Project

Back to overview

Metabolism of polar organic xenobiotics in aquatic organisms: importance and contribution to environmental fate

English title Metabolism of polar organic xenobiotics in aquatic organisms: importance and contribution to environmental fate
Applicant Hollender Juliane
Number 141190
Funding scheme Project funding
Research institution Swiss Federal Institute of Aquatic Science and Technology (EAWAG)
Institution of higher education Swiss Federal Institute of Aquatic Science and Technology - EAWAG
Main discipline Environmental Research
Start/End 01.02.2013 - 31.03.2016
Approved amount 375'000.00
Show all

Keywords (8)

metabolism; transformation products; biotransformation; algae; daphnia; gammarus; organic contaminants; HPLC-HRMS

Lay Summary (German)

Lead
Wissen über die Biotransformation von organischen Verbindungen in Organismen der aquatischen Nahrungskette ist essentiell, um die Exposition der Organismen in der Umwelt mit dem toxischen Effekt mechanistisch zu verknüpfen. Nur mit Hilfe dieses Wissens können Umweltqualitätsstandards im Rahmen der Wasserrahmenrichtlinie wissenschaftlich abgeleitet werden sowie das Bioakkumulationspotential evaluiert werden.
Lay summary

Das übergeordnete Zieldes Projektes ist es, die Biotransformation von polaren organischenVerbindungen in aquatischen Organismen zu charakterisieren, ihren Einfluss aufdie Bioakkumulation zu bestimmen sowie ihren Beitrag für den Verbleib derVerbindungen in der aquatischen Umwelt. Im Detail werdenBiotransformationsprodukte für ein Set von organischen Verbindungen wieausgewählte Pharmazeutika und Pestizide unter Nutzen der Flüssigchromatographiemit hochauflösenden Massenspektrometrie identifiziert, quantifiziert und die Bildungskinetikmodelliert. Dies ermöglicht es, den Zusammenhang zwischen chemischenEigenschaften und Biotransformation besser zu verstehen. Um Speziesunterschiedeabzudecken, werden Algen, Daphnien und Gammariden als wichtige Vertreterverschiedener Stufen der Nahrungskette untersucht. Für die Algen soll darüberhinaus bestimmt werden, inwiefern sie neben abiotischen und mikrobiellenProzessen zur Entfernung der organischen Stoffe in der aquatischen Umweltbeitragen und daher stärker in die Risikobewertung mit einbezogen werdensollten.  

Direct link to Lay Summary Last update: 22.01.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
How Biotransformation Influences Toxicokinetics of Azole Fungicides in the Aquatic Invertebrate Gammarus pulex
Rösch Andrea, Anliker Sabine, Hollender Juliane (2016), How Biotransformation Influences Toxicokinetics of Azole Fungicides in the Aquatic Invertebrate Gammarus pulex, in Environmental Science & Technology, 50(13), 7175-7188.
Microvolume trace environmental analysis using peak-focusing online solid-phase extraction–nano-liquid chromatography–high-resolution mass spectrometry
Stravs Michael, Mechelke Jonas, Ferguson Lee, Singer Heinz, Hollender Juliane (2016), Microvolume trace environmental analysis using peak-focusing online solid-phase extraction–nano-liquid chromatography–high-resolution mass spectrometry, in Analytical and bioanalytical chemistry, 408, 1879-1890.
Biotransformation pathways of biocides and pharmaceuticals in freshwater crustaceans based on structure elucidation of metabolites using high resolution mass spectrometry
Jeon Junho, Kurth Denise, Hollender Juliane (2013), Biotransformation pathways of biocides and pharmaceuticals in freshwater crustaceans based on structure elucidation of metabolites using high resolution mass spectrometry, in Chemical Research in Toxicology, (3), 313-324.
Comparative toxicokinetics of organic micropollutants in freshwater crustaceans
Jeon Junho, Kurth Denise, Ashauer Roman, Hollender Juliane (2013), Comparative toxicokinetics of organic micropollutants in freshwater crustaceans, in Environmental Science and Technology, (15), 8809-8817.

Collaboration

Group / person Country
Types of collaboration
Prof. Dr. Beate Escher, EnTox, Brisbane Australia (Oceania)
- in-depth/constructive exchanges on approaches, methods or results
PD Dr. Piet Spaak, Aquatic Ecology Eawag Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Publication
Dr. Renata Behra, Environmental Toxicology, Eawag Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Dr. Roman Ashauer, Enironmental Toxicology, Eawag Switzerland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Exchange of personnel

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
15th EuCheMS International Conference on Chemistry and the Environment Poster Biotransformation of azole fungicides in the aquatic invertebrate Gammarus pulex 20.09.2015 Leipzig, Germany Hollender Juliane; Rösch Andrea Barbara;
20. Jahrestagung der SETAC German language branch Poster Biotransformation ausgewählter Azolfungizide im Flohkrebs Gammarus pulex und deren Wirkung in Fungizidmischungen 07.09.2015 Zürich, Switzerland Hollender Juliane; Rösch Andrea Barbara;
20. Jahrestagung der Setac GLB Poster Bioakkumulation von polaren Mikroverunreinigungen in Phytokplankton-Spezies 07.09.2015 Zürich, Switzerland Pomati Francesco; Hollender Juliane; Stravs Michael;
25th Annual conference of the European Society of Environmental Toxicology and Chemistry (SETAC) Talk given at a conference Biotransformation of selected azole fungicides in the aquatic invertebrate Gammarus pulex and their effects on oxidative drug metabolism 03.05.2015 Barcelona, Spain Rösch Andrea Barbara; Hollender Juliane;
25th Annual European SETAC conference Poster Bioaccumulation of polar organic micropollutants in phytoplankton species 03.05.2015 Barcelona, Spain Pomati Francesco; Hollender Juliane; Stravs Michael;
32nd Swiss Group for Mass Spectrometry meeting Poster RMassBank: a workflow for automated processing, recalibration and annotation of high resolution mass spectra for the MassBank spectral library 30.10.2014 Beatenberg, Switzerland Stravs Michael; Hollender Juliane;
20th International Mass Spectrometry Conference Talk given at a conference Online SPE-nano-LC-MS for the analysis of trace organic contaminants in environmental samples 24.08.2014 Geneva, Switzerland Stravs Michael; Hollender Juliane;
24th Annual European SETAC conference Poster Biotransformation of fungicides in the aquatic invertebrate Gammarus pulex 11.05.2014 Basel, Switzerland Hollender Juliane; Rösch Andrea Barbara;
24th Annual European SETAC conference Poster Online SPE-nano-LC-HRMS of polar organic contaminants in environmental samples 11.05.2014 Basel, Switzerland Stravs Michael; Hollender Juliane;


Awards

Title Year
Best poster awards at the conference of the Swiss Group of Mass Spectrometry 2014

Associated projects

Number Title Start Funding scheme
150638 Empa/Eawag NMR Spektrometer, Aufrüstung mit einem Probenkopf des Typs Prodigy Cryo 01.04.2014 R'EQUIP
125034 Development of Passive Sampling for Water Quality Assessment and for Simulating the Bioconcentration in Aquatic Organism Under Pulsed Exposure Conditions 01.01.2009 Project funding
165935 Bioaccumulation and biotransformation of organic xenobiotics in aquatic organisms 01.04.2016 Project funding

Abstract

Knowledge on the importance of biotransformation of polar organic compounds in invertebrates of the aquatic food web is essential to mechanistically link environmental exposure with toxic effects but currently rather limited. The metabolic profiles, the enzymatic activities involved and the identity of transformation products are not known in most cases although this is necessary to determine whether the metabolism re-sults in bioactivation or detoxification of xenobiotics. This is important for scientific derivation of environ-mental quality standards in the context of the water framework directive as well as the evaluation of the bio-accumulation potential for registration of new chemicals within REACH. The overall goal of the project is to characterize the biotransformation of polar compounds in aquatic inverte-brates and determine the importance for bioaccumulation as well as the contribution to fate in the aquatic environment. Specifically, we want to answer the following research questions: 1. What does biotransformation look like in aquatic invertebrates with regard to enzymatic processes in-volved and biotransformation products (BTPs) formed?2. How is the biotransformation activity linked with chemical properties? 3. To what extent does the bioaccumulation and biotransformation in algal communities contributes to the fate in the aquatic environment? We plan to use a set of polar organic compounds such as pharmaceuticals, biocides and pesticides with a variety of physicochemical properties, functional groups, and half-lives in the environment to explore the va-riety of enzymatic processes in organisms and the influence of the molecule structure on biotransformation. In order to account for species differences, we selected three model organisms from different trophic levels which are focal organisms for the functioning of aquatic food webs and already used in the labs of the appli-cants (Daphnia magna, Gammarus pulex and freshwater algal species). Two types of exposure experiments with single species are anticipated, one for BTP screening and another time-course experiment for the determination of toxicokinetic parameters. One important part of the proposal is the identification of stable BTPs following extraction, enrichment, purification, and liquid chromatography combined with high resolution mass spectrometry. Therefore suspect screening of BTPs predicted by path-way prediction software, as well as non-target screening by comparison of treatments and controls using heuristic rules and databases will be applied. In vitro enzymatic assays with biota extracts will be used to support the elucidation of BTPs and characterize the phase-I and -II reactions. Following this, parent com-pounds and BTPs are quantified in exposure and depuration experiments and the toxicokinetic parameters for uptake, biotransformation, and elimination will be modeled assuming first-order kinetics. The identity of the BTPs and comparison of the toxicokinetic rates for the different compounds in the different organisms, as well as with literature data, will be used to answer research questions 1 and 2. In order to address research question 3, we aim to study the importance of bioaccumulation and biotrans-formation in algal species for the fate in the aquatic environment. Until now, besides abiotic processes such as photolysis and sorption, only biodegradation by heterotrophic bacteria is taken into account for environ-mental fate. While phytoremediation is well known, biotransformation by algal species remains almost un-studied although algae occur ubiquitously and in high quantities in the aquatic environment. We will perform laboratory degradation tests using single species and mixtures, following this up with field collection and ex-periments using natural algal communities. Parent compounds and stable BTPs will be quantified in media and biota over time. Comparison of the dissipation and degradation half-lives indicates the influence of the community biodiversity on the degradation pattern. Comparison with known transformation half-lives for abiotic and microbial processes will reveal the importance of the algal communities on the fate in the natural environment.
-