gametophyte; genomic imprinting; epigenetics; maternal effects; arabidopsis; fertilization
Kessler Sharon A, Lindner Heike, Jones Daniel S, Grossniklaus Ueli (2015), Functional analysis of related CrRLK1L receptor-like kinases in pollen tube reception., in EMBO reports
, 16(1), 107-15.
Schmidt Anja, Schmid Marc W, Grossniklaus Ueli (2015), Plant germline formation: common concepts and developmental flexibility in sexual and asexual reproduction., in Development (Cambridge, England)
, 142(2), 229-41.
Schmid Marc W, Grossniklaus Ueli (2015), Rcount: simple and flexible RNA-Seq read counting., in Bioinformatics (Oxford, England)
, 31(3), 436-7.
Lindner Heike, Kessler Sharon A, Müller Lena M, Shimosato-Asano Hiroko, Boisson-Dernier Aurélien, Grossniklaus Ueli (2015), TURAN and EVAN mediate pollen tube reception in Arabidopsis Synergids through protein glycosylation., in PLoS biology
, 13(4), 1002139-1002139.
Ngo Quy A, Vogler Hannes, Lituiev Dmytro S, Nestorova Anna, Grossniklaus Ueli (2014), A calcium dialog mediated by the FERONIA signal transduction pathway controls plant sperm delivery., in Developmental cell
, 29(4), 491-500.
Schmidt Anja, Schmid Marc W, Klostermeier Ulrich C, Qi Weihong, Guthörl Daniela, Sailer Christian, Waller Manuel, Rosenstiel Philip, Grossniklaus Ueli (2014), Apomictic and sexual germline development differ with respect to cell cycle, transcriptional, hormonal and epigenetic regulation., in PLoS genetics
, 10(7), 1004476-1004476.
Grossniklaus Ueli, Paro Renato (2014), Transcriptional silencing by polycomb-group proteins., in Cold Spring Harbor perspectives in biology
, 6(11), 019331-019331.
Boisson-Dernier Aurélien, Lituiev Dmytro S, Nestorova Anna, Franck Christina Maria, Thirugnanarajah Sharme, Grossniklaus Ueli (2013), ANXUR receptor-like kinases coordinate cell wall integrity with growth at the pollen tube tip via NADPH oxidases., in PLoS biology
, 11(11), 1001719-1001719.
Raissig Michael T, Gagliardini Valeria, Jaenisch Johan, Grossniklaus Ueli, Baroux Célia (2013), Efficient and rapid isolation of early-stage embryos from Arabidopsis thaliana seeds., in Journal of visualized experiments : JoVE
, (76), ---.
Raissig Michael T, Bemer Marian, Baroux Célia, Grossniklaus Ueli (2013), Genomic imprinting in the Arabidopsis embryo is partly regulated by PRC2., in PLoS genetics
, 9(12), 1003862-1003862.
Schmidt Anja, Wöhrmann Heike J P, Raissig Michael T, Arand Julia, Gheyselinck Jacqueline, Gagliardini Valeria, Heichinger Christian, Walter Joern, Grossniklaus Ueli (2013), The Polycomb group protein MEDEA and the DNA methyltransferase MET1 interact to repress autonomous endosperm development in Arabidopsis., in The Plant journal : for cell and molecular biology
, 73(5), 776-87.
Grossniklaus Ueli, Kelly William G, Kelly Bill, Ferguson-Smith Anne C, Pembrey Marcus, Lindquist Susan (2013), Transgenerational epigenetic inheritance: how important is it?, in Nature reviews. Genetics
, 14(3), 228-35.
You Wanhui, Tyczewska Agata, Spencer Matthew, Daxinger Lucia, Schmid Marc W, Grossniklaus Ueli, Simon Stacey A, Meyers Blake C, Matzke Antonius J M, Matzke Marjori (2012), Atypical DNA methylation of genes encoding cysteine-rich peptides in Arabidopsis thaliana., in BMC plant biology
, 12, 51-51.
Lindner Heike, Müller Lena Maria, Boisson-Dernier Aurélien, Grossniklaus Ueli (2012), CrRLK1L receptor-like kinases: not just another brick in the wall., in Current opinion in plant biology
, 15(6), 659-69.
Bemer Marian, Grossniklaus Ueli (2012), Dynamic regulation of Polycomb group activity during plant development., in Current opinion in plant biology
, 15(5), 523-9.
Sprunck Stefanie, Rademacher Svenja, Vogler Frank, Gheyselinck Jacqueline, Grossniklaus Ueli, Dresselhaus Thomas (2012), Egg cell-secreted EC1 triggers sperm cell activation during double fertilization., in Science (New York, N.Y.)
, 338(6110), 1093-7.
Wöhrmann Heike J P, Gagliardini Valeria, Raissig Michael T, Wehrle Wendelin, Arand Julia, Schmidt Anja, Tierling Sascha, Page Damian R, Schöb Hanspeter, Walter Jörn, Grossniklaus Ueli (2012), Identification of a DNA methylation-independent imprinting control region at the Arabidopsis MEDEA locus., in Genes & development
, 26(16), 1837-50.
Lindner Heike, Raissig Michael T, Sailer Christian, Shimosato-Asano Hiroko, Bruggmann Rémy, Grossniklaus Ueli (2012), SNP-Ratio Mapping (SRM): identifying lethal alleles and mutations in complex genetic backgrounds by next-generation sequencing., in Genetics
, 191(4), 1381-6.
Marshall Alex, Aalen Reidunn B, Audenaert Dominique, Beeckman Tom, Broadley Martin R, Butenko Melinka A, Caño-Delgado Ana I, de Vries Sacco, Dresselhaus Thomas, Felix Georg, Graham Neil S, Foulkes John, Granier Christine, Greb Thomas, Grossniklaus Ueli, Hammond John P, Heidstra Renze, Hodgman Charlie, Hothorn Michael, Inzé Dirk, Ostergaard Lars, Russinova Eugenia, Simon Rüdiger, Skirycz Aleksandra, Stahl Yvonne (2012), Tackling drought stress: receptor-like kinases present new approaches., in The Plant cell
, 24(6), 2262-78.
Ngo Quy A, Baroux Celia, Guthörl Daniela, Mozerov Peter, Collinge Margaret A, Sundaresan Venkatesan, Grossniklaus Ueli (2012), The Armadillo repeat gene ZAK IXIK promotes Arabidopsis early embryo and endosperm development through a distinctive gametophytic maternal effect., in The Plant cell
, 24(10), 4026-43.
Gametogenesis and the maternal control of embryogenesis have been studied extensively in animal systems at the genetic, molecular, and cellular level. These studies have yielded insight into fundamental concepts and mechanism involved in the establishment and interpretation of positional information, cell-cell interaction, and signal transduction cascades. In contrast, our knowledge about genetic and molecular control of these processes in plants is very limited. The simple but highly polar and specialized female gametophyte, which consists of only seven cells belonging to four cell types, make it an ideal system to study the role of positional information and signaling during development. Using Arabidopsis thaliana as a model system, this proposal focuses on the elucidation of key events during sexual reproduction, namely cell specification, fertilization, and the epigenetic control of seed development with a focus on embryogenesis. We will use a combination of (quantitative) genetic, cell biological, molecular, and computational approaches. Many of the methods are established in the laboratory and rely on standard genetic and molecular biology protocols. Others need special adaptations and have been optimized for the study of the elusive and inaccessible female gametophyte. However, also novel experimental approaches will be established and adapted to our system, such as (i) methods to investigate sister chromatid segregation, (ii) network analyses of cell type-specific transcriptomes; (iii) novel cloning approaches using next generation sequencing technology, (iv) genome-wide association studies to identify modifier loci, and (v) the development of genetic and transgenic tools to investigate embryogenesis uninfluenced by the endosperm. The experiments described in this proposal will shed light on fundamental aspects of plant development, such as the role of positional information, cell-cell interactions, signal transduction, and epigenetic gene regulation in development. Thus, they span numerous fields of developmental biology and will yield novel insights into developmental control mechanisms that transcend plant biology. For example, epigenetic mechanisms play an important role in growth and development. While the role of epigenetic gene regulation in cancer and disease has only become apparent over the last years, plant researchers have studied epigenetic phenomena for decades and have contributed much to our understanding of the underlying molecular mechanisms.