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Priming plants for biotic and abiotic stress resistance:investigating possible mechanisms

English title Priming plants for biotic and abiotic stress resistance:investigating possible mechanisms
Applicant Mauch-Mani Brigitte
Number 140593
Funding scheme Project funding
Research institution Lab. de Biologie Moléculaire et Cellulaire Institut de Biologie Université de Neuchâtel
Institution of higher education University of Neuchatel - NE
Main discipline Botany
Start/End 01.05.2012 - 30.04.2015
Approved amount 502'000.00
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All Disciplines (3)

Discipline
Botany
Genetics
Molecular Biology

Keywords (3)

transgeneration priming; duration of priming; induced resistance

Lay Summary (German)

Lead
Lay summary

Unsere Forschung hat aufgezeigt, dass Pflanzen welche mit einer Resistenz-induzierenden Chemikalie (beta Aminobuttersäure) oder auch mit avirulenten Bakterien behandelt werden resistenter gegen die Angriffe von Pathogenen Organismen werden und auch abiotischen Stress wie zum Beispiel Trockenheit besser aushalten können. Interessanterweise wird diese erhöhte Resistenz von den Elternpflanzen an die Nachkommen weitergegeben. Wir untersuchen, welche Mechanismen dieser Art von Vererbung zugrunde liegen könnten.

Das Behandeln von Elternpflanzen mit dem Ziel sich besser wehrende Nachkommen zu erzeugen hätte grosse Vorteile für die Landwirtschaft da dies zu einer Verringerung des Pestizideinsatzes führen könnte

Direct link to Lay Summary Last update: 21.02.2013

Responsible applicant and co-applicants

Employees

Publications

Publication
Beta-aminobutyric acid priming of plant defense: the role of ABA and other hormones
Baccelli Ivan, Mauch-Mani Brigitte (2016), Beta-aminobutyric acid priming of plant defense: the role of ABA and other hormones, in Plant Molecular Biology, 703.
Preparing to fight back: generation and storage of priming compounds.
Pastor Victoria, Balmer Andrea, Gamir Jordi, Flors Victor, Mauch-Mani Brigitte (2014), Preparing to fight back: generation and storage of priming compounds., in Frontiers in plant science, 5, 295-295.
Soil drench treatment with β-aminobutyric acid increases drought tolerance of potato
Sós-Hegedus Anita, Juhász Zsófia, Poór Péter, Kondrák Mihály, Antal Ferenc, Tari Irma, Mauch-Mani Brigitte, Bánfalvi Zsófia (2014), Soil drench treatment with β-aminobutyric acid increases drought tolerance of potato, in PLoS ONE, 9(12), e114297.
β-Aminobutyric acid (BABA)-induced resistance in Arabidopsis thaliana: link with iron homeostasis.
Koen Emmanuel, Trapet Pauline, Brulé Daphnée, Kulik Anna, Klinguer Agnès, Atauri-Miranda Livia, Meunier-Prest Rita, Boni Gilles, Glauser Gaétan, Mauch-Mani Brigitte, Wendehenne David, Besson-Bard Angélique (2014), β-Aminobutyric acid (BABA)-induced resistance in Arabidopsis thaliana: link with iron homeostasis., in Molecular plant-microbe interactions : MPMI, 27(11), 1226-40.
Role of two UDP-Glycosyltransferases from the L group of arabidopsis in resistance against pseudomonas syringae
Boachon Benoît, Gamir Jordi, Pastor Victoria, Erb Matthias, Erb Matthias, Dean John V., Flors Víctor, Mauch-Mani Brigitte (2013), Role of two UDP-Glycosyltransferases from the L group of arabidopsis in resistance against pseudomonas syringae, in European Journal of Plant Pathology, 139(4), 707-720.
Descendants of primed Arabidopsis plants exhibit resistance to biotic stress.
Slaughter Ana, Daniel Xavier, Flors Victor, Luna Estrella, Hohn Barbara, Mauch-Mani Brigitte (2012), Descendants of primed Arabidopsis plants exhibit resistance to biotic stress., in Plant physiology, 158(2), 835-43.
Detection, characterization and quantification of salicylic acid conjugates in plant extracts by ESI tandem mass spectrometric techniques.
Pastor Victoria, Vicent Cristian, Cerezo Miguel, Mauch-Mani Brigitte, Dean John, Flors Victor (2012), Detection, characterization and quantification of salicylic acid conjugates in plant extracts by ESI tandem mass spectrometric techniques., in Plant physiology and biochemistry : PPB / Société française de physiologie végétale, 53, 19-26.
Small yet mighty - microRNAs in plant-microbe interactions
Balmer Dirk, Mauch-Mani Brigitte (2012), Small yet mighty - microRNAs in plant-microbe interactions, in MicroRNA (Shāriqah, United Arab Emirates), 2(1), 72-79.
The 'prime-ome': Towards a holistic approach to priming
Balmer Andrea, Pastor Victoria, Gamir Jordi, Flors Victor, Mauch-Mani Brigitte, The 'prime-ome': Towards a holistic approach to priming, in Trends in Plant Science.

Collaboration

Group / person Country
Types of collaboration
Flors group/University Jaume I, Castellon Spain (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
Agroscope Switzerland (Europe)
- Research Infrastructure
University of Sheffield, J Ton's group Great Britain and Northern Ireland (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Momcilovic group/Institute for Biological Research, University of Belgrade Serbien (Europe)
- in-depth/constructive exchanges on approaches, methods or results
Edmund Mach Foundation, San Michele all'Adige Italy (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Exchange of personnel
Department of Biological Sciences, dePaul University, Chicago, J. Dean United States of America (North America)
- in-depth/constructive exchanges on approaches, methods or results
Banfalvi group/Agricultural Biotechnology Center, Gödöllo Hungary (Europe)
- in-depth/constructive exchanges on approaches, methods or results
- Research Infrastructure
- Exchange of personnel

Scientific events

Active participation

Title Type of contribution Title of article or contribution Date Place Persons involved
XVI International Congress on Molecular Plant-Microbe Interactions Poster Metabolomics of BABA-priming 06.07.2014 Rhodes, Greece Pastore Fuentes Victoria;
Invited seminar Individual talk The role of BABA in priming 13.09.2013 Malmö, Sweden Mauch-Mani Brigitte;
IOBC Meeting: Induced resistance in plants against insects and diseases: leaping from success in the lab to success in the field Talk given at a conference Induced resistance and food safety 11.06.2013 Avignon, France Balmer Dirk; Pastore Fuentes Victoria; Mauch-Mani Brigitte;
Annual Congress of the Serbian Plant Physiology Society Talk given at a conference Insights into induced resistance in monocots 04.06.2013 Subotica, Serbien Mauch-Mani Brigitte;
1st International Forum in Inclusive Global Consciousness Talk given at a conference Role of priming in feeding the world 23.05.2013 Castellon, Spain Mauch-Mani Brigitte;
Institute seminar Individual talk BABA priming 09.04.2013 München, Germany Mauch-Mani Brigitte;
VI Brazilian Meeting on Induced Resistance in Plants against Pathogens Talk given at a conference Priming and Induced resistance 19.11.2012 Viçosa, Brazil Mauch-Mani Brigitte; Balmer Dirk;
Institute Seminar Individual talk Priming in Plant pathogen Interactions 28.09.2012 Zürich, Switzerland Mauch-Mani Brigitte;
SEB Meeting Talk given at a conference The progeny of chemically-primed plants is better protected against biotic and abiotic stresses 29.06.2012 Salzburg, Austria Mauch-Mani Brigitte; Balmer Dirk;
Institute seminar Individual talk Induced resistence in maize 04.06.2012 Gödöllö, Hungary Balmer Dirk;


Associated projects

Number Title Start Funding scheme
128031 Priming of heat and drought tolerance in potato 01.01.2010 SCOPES
120197 Characterization of arabidopsis mutants altered in their ability to express BABA-induced resistance (BABA-IR) and priming 01.05.2008 Project funding

Abstract

Plants treated with the non-protein amino acid beta-aminobutyric acid (BABA) develop an enhanced defensive capacity against a large variety of biotic and abiotic stresses. The expression of this BABA-induced resistance (BABA-IR) coincides with a faster and stronger defense response following pathogen attack or abiotic stress. This phenomenon has been termed “priming, sensitization or potentiation”, and the underlying mechanisms are starting to become clearer. Priming has been proposed to be based on the accumulation of signalling proteins in an inactive form that would then be activated upon exposure to stress. Another suggestion was the accumulation of transcription factors in primed plants leading to an enhanced defense gene transcription when this plant is stressed and, recently, evidence has been pointing to changes at the epigenetic level. We have previously isolated mutants of Arabidopsis impaired in the expression of BABA-IR. Besides demonstrating that there is a genetic basis for the observed induced resistance and priming, the analysis of these mutants also allowed to start dissecting the signalling pathways involved in the phenomenon, demonstrating that BABA-IR against biotic and abiotic stresses is based on priming of distinct defense signaling mechanisms involving salicylic acid-, abscisic acid-, and phosphoinositide-dependent pathways and that callose deposition plays an important role. Here, we propose to take advantage of the recently established Chemical Analysis Platform at our university and ensuing expertise to look at the fate of BABA in plants, i.e. determine its perseverance, concentration and possible catabolism and to develop a sensitive, accurate methodology to determine hormones and their conjugates as well as other metabolite levels in plants. Such methods will enable us to determine the role of fine-tuning of the hormonal homeostasis during BABA-IR and induced resistance in general. Conjugation of hormones is a way of controlling the cellular level of these substances and we have preliminary results showing that conjugation reactions might contribute to BABA-IR as well as basic immunity of Arabidopsis against Pseudomonas syringae.In a second approach we propose to investigate the duration of induced resistance and priming after an inducing treatment. Our own results as well as recent presentations by colleagues at scientific meetings point to the possibility that the primed state might be transferred to the descendants of primed plants. Here we propose to establish whether this is the case for different IR systems and against various biotic and abiotic stresses. We plan to screen WT populations of Arabidopsis for accessions showing a “hypo- or hyper-long-time priming” phenotype in view of establishing the genetic basis. We will also investigate the dependency of the phenomenon on defined signalling pathways and genes known to be central for the expression of IR. Finally, we will determine whether there is a possibility that the observed phenomenon is due to transfer of BABA or derivates, or of an eventual bacteria-generated or induced molecule through the seeds to the next generation. If the observed trans-generation priming can be unequivocally attributed to a transfer of the primed state to the descendants of a plant, our results and generated plant populations will be the starting point for later investigations into the molecular mechanisms behind the observed phenomenon.Priming-inducing events or treatments conferring enhanced resistance against both biotic and abiotic stresses with minimal inhibitory effects on commercially important traits are of interest to have a means of transferring useful adaptations to a following generation, both in natural as in agricultural ecosystems.
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