

Awards

Title Year
Highly Commended Award (£5000) in the 3Rs Prize competition 2014 of the British National Center for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) for the paper entitled "Reconfigurable microfluidic hanging drop network for multi-tissue interaction and analysis" in Nature Communications. https://www.nc3rs.org.uk/3rsprize 2014

Associated projects

Number Title Start Funding scheme
164087 Lightsheet Microscopy 01.06.2016 R'EQUIP

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